The Development Of Encapsulation-Dehydration And Vitrification Protocols For Protocorm-Like Bodies (PLBs) Of Dendrobium Sonia-28
The vitrification and encapsulation-dehydration methods of cryopreservation were applied on protocorm-like bodies (PLBs) of the orchid hybrid Dendrobium sonia-28, with survival assessments conducted through growth observations, visualand spectrophotometric 2,3,5-triphenyltetrazolium chloride (TTC) a...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | http://eprints.usm.my/42305/1/RANJETTA_POOBATHY.pdf |
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| Summary: | The vitrification and encapsulation-dehydration methods of cryopreservation were applied on protocorm-like bodies (PLBs) of the orchid hybrid Dendrobium sonia-28, with survival assessments conducted through growth observations, visualand spectrophotometric 2,3,5-triphenyltetrazolium chloride (TTC) assays. The best
survival (16.0% regeneration) for vitrification was obtained when 3-4mm PLBs were precultured in semi-solid half-strength MS medium supplemented with 0.4M
sucrose, loaded in a loading solution for 20 minutes, dehydrated for 50 minutes at 0°C in plant vitrification solution 2 (PVS2), cryopreserved in liquid nitrogen (LN)
for 24 hours, thawed in a 40±2°C water bath for 90 seconds, unloaded in 1.2M sucrose for 20 minutes, and regenerated in semi-solid half-strength MS medium
containing 2g.L-1 charcoal, with all media supplemented with 0.6mM ascorbic acid. |
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