Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei

Shigellosis or bacillary dysentery caused by Shigella spp. remains as significant health problem worldwide. Current detection and identification methods of the pathogens from stool specimens by culture method is relatively insensitive, generally time consuming (~ 2-5 days) and laborious. In this stu...

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Main Author:	Shakrin, Nik Noorul Shakira Mohamed
Format:	Thesis
Language:	English
Published:	2010
Subjects:	R735-854 Medical education Medical schools Research
Online Access:	http://eprints.usm.my/42167/1/NIK_NOORUL_SHAKIRA.pdf
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id	my-usm-ep.42167
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spelling	my-usm-ep.421672019-04-12T05:26:57Z Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei 2010-03 Shakrin, Nik Noorul Shakira Mohamed R735-854 Medical education. Medical schools. Research Shigellosis or bacillary dysentery caused by Shigella spp. remains as significant health problem worldwide. Current detection and identification methods of the pathogens from stool specimens by culture method is relatively insensitive, generally time consuming (~ 2-5 days) and laborious. In this study, thermostabilised Polymerase Chain Reaction (PCR) assays were developed for the detection of ompA genes in Shigella dysenteriae, Shigella flexneri and Shigella sonnei. The tests contained specific primers for the detection of S. dysenteriae, S. flexneri and S. sonnei respectively, freezed-dried or thermostabilised PCR reagents with Internal Control (IC) and gel loading dye. 2010-03 Thesis http://eprints.usm.my/42167/ http://eprints.usm.my/42167/1/NIK_NOORUL_SHAKIRA.pdf application/pdf en public masters Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R735-854 Medical education Medical schools Research
spellingShingle	R735-854 Medical education Medical schools Research Shakrin, Nik Noorul Shakira Mohamed Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei
description	Shigellosis or bacillary dysentery caused by Shigella spp. remains as significant health problem worldwide. Current detection and identification methods of the pathogens from stool specimens by culture method is relatively insensitive, generally time consuming (~ 2-5 days) and laborious. In this study, thermostabilised Polymerase Chain Reaction (PCR) assays were developed for the detection of ompA genes in Shigella dysenteriae, Shigella flexneri and Shigella sonnei. The tests contained specific primers for the detection of S. dysenteriae, S. flexneri and S. sonnei respectively, freezed-dried or thermostabilised PCR reagents with Internal Control (IC) and gel loading dye.
format	Thesis
qualification_level	Master's degree
author	Shakrin, Nik Noorul Shakira Mohamed
author_facet	Shakrin, Nik Noorul Shakira Mohamed
author_sort	Shakrin, Nik Noorul Shakira Mohamed
title	Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei
title_short	Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei
title_full	Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei
title_fullStr	Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei
title_full_unstemmed	Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei
title_sort	development of dna-based diagnostic tests for the detection of shigella dysenteriae, shigella flexneri and shigella sonnei
granting_institution	Universiti Sains Malaysia
granting_department	Institut Penyelidikan Perubatan Molekul
publishDate	2010
url	http://eprints.usm.my/42167/1/NIK_NOORUL_SHAKIRA.pdf
_version_	1747821032997126144

Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei

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