Bioassay Guided Isolation Of Antitermite Compounds From The Bark Of Rhizophora Apiculata

Serbuk kulit kayu Rhizophora apiculata pada mulanya diekstrak dengan petroleum eter (PE) dan metanol. Ekstrak metanol dalam bentuk larutan akueus telah diekstrak semula ke dalam pecahan kloroform (CE), etil asetat (EE) dan n-butanol (BE). Pengasingan berpandukan ujian makmal (ASTM D3345-74) telah...

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Main Author:	Kong , Nein Hing
Format:	Thesis
Language:	English
Published:	2009
Subjects:	QD1-999 Chemistry
Online Access:	http://eprints.usm.my/41274/1/Kong_Nein_Hing.pdf
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spelling	my-usm-ep.412742019-04-12T05:27:00Z Bioassay Guided Isolation Of Antitermite Compounds From The Bark Of Rhizophora Apiculata 2009-11 Kong , Nein Hing QD1-999 Chemistry Serbuk kulit kayu Rhizophora apiculata pada mulanya diekstrak dengan petroleum eter (PE) dan metanol. Ekstrak metanol dalam bentuk larutan akueus telah diekstrak semula ke dalam pecahan kloroform (CE), etil asetat (EE) dan n-butanol (BE). Pengasingan berpandukan ujian makmal (ASTM D3345-74) telah dijalankan dengan mempertimbangkan kehilangan berat kayu minimum (LWL). Aktiviti anti anai-anainya (ATA) adalah EE>CE>PE>BE, LWLEE = 10.54% pada 500 ppm. Secara perbandingan, kawalan telah haus dimakan. Fraksi-fraksi EE menunjukkan ATA dalam turutan F2>F3>F1, LWLF2 = 5.53%. Analisis kimia dan spektroskopi menentukan asid fenolik adalah komponen aktif dalam EE. Fraksi F2 seterusnya diekstrak dengan kaedah asid-bes (pH ~3) dan hasilnya dikromatografkan. Sifat-sifat kimia dan biologi tidak berubah selepas pemisahan dan ATAnya adalah F2AII>F2AIII>(F2R≈F2AI), LWLF2AII, F2AIII = 6.86, 9.12%. The powdered bark of Rhizophora apiculata was initially extracted with petroleum ether (PE) and methanol. The aqueous methanol extract was re-extracted into chloroform (CE), ethyl acetate (EE) and n-butanol (BE). Bioassay (ASTM D3345-74) guided fractionation was carried out on the basis of the least wood loss (LWL). The anti-termite activity (ATA) was in the order of EE>CE>PE>BE, LWLEE = 10.54% at 500 ppm. In contrast, controls were severely damaged. Fractions of EE showed ATA in the order of F2>F3>F1, LWLF2 = 5.53%. Chemical and spectral studies proposed that the active compounds in EE were of phenolic acids. Fraction F2 was then partitioned into aqueous and organic layers at pH ~3 and the organic residue was subjected to chromatographic separation. The chemical and biological properties of the F2 fractions were not altered and the ATA sequence was F2AII>F2AIII>(F2R≈F2AI), LWLF2AII, F2AIII = 6.86, 9.12%. 2009-11 Thesis http://eprints.usm.my/41274/ http://eprints.usm.my/41274/1/Kong_Nein_Hing.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kimia
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QD1-999 Chemistry
spellingShingle	QD1-999 Chemistry Kong , Nein Hing Bioassay Guided Isolation Of Antitermite Compounds From The Bark Of Rhizophora Apiculata
description	Serbuk kulit kayu Rhizophora apiculata pada mulanya diekstrak dengan petroleum eter (PE) dan metanol. Ekstrak metanol dalam bentuk larutan akueus telah diekstrak semula ke dalam pecahan kloroform (CE), etil asetat (EE) dan n-butanol (BE). Pengasingan berpandukan ujian makmal (ASTM D3345-74) telah dijalankan dengan mempertimbangkan kehilangan berat kayu minimum (LWL). Aktiviti anti anai-anainya (ATA) adalah EE>CE>PE>BE, LWLEE = 10.54% pada 500 ppm. Secara perbandingan, kawalan telah haus dimakan. Fraksi-fraksi EE menunjukkan ATA dalam turutan F2>F3>F1, LWLF2 = 5.53%. Analisis kimia dan spektroskopi menentukan asid fenolik adalah komponen aktif dalam EE. Fraksi F2 seterusnya diekstrak dengan kaedah asid-bes (pH ~3) dan hasilnya dikromatografkan. Sifat-sifat kimia dan biologi tidak berubah selepas pemisahan dan ATAnya adalah F2AII>F2AIII>(F2R≈F2AI), LWLF2AII, F2AIII = 6.86, 9.12%. The powdered bark of Rhizophora apiculata was initially extracted with petroleum ether (PE) and methanol. The aqueous methanol extract was re-extracted into chloroform (CE), ethyl acetate (EE) and n-butanol (BE). Bioassay (ASTM D3345-74) guided fractionation was carried out on the basis of the least wood loss (LWL). The anti-termite activity (ATA) was in the order of EE>CE>PE>BE, LWLEE = 10.54% at 500 ppm. In contrast, controls were severely damaged. Fractions of EE showed ATA in the order of F2>F3>F1, LWLF2 = 5.53%. Chemical and spectral studies proposed that the active compounds in EE were of phenolic acids. Fraction F2 was then partitioned into aqueous and organic layers at pH ~3 and the organic residue was subjected to chromatographic separation. The chemical and biological properties of the F2 fractions were not altered and the ATA sequence was F2AII>F2AIII>(F2R≈F2AI), LWLF2AII, F2AIII = 6.86, 9.12%.
format	Thesis
qualification_level	Master's degree
author	Kong , Nein Hing
author_facet	Kong , Nein Hing
author_sort	Kong , Nein Hing
title	Bioassay Guided Isolation Of Antitermite Compounds From The Bark Of Rhizophora Apiculata
title_short	Bioassay Guided Isolation Of Antitermite Compounds From The Bark Of Rhizophora Apiculata
title_full	Bioassay Guided Isolation Of Antitermite Compounds From The Bark Of Rhizophora Apiculata
title_fullStr	Bioassay Guided Isolation Of Antitermite Compounds From The Bark Of Rhizophora Apiculata
title_full_unstemmed	Bioassay Guided Isolation Of Antitermite Compounds From The Bark Of Rhizophora Apiculata
title_sort	bioassay guided isolation of antitermite compounds from the bark of rhizophora apiculata
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kimia
publishDate	2009
url	http://eprints.usm.my/41274/1/Kong_Nein_Hing.pdf
_version_	1747820899824828416

Bioassay Guided Isolation Of Antitermite Compounds From The Bark Of Rhizophora Apiculata

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