Analysis Of Pyronaridine And Pyronaridine Tetraphosphate Using High Performance Liquid Chromatography And High Resolution Nuclear Magnetic Resonance Spectroscopy

Pironaridina (PND), 2-metoksi-7 -kloro-1 0-[3' ,5'-bis-(pyrrolidinil-1-metil)-4'- hidroksianinilo]benzo[b]-1,5-naftiridina (formula molekul: C29H32CIN502) yang disintesiskan oleh Zheng et al pada awal 1970an telah digunakan sebagai ubat antimalaria selama 20 tahun. la berwarna mera...

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Main Author:	Saurajen Pillay, Shubhadra Pillay
Format:	Thesis
Language:	English
Published:	2006
Subjects:	R735-854 Medical education Medical schools Research
Online Access:	http://eprints.usm.my/31207/1/SHUNHADRA_PILLAY_A.P_SAURAJEN_PILLAY.pdf
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spelling	my-usm-ep.312072016-11-28T02:25:22Z Analysis Of Pyronaridine And Pyronaridine Tetraphosphate Using High Performance Liquid Chromatography And High Resolution Nuclear Magnetic Resonance Spectroscopy 2006-07 Saurajen Pillay, Shubhadra Pillay R735-854 Medical education. Medical schools. Research Pironaridina (PND), 2-metoksi-7 -kloro-1 0-[3' ,5'-bis-(pyrrolidinil-1-metil)-4'- hidroksianinilo]benzo[b]-1,5-naftiridina (formula molekul: C29H32CIN502) yang disintesiskan oleh Zheng et al pada awal 1970an telah digunakan sebagai ubat antimalaria selama 20 tahun. la berwarna merah bata, dengan rasa pahit dan tidak berbau dengan takat lebur pada suhu 174-176 °C. Bagi tujuan formulasi ubat, PND diubah kepada terbitan fosfatnya iaitu pironaridina tetrafosfat (PNDT) yang terlarut dalam air (formula molekul: C29H32CIN502.4H3P04) dan mempunyai takat lebur pada 227-230 °C. Kajian yang dilakukan ke atas PNDT sebelum ini hanya menunjukkan satu puncak pad a kromatogram apabila dianalisis dengan menggunakan kromatografi cecair prestasi tinggi (KGPT). Tetapi, dengan menggunakan kaedah terubahsuai Jayaraman yang dikembangkan oleh Karupiah (2003), tiga puncak diperhatikan pada kromatogram. Tiga puncak ini dilabel sebagai komponen B pad a 10.0 min, X pada 21.2 min dan Y pad a 24.3 min. Sebatian B diekstrak dan pencirian struktur sebatian tersebut dilakukan. Akan tetapi, untuk memastikan identiti komponen B, informasi struktur PND dan PNDT perlu ditentukan. Sehingga sekarang, data NMR yang wujud hanya daripada spektrum yang dirakam pada 2.11 Tesla (T) (90 MHz untuk 1H). Pyronaridine (PND), 2-methoxy-7 -chloro-1 0-[3' ,S'-bis-(pyrrolidinyl-1-methyl)-4'hydroxyanilino] benzo[b]-1 ,S-napthyridines (molecular formula: C29H32CINs02), synthesized by Zheng et al. in the early 1970s, has been used in China for over 20 years as an antimalarial drug. It is an odourless, brick red powder with a bitter taste and decomposes at 174-176 °c. For drug formulation purposes, PND is converted to its water-soluble tetraphosphate derivative, PNDT (molecular formula: C29H32CINs02.4H3P04) that has a melting point of 227-230 °c. All previous studies conducted on PND and PNDT using HPLC showed only one peak present in the chromatogram. However, the modified version of Jayaraman's HPLC method developed by Karupiah (2003) showed the presence of three peaks. These were labelled as component B at 10.0 min, X at 21.2 min and Y at 24.3 min. Component B was then extracted and characterized. However, in order to ascertain its identity, detailed structural information of both PND and PNDT must first be established. To date, the only NMR data available in literature on PND was deduced from a proton spectrum recorded at 2.11 Tesla (T) (90 MHz in lH). 2006-07 Thesis http://eprints.usm.my/31207/ http://eprints.usm.my/31207/1/SHUNHADRA_PILLAY_A.P_SAURAJEN_PILLAY.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Penyelidikan Dadah & Ubat-ubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R735-854 Medical education Medical schools Research
spellingShingle	R735-854 Medical education Medical schools Research Saurajen Pillay, Shubhadra Pillay Analysis Of Pyronaridine And Pyronaridine Tetraphosphate Using High Performance Liquid Chromatography And High Resolution Nuclear Magnetic Resonance Spectroscopy
