In Vitro Investigation Of Cytotoxicity And Apoptosis Induction By Methanol Extract Of Calophyllum Inophyllum Fruit In MCF-7 Human Breast Cancer Cell Line

Calophyllum inophyllum adalah sejenis tumbuhan bakawali yang kaya dengan nilai-nilai perubatan yang telah dimanfaatkan secara meluas dari zaman dahulu lagi untuk menyembuhkan pelbagai penyakit. Walaupun C. inophyllum termahsyur dengan sejarah perubatan tradisionalnya, tetapi hanya sedikit bukti sain...

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Main Author:	Shanmugapriya, Shanmugapriya
Format:	Thesis
Language:	English
Published:	2016
Subjects:	R735-854 Medical education Medical schools Research
Online Access:	http://eprints.usm.my/32260/1/SHANMUGAPRIYA_24%28NN%29.pdf
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id	my-usm-ep.32260
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spelling	my-usm-ep.322602019-04-12T05:25:19Z In Vitro Investigation Of Cytotoxicity And Apoptosis Induction By Methanol Extract Of Calophyllum Inophyllum Fruit In MCF-7 Human Breast Cancer Cell Line 2016-07 Shanmugapriya, Shanmugapriya R735-854 Medical education. Medical schools. Research Calophyllum inophyllum adalah sejenis tumbuhan bakawali yang kaya dengan nilai-nilai perubatan yang telah dimanfaatkan secara meluas dari zaman dahulu lagi untuk menyembuhkan pelbagai penyakit. Walaupun C. inophyllum termahsyur dengan sejarah perubatan tradisionalnya, tetapi hanya sedikit bukti saintifik yang melaporkan tentang ketoksikan C. inophyllum khususnya buah C. inophylum, dimana tiada kajian langsung yang melaporkan tentang mekanisma tahap molekul yang komprehensif. Oleh itu, penyelidikan ini telah dilaksanakan untuk menentukan ketoksikan ekstrak buah C. inophyllum secara in vitro terhadap titisan sel kanser manusia MCF-7 dengan penjelasan terperinci mengenai mekanisma tahap molekular disebalik kematian sel kanser. Dalam kajian ini, pengesahan spesies tumbuhan serta analisis jumlah kandungan fenol telah dijalankan. Kesan sitotoksisiti ekstrak buah C. inophyllum terhadap titisan sel kanser MCF-7 telah dinilai dengan menjalankan ujian-ujian MTT dan CyQuant yang menunjukkan perencatan pertumbuhan sel MCF-7 dengan nilai IC50 sebanyak 19.63 μg/mL dan 27.54 μg/mL masing-masing. Penelitian terhadap morfologi sel MCF-7 setelah dirawat dengan ekstrak buah C. inophyllum pada kepekatan IC50 (23.59 μg/mL) telah memaparkan ciri-ciri histologi apoptosis yang jelas. Calophyllum inophyllum is an exquisite plant species with rich ethnomedicinal values have been diversely utilized to heal several diseases. In spite of its long-established sophisticated traditional medicinal properties, only a few investigations have reported its cytotoxicity especially fruit extract, with absolutely no means of scientific evidence of its comprehensive molecular mechanism. Hence, this study was conducted to determine the in vitro cytotoxicity of C. inophyllum fruit extract against MCF-7 human breast cancer cells with an intricate elucidation of the molecular mechanism of the cell death. In this study, authentication of the plant species and the determination of total phenolic content were carried out. The cytotoxic effect of C. inophyllum fruit extract against MCF-7 cancer cells was evaluated through MTT and CyQuant assays which demonstrated the inhibition of cell viability with the IC50 values 19.63 μg/mL and 27.54 μg/mL respectively. The preliminary time-based morphological investigation of MCF-7 cells treated with the IC50 value (23.59 μg/mL) of C. inophyllum fruit extract revealed prominent histological characteristics of apoptosis. 2016-07 Thesis http://eprints.usm.my/32260/ http://eprints.usm.my/32260/1/SHANMUGAPRIYA_24%28NN%29.pdf application/pdf en public masters Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul (INFORMM)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R735-854 Medical education Medical schools Research
spellingShingle	R735-854 Medical education Medical schools Research Shanmugapriya, Shanmugapriya In Vitro Investigation Of Cytotoxicity And Apoptosis Induction By Methanol Extract Of Calophyllum Inophyllum Fruit In MCF-7 Human Breast Cancer Cell Line
