Desaturase Delta-6 mRNA Expressions In Highly Unsaturated Fatty Acids Biosynthesis Pathway During Follicle Maturation In Female Zebrafish (Danio rerio)

Sangat sedikit yang diketahui tentang sintesis asid lemak tidak tepu seperti asid eikosapentanoik (EPA, C20:5n-3), asid dokosaheksaenoik acid (DHA, C22:6n- 3) dan asid arakidonik (ARA, C20:4n-6), atau secara berkumpulan lebih dikenali sebagai asid lemak sangat tidak tepu (HUF A), di dalam ovari i...

Full description

Saved in:

Bibliographic Details
Main Author:	Ishak, Sairatul Dahlianis
Format:	Thesis
Language:	English
Published:	2010
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/32377/1/SAIRA_TUL_DAHLIANIS_ISHAK_24%28NN%29.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.32377
record_format	uketd_dc
spelling	my-usm-ep.323772019-04-12T05:26:51Z Desaturase Delta-6 mRNA Expressions In Highly Unsaturated Fatty Acids Biosynthesis Pathway During Follicle Maturation In Female Zebrafish (Danio rerio) 2010-06 Ishak, Sairatul Dahlianis QH1 Natural history (General - Including nature conservation, geographical distribution) Sangat sedikit yang diketahui tentang sintesis asid lemak tidak tepu seperti asid eikosapentanoik (EPA, C20:5n-3), asid dokosaheksaenoik acid (DHA, C22:6n- 3) dan asid arakidonik (ARA, C20:4n-6), atau secara berkumpulan lebih dikenali sebagai asid lemak sangat tidak tepu (HUF A), di dalam ovari ikan walaupun ia memainkan peranan yang sangat penting dalam pembiakan vertebrata ini. Ikan zebrafish (Dania rerio) mempamerkan keupayaan untuk mensintesis ketiga-tiga HUF A ini melalui kitaran yang melibatkan desaturasi dan elongasi dua prekursor iaitu asid linoleik (LA, Cl8:2n-6) dan asid linolenik (LNA, Cl8:3n-3). Bagi lebih memahami kepentingan serta pengawalaturan sintesis HUF A dalam ovari, kaj ian ini menunjukkan corak ekspresi mRNA untuk desaturase (jadsd6), iaitu satu enzim perlu yang terlibat dalam kitaran biosintesis HUF A dalam lima peringkat folikel ovari zebrafish yang berbeza iaitu pre-vitelogenik, vitelogenik awal, vitelogenik akhir, kematangan dan ovulasi. Aras mRNA bagi fadsd6 dalam peringkat-peringkat folikel ovari yang berbeza ini ditentukan oleh kaedah esei RT-PCR. Very little is known about the synthesis of unsaturated fatty acids such as eicosapentaenoic acid (EPA, C20:5n-3), docosahexaenoic acid (DHA, C22:6n-3) and arachidonic acid (ARA, C20:4n-6), or collectively known as the highly unsaturated fatty acids (HUF A), in the fish ovary although they play pivotal roles in this vertebrate reproduction. The zebrafish (Dania rerio) display capability to synthesize all three HUF A via pathways involving desaturation and elongation of two precursors, the linoleic acid (LA, C18:2n-6) and linolenic acid (LNA, Cl8:3n-3). In order to gain understanding on the importance and regulation of ovarian HUF A synthesis, this study shows the mRNA expression pattern of desaturase (jadsd6), an essential enzyme involved in HUF A biosynthesis pathway, in five different zebrafish ovarian follicle stages; pre-vitellogenic, early vitellogenic, late vitellogenic, maturation and ovulation stages. The mRNA levels of fadsd6 in different ovarian follicle stages were determined by semi-quantitative RT-PCR assays. 2010-06 Thesis http://eprints.usm.my/32377/ http://eprints.usm.my/32377/1/SAIRA_TUL_DAHLIANIS_ISHAK_24%28NN%29.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Ishak, Sairatul Dahlianis Desaturase Delta-6 mRNA Expressions In Highly Unsaturated Fatty Acids Biosynthesis Pathway During Follicle Maturation In Female Zebrafish (Danio rerio)