description	Pironaridina (PND), 2-metoksi-7 -kloro-1 0-[3' ,5'-bis-(pyrrolidinil-1-metil)-4'- hidroksianinilo]benzo[b]-1,5-naftiridina (formula molekul: C29H32CIN502) yang disintesiskan oleh Zheng et al pada awal 1970an telah digunakan sebagai ubat antimalaria selama 20 tahun. la berwarna merah bata, dengan rasa pahit dan tidak berbau dengan takat lebur pada suhu 174-176 °C. Bagi tujuan formulasi ubat, PND diubah kepada terbitan fosfatnya iaitu pironaridina tetrafosfat (PNDT) yang terlarut dalam air (formula molekul: C29H32CIN502.4H3P04) dan mempunyai takat lebur pada 227-230 °C. Kajian yang dilakukan ke atas PNDT sebelum ini hanya menunjukkan satu puncak pad a kromatogram apabila dianalisis dengan menggunakan kromatografi cecair prestasi tinggi (KGPT). Tetapi, dengan menggunakan kaedah terubahsuai Jayaraman yang dikembangkan oleh Karupiah (2003), tiga puncak diperhatikan pada kromatogram. Tiga puncak ini dilabel sebagai komponen B pad a 10.0 min, X pada 21.2 min dan Y pad a 24.3 min. Sebatian B diekstrak dan pencirian struktur sebatian tersebut dilakukan. Akan tetapi, untuk memastikan identiti komponen B, informasi struktur PND dan PNDT perlu ditentukan. Sehingga sekarang, data NMR yang wujud hanya daripada spektrum yang dirakam pada 2.11 Tesla (T) (90 MHz untuk 1H). Pyronaridine (PND), 2-methoxy-7 -chloro-1 0-[3' ,S'-bis-(pyrrolidinyl-1-methyl)-4'hydroxyanilino] benzo[b]-1 ,S-napthyridines (molecular formula: C29H32CINs02), synthesized by Zheng et al. in the early 1970s, has been used in China for over 20 years as an antimalarial drug. It is an odourless, brick red powder with a bitter taste and decomposes at 174-176 °c. For drug formulation purposes, PND is converted to its water-soluble tetraphosphate derivative, PNDT (molecular formula: C29H32CINs02.4H3P04) that has a melting point of 227-230 °c. All previous studies conducted on PND and PNDT using HPLC showed only one peak present in the chromatogram. However, the modified version of Jayaraman's HPLC method developed by Karupiah (2003) showed the presence of three peaks. These were labelled as component B at 10.0 min, X at 21.2 min and Y at 24.3 min. Component B was then extracted and characterized. However, in order to ascertain its identity, detailed structural information of both PND and PNDT must first be established. To date, the only NMR data available in literature on PND was deduced from a proton spectrum recorded at 2.11 Tesla (T) (90 MHz in lH).
format	Thesis
qualification_level	Master's degree
author	Saurajen Pillay, Shubhadra Pillay
author_facet	Saurajen Pillay, Shubhadra Pillay
author_sort	Saurajen Pillay, Shubhadra Pillay
title	Analysis Of Pyronaridine And Pyronaridine Tetraphosphate Using High Performance Liquid Chromatography And High Resolution Nuclear Magnetic Resonance Spectroscopy
title_short	Analysis Of Pyronaridine And Pyronaridine Tetraphosphate Using High Performance Liquid Chromatography And High Resolution Nuclear Magnetic Resonance Spectroscopy
title_full	Analysis Of Pyronaridine And Pyronaridine Tetraphosphate Using High Performance Liquid Chromatography And High Resolution Nuclear Magnetic Resonance Spectroscopy
title_fullStr	Analysis Of Pyronaridine And Pyronaridine Tetraphosphate Using High Performance Liquid Chromatography And High Resolution Nuclear Magnetic Resonance Spectroscopy
title_full_unstemmed	Analysis Of Pyronaridine And Pyronaridine Tetraphosphate Using High Performance Liquid Chromatography And High Resolution Nuclear Magnetic Resonance Spectroscopy
title_sort	analysis of pyronaridine and pyronaridine tetraphosphate using high performance liquid chromatography and high resolution nuclear magnetic resonance spectroscopy
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Penyelidikan Dadah & Ubat-ubatan
publishDate	2006
url	http://eprints.usm.my/31207/1/SHUNHADRA_PILLAY_A.P_SAURAJEN_PILLAY.pdf
_version_	1747820403391201280

Analysis Of Pyronaridine And Pyronaridine Tetraphosphate Using High Performance Liquid Chromatography And High Resolution Nuclear Magnetic Resonance Spectroscopy

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