Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay

Lipase ialah enzim yang digunakan dalam pelbagai aplikasi dan aktivitinya perlu diuji sebelum aplikasi. Namun begitu, kebanyakkan asai aktiviti lipase memerlukan bahan kimia toksik dan persediaan yang rumit. Oleh itu, poli(3-hidroksibutirat-ko-4-hidroksibutirat) [P(3HB-ko-4HB)] digunakan sebagai sub...

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Main Author:	Mok, Pei Shze
Format:	Thesis
Language:	English
Published:	2015
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/31939/1/Mok_Pei_Shze.pdf
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id	my-usm-ep.31939
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spelling	my-usm-ep.319392019-04-12T05:25:38Z Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay 2015-09 Mok, Pei Shze QH1-278.5 Natural history (General) Lipase ialah enzim yang digunakan dalam pelbagai aplikasi dan aktivitinya perlu diuji sebelum aplikasi. Namun begitu, kebanyakkan asai aktiviti lipase memerlukan bahan kimia toksik dan persediaan yang rumit. Oleh itu, poli(3-hidroksibutirat-ko-4-hidroksibutirat) [P(3HB-ko-4HB)] digunakan sebagai substrat pepejal kerana ia dapat diuraikan oleh lipase. Substrat ini boleh diuraikan secara semula jadi dan tidak memerlukan bahan kimia yang bertoksik. Dalam kajian ini, 2.0 ± 0 g/L sel mengandungi 28 ± 2 % of PHA dengan 92 ± 1 mol % of 4HB dalam PHA telah dibiosintesis. Kestabilan filem P(3HB-ko-4HB) dinilai dengan menyimpan filem tersebut di bawah keadaan yang berbeza iaitu di ketuhar vakum, balang pengering dan meja makmal selama 15 bulan. Filem tersebut paling stabil dalam balang pengering yang mengandungi gel silika. Aktiviti depolimerasi lipase komersial dan ekstrak organ haiwan telah diuji secara mikroasai dengan menggunakan P(3HB-ko-4HB) sebagai substrat. Lipase is an enzyme that is widely used in different applications and it is important to screen its activity before application. However, most of the available lipase activity assays require toxic chemicals and tedious preparation. Therefore, a solid substrate known as poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is used and it can be degraded by lipases. This substrate is biodegradable and does not require toxic chemical. In this study, 2.0 ± 0 g/L of cells consisting 28 ± 2 % of PHA with 92 ± 1 mol % of 4HB were produced through biosynthesis. The stability of P(3HB-co-4HB) films was evaluated by storing them in different locations, in vacuum oven, desiccators and bench for 15 months. The film was found to be the most stable in desiccators containing silica gel. The depolymerizing activity of different known commercial lipases and animal organ extracts using P(3HB-co-92 mol% 4HB) as substrate was investigated via microassay. 2015-09 Thesis http://eprints.usm.my/31939/ http://eprints.usm.my/31939/1/Mok_Pei_Shze.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Mok, Pei Shze Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay
description	Lipase ialah enzim yang digunakan dalam pelbagai aplikasi dan aktivitinya perlu diuji sebelum aplikasi. Namun begitu, kebanyakkan asai aktiviti lipase memerlukan bahan kimia toksik dan persediaan yang rumit. Oleh itu, poli(3-hidroksibutirat-ko-4-hidroksibutirat) [P(3HB-ko-4HB)] digunakan sebagai substrat pepejal kerana ia dapat diuraikan oleh lipase. Substrat ini boleh diuraikan secara semula jadi dan tidak memerlukan bahan kimia yang bertoksik. Dalam kajian ini, 2.0 ± 0 g/L sel mengandungi 28 ± 2 % of PHA dengan 92 ± 1 mol % of 4HB dalam PHA telah dibiosintesis. Kestabilan filem P(3HB-ko-4HB) dinilai dengan menyimpan filem tersebut di bawah keadaan yang berbeza iaitu di ketuhar vakum, balang pengering dan meja makmal selama 15 bulan. Filem tersebut paling stabil dalam balang pengering yang mengandungi gel silika. Aktiviti depolimerasi lipase komersial dan ekstrak organ haiwan telah diuji secara mikroasai dengan menggunakan P(3HB-ko-4HB) sebagai substrat. Lipase is an enzyme that is widely used in different applications and it is important to screen its activity before application. However, most of the available lipase activity assays require toxic chemicals and tedious preparation. Therefore, a solid substrate known as poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is used and it can be degraded by lipases. This substrate is biodegradable and does not require toxic chemical. In this study, 2.0 ± 0 g/L of cells consisting 28 ± 2 % of PHA with 92 ± 1 mol % of 4HB were produced through biosynthesis. The stability of P(3HB-co-4HB) films was evaluated by storing them in different locations, in vacuum oven, desiccators and bench for 15 months. The film was found to be the most stable in desiccators containing silica gel. The depolymerizing activity of different known commercial lipases and animal organ extracts using P(3HB-co-92 mol% 4HB) as substrate was investigated via microassay.
format	Thesis
qualification_level	Master's degree
author	Mok, Pei Shze
author_facet	Mok, Pei Shze
author_sort	Mok, Pei Shze
title	Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay
title_short	Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay
title_full	Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay
title_fullStr	Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay
title_full_unstemmed	Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay
title_sort	characterization of the depolymerizing activity of commercial lipases and animal organ extracts using a simple polyhydroxyalkanoate-based microassay
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2015
url	http://eprints.usm.my/31939/1/Mok_Pei_Shze.pdf
_version_	1747820507085930496

Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay

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