Isolation Of Chavibetol And Hydroxychavicol And Selected Anti-Obesity Studies Of Standardized Piper Betle Linn. Leaf Extracts

Piper betle L. telah digunakan secara meluas dalam perubatan herba tradisional di Asia. Walau bagaimanapun, tumbuhan ini masih kekurangan data analisis terutama berkaitan kualiti, keselamatan dan keberkesanannya. Kajian ini telah dijalankan untuk menyediakan maklumat mengenai pemiawaian daun Piper b...

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Main Author:	Mohamad, Nurul Najwa
Format:	Thesis
Language:	English
Published:	2016
Subjects:	RS1-441 Pharmacy and materia medica
Online Access:	http://eprints.usm.my/32243/1/NURUL_NAJWA_MOHAMAD_24%28NN%29.pdf
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id	my-usm-ep.32243
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spelling	my-usm-ep.322432019-04-12T05:25:14Z Isolation Of Chavibetol And Hydroxychavicol And Selected Anti-Obesity Studies Of Standardized Piper Betle Linn. Leaf Extracts 2016-09 Mohamad, Nurul Najwa RS1-441 Pharmacy and materia medica Piper betle L. telah digunakan secara meluas dalam perubatan herba tradisional di Asia. Walau bagaimanapun, tumbuhan ini masih kekurangan data analisis terutama berkaitan kualiti, keselamatan dan keberkesanannya. Kajian ini telah dijalankan untuk menyediakan maklumat mengenai pemiawaian daun Piper betle. Kajian ini dibahagikan kepada tiga bahagian: kualiti dan keselamatan bahan-bahan mentah daun Piper betle, piawaian ekstrak Piper betle menggunakan sebatian terasing dan anti-obesiti. Daun kering Piper betle ditentukan untuk kandungan logam berat dan kandungan ujian had mikrob (MLT). Tumbuhan ini didapati berada dalam lingkungan had yang ditetapkan bagi empat logam berat Cd (0.3ppm), Hg (0.5 ppm), Pb (10 ppm) and As (5.0 ppm) dan ia bebas dari pencemaran mikrob. Pemeriksaan fitokimia awal bagi ekstrak etanol, ekstrak 50% etanol dan ekstrak air menunjukkan kehadiran sebatian terpenoid, flavonoid, fenol, sterol, koumarin dan saponin. Dua sebatian iaitu kavibetol (CHV) dan hidroksikavikol (HC) telah diasingkan dan dikenalpasti melalui data UV, FTIR, TLC, HPLC, GC-MS dan NMR. Profil kimia yang menggunakan teknik spektroskopi dan kromatografi seperti UV, FTIR, HPTLC dan GC-MS digunakan dalam kajian pemiawaian. Piper betle L. has been extensively used in traditional herbal remedies in Asia. Nevertheless, this plant still lacks of data especially on their quality, safety and efficacy. This study has been conducted to provide information on the standardization of Piper betle leaves. The study was divided into three parts: quality and safety of raw materials of Piper betle leaves, standardization of Piper betle extracts using isolation compounds and anti-obesity studies. Dry powdered leaves of Piper betle were determined to evaluate the content of heavy metals and the microbial limit test (MLT). This plant was found to be in the range of the acceptable limit of four main heavy metals Cd (0.3ppm), Hg (0.5 ppm), Pb (10 ppm) and As (5.0 ppm) and free from microbial contamination. The preliminary phytochemical screening using TLC on the ethanol, 50% ethanol and water extracts showed the presence of terpenoids, flavonoids, phenols, sterols, coumarins and saponins. Two compounds namely chavibetol (CHV) and hydroxychavicol (HC) were isolated and identified by UV, FTIR, HPTLC, HPLC, GC-MS and NMR data. The chemical profile using spectroscopic and chromatographic techniques such as UV, FTIR, HPTLC and GC-MS were used in the standardization. 2016-09 Thesis http://eprints.usm.my/32243/ http://eprints.usm.my/32243/1/NURUL_NAJWA_MOHAMAD_24%28NN%29.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Farmasi (School of Pharmaceutical Sciences)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RS1-441 Pharmacy and materia medica
spellingShingle	RS1-441 Pharmacy and materia medica Mohamad, Nurul Najwa Isolation Of Chavibetol And Hydroxychavicol And Selected Anti-Obesity Studies Of Standardized Piper Betle Linn. Leaf Extracts
