In Vitro Selection Of Dendrobium Sonia-28 Protocorm-Like Bodies Against Fusarium Proliferatum

Dendrobium sonia-28 adalah hibrid orkid yang penting dalam industri bunga di Malaysia kerana berkeupayaan berbunga berulang kali dan berbunga padat yang kini sedang menghadapi masalah pengeluaran yang serius akibat penyakit disebabkan kulat, terutamanya oleh Fusarium proliferatum. Untuk mengatasi ma...

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Main Author:	Dehgahi, Raheleh
Format:	Thesis
Language:	English
Published:	2016
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/31560/1/RAHELEH_DEHGAHI_24.pdf
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spelling	my-usm-ep.315602019-04-12T05:25:21Z In Vitro Selection Of Dendrobium Sonia-28 Protocorm-Like Bodies Against Fusarium Proliferatum 2016-04 Dehgahi, Raheleh QH1 Natural history (General - Including nature conservation, geographical distribution) Dendrobium sonia-28 adalah hibrid orkid yang penting dalam industri bunga di Malaysia kerana berkeupayaan berbunga berulang kali dan berbunga padat yang kini sedang menghadapi masalah pengeluaran yang serius akibat penyakit disebabkan kulat, terutamanya oleh Fusarium proliferatum. Untuk mengatasi masalah ini, salah satu strategi yang sedang diusahakan adalah dengan penghasilan orkid mutan baru. Mutagenesis secara in vitro dengan cara kultur turasan Fusarium proliferatum (CF), asid fusarik (FA) dan sinaran gama boleh digunakan untuk menghasilkan mutan yang lebih baik dari segi ekonomi. Dalam kajian ini, pemilihan jasad seperti protokom (JSP) yang bertoleransi terhadap F. proliferatum telah dijalankan dengan menilai kesan kepekatan CF (5-20%) dan FA (0.05-0.2 mM) dan pelbagai dos sinaran gama (10-200 Gy). Hasil kajian menunjukkan bahawa kadar hidup dan berat JSP berkadar songsang terhadap inokulasi dan sinaran dos. Selain itu, kadar kematian dan kekurangan berat JSP meningkat di kalangan JSP yang lebih kecil selepas rawatan CF dan FA. Keputusan menunjukkan bahawa ujian sensitiviti radio (LD50) bagi JSP lebih kurang pada 43 Gy. Dendrobium sonia-28 is an important orchid hybrid in Malaysian flower industry for its flowering recurrence and dense inflorescences which currently facing serious production problems due to fungal diseases, especially caused by Fusarium proliferatum. To overcome this impediment, one of the strategies being pursued is by the production of new orchid mutants. In vitro mutagenesis by means of Fusarium proliferatum culture filtrate (CF), fusaric acid (FA) and gamma irradiation can be used to produce economically improved mutants. In this study, selection of Fusarium proliferatum-tolerant protocorm-like bodies (PLBs) was carried out by assessing the effects of different concentrations of CF (5-20%) and FA (0.05-0.2 mM) and various doses of gamma irradiation (10-200 Gy). Results showed that PLBs survival rate and weight were inversely related to the inoculation and irradiation doses. Additionally, PLBs death and weight reducing increased among smaller PLBs after CF and FA treatments. Results indicated that the radio sensitivity test (LD50) for the PLBs was approximately at 43 Gy. 2016-04 Thesis http://eprints.usm.my/31560/ http://eprints.usm.my/31560/1/RAHELEH_DEHGAHI_24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Dehgahi, Raheleh In Vitro Selection Of Dendrobium Sonia-28 Protocorm-Like Bodies Against Fusarium Proliferatum
description	Dendrobium sonia-28 adalah hibrid orkid yang penting dalam industri bunga di Malaysia kerana berkeupayaan berbunga berulang kali dan berbunga padat yang kini sedang menghadapi masalah pengeluaran yang serius akibat penyakit disebabkan kulat, terutamanya oleh Fusarium proliferatum. Untuk mengatasi masalah ini, salah satu strategi yang sedang diusahakan adalah dengan penghasilan orkid mutan baru. Mutagenesis secara in vitro dengan cara kultur turasan Fusarium proliferatum (CF), asid fusarik (FA) dan sinaran gama boleh digunakan untuk menghasilkan mutan yang lebih baik dari segi ekonomi. Dalam kajian ini, pemilihan jasad seperti protokom (JSP) yang bertoleransi terhadap F. proliferatum telah dijalankan dengan menilai kesan kepekatan CF (5-20%) dan FA (0.05-0.2 mM) dan pelbagai dos sinaran gama (10-200 Gy). Hasil kajian menunjukkan bahawa kadar hidup dan berat JSP berkadar songsang terhadap inokulasi dan sinaran dos. Selain itu, kadar kematian dan kekurangan berat JSP meningkat di kalangan JSP yang lebih kecil selepas rawatan CF dan FA. Keputusan menunjukkan bahawa ujian sensitiviti radio (LD50) bagi JSP lebih kurang pada 43 Gy. Dendrobium sonia-28 is an important orchid hybrid in Malaysian flower industry for its flowering recurrence and dense inflorescences which currently facing serious production problems due to fungal diseases, especially caused by Fusarium proliferatum. To overcome this impediment, one of the strategies being pursued is by the production of new orchid mutants. In vitro mutagenesis by means of Fusarium proliferatum culture filtrate (CF), fusaric acid (FA) and gamma irradiation can be used to produce economically improved mutants. In this study, selection of Fusarium proliferatum-tolerant protocorm-like bodies (PLBs) was carried out by assessing the effects of different concentrations of CF (5-20%) and FA (0.05-0.2 mM) and various doses of gamma irradiation (10-200 Gy). Results showed that PLBs survival rate and weight were inversely related to the inoculation and irradiation doses. Additionally, PLBs death and weight reducing increased among smaller PLBs after CF and FA treatments. Results indicated that the radio sensitivity test (LD50) for the PLBs was approximately at 43 Gy.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Dehgahi, Raheleh
author_facet	Dehgahi, Raheleh
author_sort	Dehgahi, Raheleh
title	In Vitro Selection Of Dendrobium Sonia-28 Protocorm-Like Bodies Against Fusarium Proliferatum
title_short	In Vitro Selection Of Dendrobium Sonia-28 Protocorm-Like Bodies Against Fusarium Proliferatum
title_full	In Vitro Selection Of Dendrobium Sonia-28 Protocorm-Like Bodies Against Fusarium Proliferatum
title_fullStr	In Vitro Selection Of Dendrobium Sonia-28 Protocorm-Like Bodies Against Fusarium Proliferatum
title_full_unstemmed	In Vitro Selection Of Dendrobium Sonia-28 Protocorm-Like Bodies Against Fusarium Proliferatum
title_sort	in vitro selection of dendrobium sonia-28 protocorm-like bodies against fusarium proliferatum
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
publishDate	2016
url	http://eprints.usm.my/31560/1/RAHELEH_DEHGAHI_24.pdf
_version_	1747820451327901696

In Vitro Selection Of Dendrobium Sonia-28 Protocorm-Like Bodies Against Fusarium Proliferatum

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