Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans

Sifat kepatogenan dalam C. albicans bergantung kepada atribut kecergasan dan juga faktor-faktor virulen yang lain. Ini termasuk sifat kental terhadap tindak balas tekanan dan penyesuaian metabolik. C. albicans boleh menduduki kebanyakkan persekitaran dalam ruang badan manusia yang mengandungi kepelb...

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Main Author:	Ting , Seng Yeat
Format:	Thesis
Language:	English
Published:	2016
Subjects:	RK1-715 Dentistry
Online Access:	http://eprints.usm.my/31499/1/TING_SENG_YEAT_24.pdf
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id	my-usm-ep.31499
record_format	uketd_dc
spelling	my-usm-ep.314992019-04-12T05:25:26Z Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans 2016-02 Ting , Seng Yeat RK1-715 Dentistry Sifat kepatogenan dalam C. albicans bergantung kepada atribut kecergasan dan juga faktor-faktor virulen yang lain. Ini termasuk sifat kental terhadap tindak balas tekanan dan penyesuaian metabolik. C. albicans boleh menduduki kebanyakkan persekitaran dalam ruang badan manusia yang mengandungi kepelbagaian sumber karbon yang berbeza. Asimilasi sumber karbon adalah penting untuk pertumbuhan dan juga untuk meneruskan jangkitan. Tesis ini mengkaji kesan galaktosa atau fruktosa terhadap asimilasi sumber karbon sekunder seperti laktat oleh C. albicans. Gen C. albicans Isositrat liase (CaICL1) ditindas oleh penambahan 2% galaktosa atau fruktosa kepada sel-sel yang sedang membiak pada laktat. Gen CaICL1 yang mengekod kitaran glioksilat enzim Icl1 diperlukan untuk pertumbuhan dalam mengunakan sumber karbon sekunder seperti laktat. Enzim CaIcl1 didapati stabil dalam galaktosa tetapi tidak stabil dalam fruktosa. Sebaliknya, kedua-dua galaktosa dan fruktosa mendegradasikan protein S. cerevisiae Icl1 (ScIcl1). The virulence of C. albicans is dependent upon fitness attributes as well as virulence factors. These attributes include robust stress responses and metabolic flexibility. C. albicans can occupy a variety niches in human, many of which contain a range of different carbon sources. The assimilation of these carbon sources is important for growth and essential for establishment of infections by C. albicans. This thesis examines the impact of galactose or fructose upon the assimilation of secondary carbon sources such as lactate by C. albicans Isocitrate lyase gene (CaICL1) is repressed upon addition of 2% galactose or fructose to lactate- grown cells. The CaICL1 gene, which encode the glyoxylate cycles enzymes isocitrate lyase are required for growth on non- fermentable carbon sources such as lactate. However the enzyme CaIcl1 was not destabilized by galactose, but was degraded in response to fructose. In contrast, S. cerevisiae Icl1 (ScIcl1) was rapidly degraded in response to either galactose or fructose. 2016-02 Thesis http://eprints.usm.my/31499/ http://eprints.usm.my/31499/1/TING_SENG_YEAT_24.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RK1-715 Dentistry
spellingShingle	RK1-715 Dentistry Ting , Seng Yeat Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans
description	Sifat kepatogenan dalam C. albicans bergantung kepada atribut kecergasan dan juga faktor-faktor virulen yang lain. Ini termasuk sifat kental terhadap tindak balas tekanan dan penyesuaian metabolik. C. albicans boleh menduduki kebanyakkan persekitaran dalam ruang badan manusia yang mengandungi kepelbagaian sumber karbon yang berbeza. Asimilasi sumber karbon adalah penting untuk pertumbuhan dan juga untuk meneruskan jangkitan. Tesis ini mengkaji kesan galaktosa atau fruktosa terhadap asimilasi sumber karbon sekunder seperti laktat oleh C. albicans. Gen C. albicans Isositrat liase (CaICL1) ditindas oleh penambahan 2% galaktosa atau fruktosa kepada sel-sel yang sedang membiak pada laktat. Gen CaICL1 yang mengekod kitaran glioksilat enzim Icl1 diperlukan untuk pertumbuhan dalam mengunakan sumber karbon sekunder seperti laktat. Enzim CaIcl1 didapati stabil dalam galaktosa tetapi tidak stabil dalam fruktosa. Sebaliknya, kedua-dua galaktosa dan fruktosa mendegradasikan protein S. cerevisiae Icl1 (ScIcl1). The virulence of C. albicans is dependent upon fitness attributes as well as virulence factors. These attributes include robust stress responses and metabolic flexibility. C. albicans can occupy a variety niches in human, many of which contain a range of different carbon sources. The assimilation of these carbon sources is important for growth and essential for establishment of infections by C. albicans. This thesis examines the impact of galactose or fructose upon the assimilation of secondary carbon sources such as lactate by C. albicans Isocitrate lyase gene (CaICL1) is repressed upon addition of 2% galactose or fructose to lactate- grown cells. The CaICL1 gene, which encode the glyoxylate cycles enzymes isocitrate lyase are required for growth on non- fermentable carbon sources such as lactate. However the enzyme CaIcl1 was not destabilized by galactose, but was degraded in response to fructose. In contrast, S. cerevisiae Icl1 (ScIcl1) was rapidly degraded in response to either galactose or fructose.
format	Thesis
qualification_level	Master's degree
author	Ting , Seng Yeat
author_facet	Ting , Seng Yeat
author_sort	Ting , Seng Yeat
title	Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans
title_short	Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans
title_full	Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans
title_fullStr	Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans
title_full_unstemmed	Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans
title_sort	effects of galactose and fructose on the expression of isocitrate lyase (icl1) enzyme in candida albicans
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute)
publishDate	2016
url	http://eprints.usm.my/31499/1/TING_SENG_YEAT_24.pdf
_version_	1747820437892497408

Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans

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