Standardization, Safety And Antiobesity Studies Of Piper Sarmentosum Roxburgh Extracts

Kajian ini merangkumi kawalan kualiti, pemiawaian, keselamatan dan aktiviti biologi menggunakan ekstrak Piper sarmentosum. Ekstrak menggunakan etanol, etanol (50%) dan air telah dipiawai dan dikaji menggunakan assai anti-obesiti dan antihiperlipidemik. Ujian fiziko-kimia, had, cap jari kualitatif...

Full description

Saved in:

Bibliographic Details
Main Author:	Hamil, Mohd Shahrul Ridzuan
Format:	Thesis
Language:	English
Published:	2016
Subjects:	RS1-441 Pharmacy and materia medica
Online Access:	http://eprints.usm.my/31836/1/MOHD_SHAHRUL_RIDZUAN_BIN_HAMIL_24%28NN%29.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.31836
record_format	uketd_dc
spelling	my-usm-ep.318362019-04-12T05:25:22Z Standardization, Safety And Antiobesity Studies Of Piper Sarmentosum Roxburgh Extracts 2016-03 Hamil, Mohd Shahrul Ridzuan RS1-441 Pharmacy and materia medica Kajian ini merangkumi kawalan kualiti, pemiawaian, keselamatan dan aktiviti biologi menggunakan ekstrak Piper sarmentosum. Ekstrak menggunakan etanol, etanol (50%) dan air telah dipiawai dan dikaji menggunakan assai anti-obesiti dan antihiperlipidemik. Ujian fiziko-kimia, had, cap jari kualitatif dan kuantitatif dijalankan menggunakan kaedah spektroskopi dan kromatografi. Kandungan metabolit primer dan sekunder dalam ekstrak ditentukan. Ekstrak telah dianalisis bagi kandungan asaron menggunakan HPLC dan FT-IR. Analisis kemometri (PCA dan HCA) diaplikasikan ke dalam pemiawaianan. Ekstrak terpiawai disaring secara in vitro menggunakan assai sitotoksisiti udang brin (BSLA), perencatan lipase pankreas (PPL) dan aktiviti antiangiogenesis. Assai anti-obesiti dan anti-hiperlipidemik dijalankan secara in vivo dalam tikus teraruh diet tinggi lemak (HFD). This study involves quality control, standardization, safety and biological activities using Piper sarmentosum extracts. Extracts using ethanol, ethanol (50%) and water were standardized and further evaluated using anti-obesity and antihyperlipidemic assays. Physico-chemical, limit test, qualitative and quantitative plant fingerprint was performed using spectroscopic and chromatographic techniques. The contents of primary and secondary metabolites in the extracts were determined. The extracts were analyzed for asarones content using HPLC and FT-IR. Chemometrics (PCA and HCA) were applied in standardization. Standardized extracts were screened in vitro for cytotoxicity against brine shrimp assay (BSLA), pancreatic lipase (PPL) inhibition and anti-angiogenesis activities. The anti-obesity and anti-hyperlipidemic assays were performed in vivo on high fat diet (HFD) induced rats. 2016-03 Thesis http://eprints.usm.my/31836/ http://eprints.usm.my/31836/1/MOHD_SHAHRUL_RIDZUAN_BIN_HAMIL_24%28NN%29.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Farmasi (School of Pharmaceutical Sciences)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RS1-441 Pharmacy and materia medica
spellingShingle	RS1-441 Pharmacy and materia medica Hamil, Mohd Shahrul Ridzuan Standardization, Safety And Antiobesity Studies Of Piper Sarmentosum Roxburgh Extracts
description	Kajian ini merangkumi kawalan kualiti, pemiawaian, keselamatan dan aktiviti biologi menggunakan ekstrak Piper sarmentosum. Ekstrak menggunakan etanol, etanol (50%) dan air telah dipiawai dan dikaji menggunakan assai anti-obesiti dan antihiperlipidemik. Ujian fiziko-kimia, had, cap jari kualitatif dan kuantitatif dijalankan menggunakan kaedah spektroskopi dan kromatografi. Kandungan metabolit primer dan sekunder dalam ekstrak ditentukan. Ekstrak telah dianalisis bagi kandungan asaron menggunakan HPLC dan FT-IR. Analisis kemometri (PCA dan HCA) diaplikasikan ke dalam pemiawaianan. Ekstrak terpiawai disaring secara in vitro menggunakan assai sitotoksisiti udang brin (BSLA), perencatan lipase pankreas (PPL) dan aktiviti antiangiogenesis. Assai anti-obesiti dan anti-hiperlipidemik dijalankan secara in vivo dalam tikus teraruh diet tinggi lemak (HFD). This study involves quality control, standardization, safety and biological activities using Piper sarmentosum extracts. Extracts using ethanol, ethanol (50%) and water were standardized and further evaluated using anti-obesity and antihyperlipidemic assays. Physico-chemical, limit test, qualitative and quantitative plant fingerprint was performed using spectroscopic and chromatographic techniques. The contents of primary and secondary metabolites in the extracts were determined. The extracts were analyzed for asarones content using HPLC and FT-IR. Chemometrics (PCA and HCA) were applied in standardization. Standardized extracts were screened in vitro for cytotoxicity against brine shrimp assay (BSLA), pancreatic lipase (PPL) inhibition and anti-angiogenesis activities. The anti-obesity and anti-hyperlipidemic assays were performed in vivo on high fat diet (HFD) induced rats.
format	Thesis
qualification_level	Master's degree
author	Hamil, Mohd Shahrul Ridzuan
author_facet	Hamil, Mohd Shahrul Ridzuan
author_sort	Hamil, Mohd Shahrul Ridzuan
title	Standardization, Safety And Antiobesity Studies Of Piper Sarmentosum Roxburgh Extracts
title_short	Standardization, Safety And Antiobesity Studies Of Piper Sarmentosum Roxburgh Extracts
title_full	Standardization, Safety And Antiobesity Studies Of Piper Sarmentosum Roxburgh Extracts
title_fullStr	Standardization, Safety And Antiobesity Studies Of Piper Sarmentosum Roxburgh Extracts
title_full_unstemmed	Standardization, Safety And Antiobesity Studies Of Piper Sarmentosum Roxburgh Extracts
title_sort	standardization, safety and antiobesity studies of piper sarmentosum roxburgh extracts
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Farmasi (School of Pharmaceutical Sciences)
publishDate	2016
url	http://eprints.usm.my/31836/1/MOHD_SHAHRUL_RIDZUAN_BIN_HAMIL_24%28NN%29.pdf
_version_	1747820496068542464

Standardization, Safety And Antiobesity Studies Of Piper Sarmentosum Roxburgh Extracts

Similar Items