description	Calophyllum inophyllum adalah sejenis tumbuhan bakawali yang kaya dengan nilai-nilai perubatan yang telah dimanfaatkan secara meluas dari zaman dahulu lagi untuk menyembuhkan pelbagai penyakit. Walaupun C. inophyllum termahsyur dengan sejarah perubatan tradisionalnya, tetapi hanya sedikit bukti saintifik yang melaporkan tentang ketoksikan C. inophyllum khususnya buah C. inophylum, dimana tiada kajian langsung yang melaporkan tentang mekanisma tahap molekul yang komprehensif. Oleh itu, penyelidikan ini telah dilaksanakan untuk menentukan ketoksikan ekstrak buah C. inophyllum secara in vitro terhadap titisan sel kanser manusia MCF-7 dengan penjelasan terperinci mengenai mekanisma tahap molekular disebalik kematian sel kanser. Dalam kajian ini, pengesahan spesies tumbuhan serta analisis jumlah kandungan fenol telah dijalankan. Kesan sitotoksisiti ekstrak buah C. inophyllum terhadap titisan sel kanser MCF-7 telah dinilai dengan menjalankan ujian-ujian MTT dan CyQuant yang menunjukkan perencatan pertumbuhan sel MCF-7 dengan nilai IC50 sebanyak 19.63 μg/mL dan 27.54 μg/mL masing-masing. Penelitian terhadap morfologi sel MCF-7 setelah dirawat dengan ekstrak buah C. inophyllum pada kepekatan IC50 (23.59 μg/mL) telah memaparkan ciri-ciri histologi apoptosis yang jelas. Calophyllum inophyllum is an exquisite plant species with rich ethnomedicinal values have been diversely utilized to heal several diseases. In spite of its long-established sophisticated traditional medicinal properties, only a few investigations have reported its cytotoxicity especially fruit extract, with absolutely no means of scientific evidence of its comprehensive molecular mechanism. Hence, this study was conducted to determine the in vitro cytotoxicity of C. inophyllum fruit extract against MCF-7 human breast cancer cells with an intricate elucidation of the molecular mechanism of the cell death. In this study, authentication of the plant species and the determination of total phenolic content were carried out. The cytotoxic effect of C. inophyllum fruit extract against MCF-7 cancer cells was evaluated through MTT and CyQuant assays which demonstrated the inhibition of cell viability with the IC50 values 19.63 μg/mL and 27.54 μg/mL respectively. The preliminary time-based morphological investigation of MCF-7 cells treated with the IC50 value (23.59 μg/mL) of C. inophyllum fruit extract revealed prominent histological characteristics of apoptosis.
format	Thesis
qualification_level	Master's degree
author	Shanmugapriya, Shanmugapriya
author_facet	Shanmugapriya, Shanmugapriya
author_sort	Shanmugapriya, Shanmugapriya
title	In Vitro Investigation Of Cytotoxicity And Apoptosis Induction By Methanol Extract Of Calophyllum Inophyllum Fruit In MCF-7 Human Breast Cancer Cell Line
title_short	In Vitro Investigation Of Cytotoxicity And Apoptosis Induction By Methanol Extract Of Calophyllum Inophyllum Fruit In MCF-7 Human Breast Cancer Cell Line
title_full	In Vitro Investigation Of Cytotoxicity And Apoptosis Induction By Methanol Extract Of Calophyllum Inophyllum Fruit In MCF-7 Human Breast Cancer Cell Line
title_fullStr	In Vitro Investigation Of Cytotoxicity And Apoptosis Induction By Methanol Extract Of Calophyllum Inophyllum Fruit In MCF-7 Human Breast Cancer Cell Line
title_full_unstemmed	In Vitro Investigation Of Cytotoxicity And Apoptosis Induction By Methanol Extract Of Calophyllum Inophyllum Fruit In MCF-7 Human Breast Cancer Cell Line
title_sort	in vitro investigation of cytotoxicity and apoptosis induction by methanol extract of calophyllum inophyllum fruit in mcf-7 human breast cancer cell line
granting_institution	Universiti Sains Malaysia
granting_department	Institut Penyelidikan Perubatan Molekul (INFORMM)
publishDate	2016
url	http://eprints.usm.my/32260/1/SHANMUGAPRIYA_24%28NN%29.pdf
_version_	1747820554724835328

In Vitro Investigation Of Cytotoxicity And Apoptosis Induction By Methanol Extract Of Calophyllum Inophyllum Fruit In MCF-7 Human Breast Cancer Cell Line

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