Evaluation Of The Anti-Oxidant And Anti-Aging Properties Of Extracts From Four Types Of Plants

Ciri-ciri anti-penuaan ekstrak daripada empat jenis tumbuh-tumbuhan yang berbeza, iaitu O. stamineus, C. hirta, P. sarmentosum dan C. caudatus telah disiasat. Kaedah pengekstrakan yang mudah telah dijalankan sebelum analisis yang seterusnya. Asai DPPH menunjukkan bahawa ekstrak etanol C. hirta me...

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Main Author:	Lim, Ying Yu
Format:	Thesis
Language:	English
Published:	2015
Subjects:	RS1-441 Pharmacy and materia medica
Online Access:	http://eprints.usm.my/31961/1/LIM_YING_YU_24%28NN%29.pdf
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spelling	my-usm-ep.319612019-04-12T05:25:35Z Evaluation Of The Anti-Oxidant And Anti-Aging Properties Of Extracts From Four Types Of Plants 2015-10 Lim, Ying Yu RS1-441 Pharmacy and materia medica Ciri-ciri anti-penuaan ekstrak daripada empat jenis tumbuh-tumbuhan yang berbeza, iaitu O. stamineus, C. hirta, P. sarmentosum dan C. caudatus telah disiasat. Kaedah pengekstrakan yang mudah telah dijalankan sebelum analisis yang seterusnya. Asai DPPH menunjukkan bahawa ekstrak etanol C. hirta menunjukkan aktiviti memerangkap radikal yang tertinggi, dengan IC50 33.3±1.8μg/ml manakala ekstrak etanol P. sarmentosum menunjukkan aktiviti memerangkap radikal yang terendah, dengan IC50 660.4±14.4μg/ml. Toksisiti ekstrak tumbuh-tumbuhan telah diuji dengan menggunakan sel-sel fibroblast kulit manusia dewasa. O. stamineus dan C. caudatus adalah toksik kepada sel-sel pada kepekatan 100μg/ml. Kesan-kesan daripada ekstrak tumbuh-tumbuhan terhadap prokolagen I telah diuji dan didapati bahawa O. stamineus, C. hirta dan C. caudatus meningkatkan ekspresi PICP apabila diuji pada kepekatan 50, 100, 250 dan 500ng/ml. Untuk kesan perlindungan ekstrak tumbuhtumbuhan pada kepekatan 50, 100, 250 dan 500ng/ml terhadap dos sublethal hidrogen peroksida pada 750μg/ml, hanya 50ng/ml O. stamineus menunjukkan perlindungan terhadap tekanan oksidatif. Ekstrak tumbuh-tumbuhan telah disiasat selanjutnya dengan menggunakan C. elegans sebagai model organisma in vivo. The anti-aging properties of extracts from four different plants, namely O. stamineus, C. hirta, P. sarmentosum and C. caudatus were investigated. A simple extraction method was carried out before further analysis. DPPH assay indicated that ethanol extract of C. hirta showed the highest radical scavenging activities, with IC50 of 33.3 ± 1.8 μg/ml whilst ethanol extract of P. sarmentosum showed the lowest radical scavenging activities, with IC50 of 660.4 ± 14.4 μg/ml. Toxicity of plant extracts was tested on adult Human Dermal Fibroblast (HDFa). O. stamineus and C. caudatus were toxic to cells at the concentration of 100μg/ml. The effects of the plant extracts on Type I pro-collagen production were tested and it was found that O. stamineus, C. hirta and C. caudatus accelerated PICP expressions when tested at the concentrations of 50, 100, 250 and 500ng/ml. For the cytoprotection effects of plant extracts at the concentrations of 50, 100, 250 and 500ng/ml towards the sub-lethal dose of hydrogen peroxide at 750μg/ml, only 50ng/ml of O. stamineus showed protection against the oxidative stress. Plant extracts were further investigated by using C. elegans as the model in vivo organism. 2015-10 Thesis http://eprints.usm.my/31961/ http://eprints.usm.my/31961/1/LIM_YING_YU_24%28NN%29.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Farmasi (School of Pharmaceutical Sciences)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RS1-441 Pharmacy and materia medica
