The relationship of P53 gene mutation with clinicopathological characteristic in breast cancer

Background: P53 is a tumour suppresor gene. In breast cancer, p53 gene mutation were noted with frequency of about 30% (range 15 to 71%) and associated with poor prognosis. This study was perform determine p53 mutation association with clinicopathological characteristic in breast cancer and to asses...

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Main Author:	Amrank, Fitreena Anis
Format:	Thesis
Language:	English
Published:	2016
Subjects:	RC0321 Neuroscience Biological psychiatry Neuropsychiatry
Online Access:	http://eprints.usm.my/41862/1/Dr._Fitreena_Anis-24_pages.pdf
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spelling	my-usm-ep.418622019-04-12T05:25:28Z The relationship of P53 gene mutation with clinicopathological characteristic in breast cancer 2016 Amrank, Fitreena Anis RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry Background: P53 is a tumour suppresor gene. In breast cancer, p53 gene mutation were noted with frequency of about 30% (range 15 to 71%) and associated with poor prognosis. This study was perform determine p53 mutation association with clinicopathological characteristic in breast cancer and to assess the suitability of patients’ serum to detect p53 autoantibody. Methods: This study conducted in Hospital Seberang Jaya and Institut Perubatan dan Pergigian Termaju, Universiti Sains Malaysia. Six four breast cancer patients with available fresh breast cancer tissue that been kept under -80°C and with complete clinicopathological data involve in this study. These fresh breast tissues DNA extracted and 10sampels sent for DNA sequencing. The remaining 54samples proceeded with Polymerase Chains Reaction analysis based on the result from DNA sequencing. The serum of these patients was also taken for p53 autoantibody study using ELISA method. Results: The mean age of the patients in this study was 52.45±9.51 years. Most of the patients were Malay with 67.2% followed by Indian and Chinese with 17.2% and 15.6% respectively. About 51.6% of these patients undergone CT scan staging and 14.1% has distant metastases. p53 gene mutation prevalence showed rs1042522 only has 15.7% mutation. There was 54.7% Deletion A and 45.3% Wild Type A detected in rs59758982, 87.5% Deletion A and 12.5% Wild Type A in rs35069695 and 92.2 % recorded for Deletion GAA in rs376546152. There was no significant result between these mutation with breast cancer molecular classification and breast cancer aggressiveness except for rs59758982 shows significant result with p value 0.04 (p <0.05). In regards on for p53 serum auto antibodies, 20.3% of the patients noted to be positive but it has no significant association with p53 gene mutations.. Conclusion: In this study, tissue p53 genetic mutation has no significant association with clinicopathological characteristic of breast cancer and the use of serum p53 auto antibody as biomarkers is inconclusive. 2016 Thesis http://eprints.usm.my/41862/ http://eprints.usm.my/41862/1/Dr._Fitreena_Anis-24_pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RC0321 Neuroscience Biological psychiatry Neuropsychiatry
spellingShingle	RC0321 Neuroscience Biological psychiatry Neuropsychiatry Amrank, Fitreena Anis The relationship of P53 gene mutation with clinicopathological characteristic in breast cancer
description	Background: P53 is a tumour suppresor gene. In breast cancer, p53 gene mutation were noted with frequency of about 30% (range 15 to 71%) and associated with poor prognosis. This study was perform determine p53 mutation association with clinicopathological characteristic in breast cancer and to assess the suitability of patients’ serum to detect p53 autoantibody. Methods: This study conducted in Hospital Seberang Jaya and Institut Perubatan dan Pergigian Termaju, Universiti Sains Malaysia. Six four breast cancer patients with available fresh breast cancer tissue that been kept under -80°C and with complete clinicopathological data involve in this study. These fresh breast tissues DNA extracted and 10sampels sent for DNA sequencing. The remaining 54samples proceeded with Polymerase Chains Reaction analysis based on the result from DNA sequencing. The serum of these patients was also taken for p53 autoantibody study using ELISA method. Results: The mean age of the patients in this study was 52.45±9.51 years. Most of the patients were Malay with 67.2% followed by Indian and Chinese with 17.2% and 15.6% respectively. About 51.6% of these patients undergone CT scan staging and 14.1% has distant metastases. p53 gene mutation prevalence showed rs1042522 only has 15.7% mutation. There was 54.7% Deletion A and 45.3% Wild Type A detected in rs59758982, 87.5% Deletion A and 12.5% Wild Type A in rs35069695 and 92.2 % recorded for Deletion GAA in rs376546152. There was no significant result between these mutation with breast cancer molecular classification and breast cancer aggressiveness except for rs59758982 shows significant result with p value 0.04 (p <0.05). In regards on for p53 serum auto antibodies, 20.3% of the patients noted to be positive but it has no significant association with p53 gene mutations.. Conclusion: In this study, tissue p53 genetic mutation has no significant association with clinicopathological characteristic of breast cancer and the use of serum p53 auto antibody as biomarkers is inconclusive.
format	Thesis
qualification_level	Master's degree
author	Amrank, Fitreena Anis
author_facet	Amrank, Fitreena Anis
author_sort	Amrank, Fitreena Anis
title	The relationship of P53 gene mutation with clinicopathological characteristic in breast cancer
title_short	The relationship of P53 gene mutation with clinicopathological characteristic in breast cancer
title_full	The relationship of P53 gene mutation with clinicopathological characteristic in breast cancer
title_fullStr	The relationship of P53 gene mutation with clinicopathological characteristic in breast cancer
title_full_unstemmed	The relationship of P53 gene mutation with clinicopathological characteristic in breast cancer
title_sort	relationship of p53 gene mutation with clinicopathological characteristic in breast cancer
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2016
url	http://eprints.usm.my/41862/1/Dr._Fitreena_Anis-24_pages.pdf
_version_	1747820985111805952

The relationship of P53 gene mutation with clinicopathological characteristic in breast cancer

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