description	Sangat sedikit yang diketahui tentang sintesis asid lemak tidak tepu seperti asid eikosapentanoik (EPA, C20:5n-3), asid dokosaheksaenoik acid (DHA, C22:6n- 3) dan asid arakidonik (ARA, C20:4n-6), atau secara berkumpulan lebih dikenali sebagai asid lemak sangat tidak tepu (HUF A), di dalam ovari ikan walaupun ia memainkan peranan yang sangat penting dalam pembiakan vertebrata ini. Ikan zebrafish (Dania rerio) mempamerkan keupayaan untuk mensintesis ketiga-tiga HUF A ini melalui kitaran yang melibatkan desaturasi dan elongasi dua prekursor iaitu asid linoleik (LA, Cl8:2n-6) dan asid linolenik (LNA, Cl8:3n-3). Bagi lebih memahami kepentingan serta pengawalaturan sintesis HUF A dalam ovari, kaj ian ini menunjukkan corak ekspresi mRNA untuk desaturase (jadsd6), iaitu satu enzim perlu yang terlibat dalam kitaran biosintesis HUF A dalam lima peringkat folikel ovari zebrafish yang berbeza iaitu pre-vitelogenik, vitelogenik awal, vitelogenik akhir, kematangan dan ovulasi. Aras mRNA bagi fadsd6 dalam peringkat-peringkat folikel ovari yang berbeza ini ditentukan oleh kaedah esei RT-PCR. Very little is known about the synthesis of unsaturated fatty acids such as eicosapentaenoic acid (EPA, C20:5n-3), docosahexaenoic acid (DHA, C22:6n-3) and arachidonic acid (ARA, C20:4n-6), or collectively known as the highly unsaturated fatty acids (HUF A), in the fish ovary although they play pivotal roles in this vertebrate reproduction. The zebrafish (Dania rerio) display capability to synthesize all three HUF A via pathways involving desaturation and elongation of two precursors, the linoleic acid (LA, C18:2n-6) and linolenic acid (LNA, Cl8:3n-3). In order to gain understanding on the importance and regulation of ovarian HUF A synthesis, this study shows the mRNA expression pattern of desaturase (jadsd6), an essential enzyme involved in HUF A biosynthesis pathway, in five different zebrafish ovarian follicle stages; pre-vitellogenic, early vitellogenic, late vitellogenic, maturation and ovulation stages. The mRNA levels of fadsd6 in different ovarian follicle stages were determined by semi-quantitative RT-PCR assays.
format	Thesis
qualification_level	Master's degree
author	Ishak, Sairatul Dahlianis
author_facet	Ishak, Sairatul Dahlianis
author_sort	Ishak, Sairatul Dahlianis
title	Desaturase Delta-6 mRNA Expressions In Highly Unsaturated Fatty Acids Biosynthesis Pathway During Follicle Maturation In Female Zebrafish (Danio rerio)
title_short	Desaturase Delta-6 mRNA Expressions In Highly Unsaturated Fatty Acids Biosynthesis Pathway During Follicle Maturation In Female Zebrafish (Danio rerio)
title_full	Desaturase Delta-6 mRNA Expressions In Highly Unsaturated Fatty Acids Biosynthesis Pathway During Follicle Maturation In Female Zebrafish (Danio rerio)
title_fullStr	Desaturase Delta-6 mRNA Expressions In Highly Unsaturated Fatty Acids Biosynthesis Pathway During Follicle Maturation In Female Zebrafish (Danio rerio)
title_full_unstemmed	Desaturase Delta-6 mRNA Expressions In Highly Unsaturated Fatty Acids Biosynthesis Pathway During Follicle Maturation In Female Zebrafish (Danio rerio)
title_sort	desaturase delta-6 mrna expressions in highly unsaturated fatty acids biosynthesis pathway during follicle maturation in female zebrafish (danio rerio)
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
publishDate	2010
url	http://eprints.usm.my/32377/1/SAIRA_TUL_DAHLIANIS_ISHAK_24%28NN%29.pdf
_version_	1747820570419920896

Desaturase Delta-6 mRNA Expressions In Highly Unsaturated Fatty Acids Biosynthesis Pathway During Follicle Maturation In Female Zebrafish (Danio rerio)

Similar Items