description	Piper betle L. telah digunakan secara meluas dalam perubatan herba tradisional di Asia. Walau bagaimanapun, tumbuhan ini masih kekurangan data analisis terutama berkaitan kualiti, keselamatan dan keberkesanannya. Kajian ini telah dijalankan untuk menyediakan maklumat mengenai pemiawaian daun Piper betle. Kajian ini dibahagikan kepada tiga bahagian: kualiti dan keselamatan bahan-bahan mentah daun Piper betle, piawaian ekstrak Piper betle menggunakan sebatian terasing dan anti-obesiti. Daun kering Piper betle ditentukan untuk kandungan logam berat dan kandungan ujian had mikrob (MLT). Tumbuhan ini didapati berada dalam lingkungan had yang ditetapkan bagi empat logam berat Cd (0.3ppm), Hg (0.5 ppm), Pb (10 ppm) and As (5.0 ppm) dan ia bebas dari pencemaran mikrob. Pemeriksaan fitokimia awal bagi ekstrak etanol, ekstrak 50% etanol dan ekstrak air menunjukkan kehadiran sebatian terpenoid, flavonoid, fenol, sterol, koumarin dan saponin. Dua sebatian iaitu kavibetol (CHV) dan hidroksikavikol (HC) telah diasingkan dan dikenalpasti melalui data UV, FTIR, TLC, HPLC, GC-MS dan NMR. Profil kimia yang menggunakan teknik spektroskopi dan kromatografi seperti UV, FTIR, HPTLC dan GC-MS digunakan dalam kajian pemiawaian. Piper betle L. has been extensively used in traditional herbal remedies in Asia. Nevertheless, this plant still lacks of data especially on their quality, safety and efficacy. This study has been conducted to provide information on the standardization of Piper betle leaves. The study was divided into three parts: quality and safety of raw materials of Piper betle leaves, standardization of Piper betle extracts using isolation compounds and anti-obesity studies. Dry powdered leaves of Piper betle were determined to evaluate the content of heavy metals and the microbial limit test (MLT). This plant was found to be in the range of the acceptable limit of four main heavy metals Cd (0.3ppm), Hg (0.5 ppm), Pb (10 ppm) and As (5.0 ppm) and free from microbial contamination. The preliminary phytochemical screening using TLC on the ethanol, 50% ethanol and water extracts showed the presence of terpenoids, flavonoids, phenols, sterols, coumarins and saponins. Two compounds namely chavibetol (CHV) and hydroxychavicol (HC) were isolated and identified by UV, FTIR, HPTLC, HPLC, GC-MS and NMR data. The chemical profile using spectroscopic and chromatographic techniques such as UV, FTIR, HPTLC and GC-MS were used in the standardization.
format	Thesis
qualification_level	Master's degree
author	Mohamad, Nurul Najwa
author_facet	Mohamad, Nurul Najwa
author_sort	Mohamad, Nurul Najwa
title	Isolation Of Chavibetol And Hydroxychavicol And Selected Anti-Obesity Studies Of Standardized Piper Betle Linn. Leaf Extracts
title_short	Isolation Of Chavibetol And Hydroxychavicol And Selected Anti-Obesity Studies Of Standardized Piper Betle Linn. Leaf Extracts
title_full	Isolation Of Chavibetol And Hydroxychavicol And Selected Anti-Obesity Studies Of Standardized Piper Betle Linn. Leaf Extracts
title_fullStr	Isolation Of Chavibetol And Hydroxychavicol And Selected Anti-Obesity Studies Of Standardized Piper Betle Linn. Leaf Extracts
title_full_unstemmed	Isolation Of Chavibetol And Hydroxychavicol And Selected Anti-Obesity Studies Of Standardized Piper Betle Linn. Leaf Extracts
title_sort	isolation of chavibetol and hydroxychavicol and selected anti-obesity studies of standardized piper betle linn. leaf extracts
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Farmasi (School of Pharmaceutical Sciences)
publishDate	2016
url	http://eprints.usm.my/32243/1/NURUL_NAJWA_MOHAMAD_24%28NN%29.pdf
_version_	1747820551770996736

Isolation Of Chavibetol And Hydroxychavicol And Selected Anti-Obesity Studies Of Standardized Piper Betle Linn. Leaf Extracts

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