Effects Of Oxidized Low-Density Lipoprotein By Thymoquinone In Lipidloaded Mcf-7 Breast Cancer Cells

Kanser payudara adalah punca utama kematian di kalangan wanita di seluruh dunia dan kajian baru-baru ini mendedahkan bahawa lipoprotein berketumpatan rendah teroksida (ox-LDL) menyumbang kepada peningkatan risiko kanser payudara. Thymoquinone (TQ) telah dikaji secara meluas dalam patogenesis kans...

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Main Author:	Abdul Hamid, Auni Fatin
Format:	Thesis
Language:	English
Published:	2016
Subjects:	RK1-715 Dentistry
Online Access:	http://eprints.usm.my/32109/1/AUNI_FATIN_BT_ABDUL_HAMID_24%28NN%29.pdf
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id	my-usm-ep.32109
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spelling	my-usm-ep.321092019-04-12T05:25:17Z Effects Of Oxidized Low-Density Lipoprotein By Thymoquinone In Lipidloaded Mcf-7 Breast Cancer Cells 2016-07 Abdul Hamid, Auni Fatin RK1-715 Dentistry Kanser payudara adalah punca utama kematian di kalangan wanita di seluruh dunia dan kajian baru-baru ini mendedahkan bahawa lipoprotein berketumpatan rendah teroksida (ox-LDL) menyumbang kepada peningkatan risiko kanser payudara. Thymoquinone (TQ) telah dikaji secara meluas dalam patogenesis kanser melalui in vivo dan in vitro, tetapi terdapat kekurangan laporan mengenai kawal atur lipid dalam sel kanser. Oleh itu, objektif kajian ini adalah untuk menjelaskan kesan ox-LDL kepada pertumbuhan sel kanser payudara serta mekanisma metabolisma lipid dan pertumbuhan sel di peringkat molekul yang dikawal atur oleh TQ. Kesan toksik TQ dianalisis melalui ujian MTS. Sel MCF-7 didedahkan kepada 10 μg/ml ox-LDL, diikuti oleh rawatan dengan 20 μM TQ untuk kajian ke atas kebergantungan kepada masa. Lokasi dan ekspresi protein sasaran dikaji melalui kaedah immunopendarfluor dan pemendapan Western. Ini diikuti oleh analisis qRT-PCR ke atas ekspresi VAMP4 dan EGLN1. Breast cancer is the leading cause of death among women worldwide and recent studies revealed that oxidized low-density lipoprotein (ox-LDL) contributed to increased risk of breast cancer. To date, thymoquinone (TQ) is widely studied both in vivo and in vitro in cancer pathogenesis, but there is lack of reports on its lipid regulation in cancer cells. Thus, the objectives of this study were to elucidate the effects of ox-LDL on breast cancer cell growth as well as the molecular mechanisms of lipid metabolism and cell proliferation regulated by TQ. Cytotoxicity of TQ was analyzed using MTS assay. MCF-7 cells were exposed to 10 μg/ml of ox-LDL, followed by treatment with 20 μM of TQ for time-dependent study. Localization and expression of target proteins were studied through immunofluorescence and Western blot methods. This was followed by qRT-PCR analysis on genomic expression of VAMP4 and EGLN1. 2016-07 Thesis http://eprints.usm.my/32109/ http://eprints.usm.my/32109/1/AUNI_FATIN_BT_ABDUL_HAMID_24%28NN%29.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RK1-715 Dentistry
spellingShingle	RK1-715 Dentistry Abdul Hamid, Auni Fatin Effects Of Oxidized Low-Density Lipoprotein By Thymoquinone In Lipidloaded Mcf-7 Breast Cancer Cells
description	Kanser payudara adalah punca utama kematian di kalangan wanita di seluruh dunia dan kajian baru-baru ini mendedahkan bahawa lipoprotein berketumpatan rendah teroksida (ox-LDL) menyumbang kepada peningkatan risiko kanser payudara. Thymoquinone (TQ) telah dikaji secara meluas dalam patogenesis kanser melalui in vivo dan in vitro, tetapi terdapat kekurangan laporan mengenai kawal atur lipid dalam sel kanser. Oleh itu, objektif kajian ini adalah untuk menjelaskan kesan ox-LDL kepada pertumbuhan sel kanser payudara serta mekanisma metabolisma lipid dan pertumbuhan sel di peringkat molekul yang dikawal atur oleh TQ. Kesan toksik TQ dianalisis melalui ujian MTS. Sel MCF-7 didedahkan kepada 10 μg/ml ox-LDL, diikuti oleh rawatan dengan 20 μM TQ untuk kajian ke atas kebergantungan kepada masa. Lokasi dan ekspresi protein sasaran dikaji melalui kaedah immunopendarfluor dan pemendapan Western. Ini diikuti oleh analisis qRT-PCR ke atas ekspresi VAMP4 dan EGLN1. Breast cancer is the leading cause of death among women worldwide and recent studies revealed that oxidized low-density lipoprotein (ox-LDL) contributed to increased risk of breast cancer. To date, thymoquinone (TQ) is widely studied both in vivo and in vitro in cancer pathogenesis, but there is lack of reports on its lipid regulation in cancer cells. Thus, the objectives of this study were to elucidate the effects of ox-LDL on breast cancer cell growth as well as the molecular mechanisms of lipid metabolism and cell proliferation regulated by TQ. Cytotoxicity of TQ was analyzed using MTS assay. MCF-7 cells were exposed to 10 μg/ml of ox-LDL, followed by treatment with 20 μM of TQ for time-dependent study. Localization and expression of target proteins were studied through immunofluorescence and Western blot methods. This was followed by qRT-PCR analysis on genomic expression of VAMP4 and EGLN1.
format	Thesis
qualification_level	Master's degree
author	Abdul Hamid, Auni Fatin
author_facet	Abdul Hamid, Auni Fatin
author_sort	Abdul Hamid, Auni Fatin
title	Effects Of Oxidized Low-Density Lipoprotein By Thymoquinone In Lipidloaded Mcf-7 Breast Cancer Cells
title_short	Effects Of Oxidized Low-Density Lipoprotein By Thymoquinone In Lipidloaded Mcf-7 Breast Cancer Cells
title_full	Effects Of Oxidized Low-Density Lipoprotein By Thymoquinone In Lipidloaded Mcf-7 Breast Cancer Cells
title_fullStr	Effects Of Oxidized Low-Density Lipoprotein By Thymoquinone In Lipidloaded Mcf-7 Breast Cancer Cells
title_full_unstemmed	Effects Of Oxidized Low-Density Lipoprotein By Thymoquinone In Lipidloaded Mcf-7 Breast Cancer Cells
title_sort	effects of oxidized low-density lipoprotein by thymoquinone in lipidloaded mcf-7 breast cancer cells
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute)
publishDate	2016
url	http://eprints.usm.my/32109/1/AUNI_FATIN_BT_ABDUL_HAMID_24%28NN%29.pdf
_version_	1747820529919721472

Effects Of Oxidized Low-Density Lipoprotein By Thymoquinone In Lipidloaded Mcf-7 Breast Cancer Cells

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