spellingShingle	RS1-441 Pharmacy and materia medica Lim, Ying Yu Evaluation Of The Anti-Oxidant And Anti-Aging Properties Of Extracts From Four Types Of Plants
description	Ciri-ciri anti-penuaan ekstrak daripada empat jenis tumbuh-tumbuhan yang berbeza, iaitu O. stamineus, C. hirta, P. sarmentosum dan C. caudatus telah disiasat. Kaedah pengekstrakan yang mudah telah dijalankan sebelum analisis yang seterusnya. Asai DPPH menunjukkan bahawa ekstrak etanol C. hirta menunjukkan aktiviti memerangkap radikal yang tertinggi, dengan IC50 33.3±1.8μg/ml manakala ekstrak etanol P. sarmentosum menunjukkan aktiviti memerangkap radikal yang terendah, dengan IC50 660.4±14.4μg/ml. Toksisiti ekstrak tumbuh-tumbuhan telah diuji dengan menggunakan sel-sel fibroblast kulit manusia dewasa. O. stamineus dan C. caudatus adalah toksik kepada sel-sel pada kepekatan 100μg/ml. Kesan-kesan daripada ekstrak tumbuh-tumbuhan terhadap prokolagen I telah diuji dan didapati bahawa O. stamineus, C. hirta dan C. caudatus meningkatkan ekspresi PICP apabila diuji pada kepekatan 50, 100, 250 dan 500ng/ml. Untuk kesan perlindungan ekstrak tumbuhtumbuhan pada kepekatan 50, 100, 250 dan 500ng/ml terhadap dos sublethal hidrogen peroksida pada 750μg/ml, hanya 50ng/ml O. stamineus menunjukkan perlindungan terhadap tekanan oksidatif. Ekstrak tumbuh-tumbuhan telah disiasat selanjutnya dengan menggunakan C. elegans sebagai model organisma in vivo. The anti-aging properties of extracts from four different plants, namely O. stamineus, C. hirta, P. sarmentosum and C. caudatus were investigated. A simple extraction method was carried out before further analysis. DPPH assay indicated that ethanol extract of C. hirta showed the highest radical scavenging activities, with IC50 of 33.3 ± 1.8 μg/ml whilst ethanol extract of P. sarmentosum showed the lowest radical scavenging activities, with IC50 of 660.4 ± 14.4 μg/ml. Toxicity of plant extracts was tested on adult Human Dermal Fibroblast (HDFa). O. stamineus and C. caudatus were toxic to cells at the concentration of 100μg/ml. The effects of the plant extracts on Type I pro-collagen production were tested and it was found that O. stamineus, C. hirta and C. caudatus accelerated PICP expressions when tested at the concentrations of 50, 100, 250 and 500ng/ml. For the cytoprotection effects of plant extracts at the concentrations of 50, 100, 250 and 500ng/ml towards the sub-lethal dose of hydrogen peroxide at 750μg/ml, only 50ng/ml of O. stamineus showed protection against the oxidative stress. Plant extracts were further investigated by using C. elegans as the model in vivo organism.
format	Thesis
qualification_level	Master's degree
author	Lim, Ying Yu
author_facet	Lim, Ying Yu
author_sort	Lim, Ying Yu
title	Evaluation Of The Anti-Oxidant And Anti-Aging Properties Of Extracts From Four Types Of Plants
title_short	Evaluation Of The Anti-Oxidant And Anti-Aging Properties Of Extracts From Four Types Of Plants
title_full	Evaluation Of The Anti-Oxidant And Anti-Aging Properties Of Extracts From Four Types Of Plants
title_fullStr	Evaluation Of The Anti-Oxidant And Anti-Aging Properties Of Extracts From Four Types Of Plants
title_full_unstemmed	Evaluation Of The Anti-Oxidant And Anti-Aging Properties Of Extracts From Four Types Of Plants
title_sort	evaluation of the anti-oxidant and anti-aging properties of extracts from four types of plants
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Farmasi (School of Pharmaceutical Sciences)
publishDate	2015
url	http://eprints.usm.my/31961/1/LIM_YING_YU_24%28NN%29.pdf
_version_	1747820510028234752

Evaluation Of The Anti-Oxidant And Anti-Aging Properties Of Extracts From Four Types Of Plants

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