Identification Of Biomarkers For Colorectal Cancer From Malaysian Patients

Colorectal cancer (CRC) is on the rise. However, biomarkers for indication of CRC progression is still lacking despite several advances in proteomic and microarray tools. This study aims to identify potential biomarkers for the diagnosis of CRC among Malaysian patients. In this study, sequential...

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Main Author:	Yeoh, Lay Chin
Format:	Thesis
Language:	English
Published:	2010
Subjects:	RS1-441 Pharmacy and materia medica
Online Access:	http://eprints.usm.my/42493/1/YEOH_LAY_CHIN_HJ.pdf
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id	my-usm-ep.42493
record_format	uketd_dc
spelling	my-usm-ep.424932019-04-12T05:26:52Z Identification Of Biomarkers For Colorectal Cancer From Malaysian Patients 2010-06 Yeoh, Lay Chin RS1-441 Pharmacy and materia medica Colorectal cancer (CRC) is on the rise. However, biomarkers for indication of CRC progression is still lacking despite several advances in proteomic and microarray tools. This study aims to identify potential biomarkers for the diagnosis of CRC among Malaysian patients. In this study, sequential extraction was first performed using Tris buffer followed by TLB buffer in order to reduce the complexity of the CRC proteome. The tissues used were surgical resections of normal mucosa and cancerous tissues from 26 CRC patients. The extracted proteins were separated by 2D gel electrophoresis. The differentially expressed protein spots were then mapped for further protein identification by the use of liquid chromatography tandem mass spectrometry (LC-MS/MS). We have identified 37 hydrophilic proteins in the Tris extracts and 24 hydrophobic proteins in the thiourea lysis buffer (TLB) extracts from the samples collected. Leukocyte elastase inhibitor (LEI) and 60 kDa heat shock protein (HSP60) were the potential biomarkers for CRC analyzed from the Tris extracts whereas triosephosphate isomerase (TPI) and annexin A3 were the potential biomarkers for CRC analyzed from the TLB extracts. Moreover, LEI was the potential biomarker for CRC patients at stage III in combination with either moderately differentiated adenocarcinoma (MDA), well differentiated adenocarcinoma (WDA) or lymph node metastasis CRC. 2010-06 Thesis http://eprints.usm.my/42493/ http://eprints.usm.my/42493/1/YEOH_LAY_CHIN_HJ.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Farmasi
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RS1-441 Pharmacy and materia medica
spellingShingle	RS1-441 Pharmacy and materia medica Yeoh, Lay Chin Identification Of Biomarkers For Colorectal Cancer From Malaysian Patients
description	Colorectal cancer (CRC) is on the rise. However, biomarkers for indication of CRC progression is still lacking despite several advances in proteomic and microarray tools. This study aims to identify potential biomarkers for the diagnosis of CRC among Malaysian patients. In this study, sequential extraction was first performed using Tris buffer followed by TLB buffer in order to reduce the complexity of the CRC proteome. The tissues used were surgical resections of normal mucosa and cancerous tissues from 26 CRC patients. The extracted proteins were separated by 2D gel electrophoresis. The differentially expressed protein spots were then mapped for further protein identification by the use of liquid chromatography tandem mass spectrometry (LC-MS/MS). We have identified 37 hydrophilic proteins in the Tris extracts and 24 hydrophobic proteins in the thiourea lysis buffer (TLB) extracts from the samples collected. Leukocyte elastase inhibitor (LEI) and 60 kDa heat shock protein (HSP60) were the potential biomarkers for CRC analyzed from the Tris extracts whereas triosephosphate isomerase (TPI) and annexin A3 were the potential biomarkers for CRC analyzed from the TLB extracts. Moreover, LEI was the potential biomarker for CRC patients at stage III in combination with either moderately differentiated adenocarcinoma (MDA), well differentiated adenocarcinoma (WDA) or lymph node metastasis CRC.
format	Thesis
qualification_level	Master's degree
author	Yeoh, Lay Chin
author_facet	Yeoh, Lay Chin
author_sort	Yeoh, Lay Chin
title	Identification Of Biomarkers For Colorectal Cancer From Malaysian Patients
title_short	Identification Of Biomarkers For Colorectal Cancer From Malaysian Patients
title_full	Identification Of Biomarkers For Colorectal Cancer From Malaysian Patients
title_fullStr	Identification Of Biomarkers For Colorectal Cancer From Malaysian Patients
title_full_unstemmed	Identification Of Biomarkers For Colorectal Cancer From Malaysian Patients
title_sort	identification of biomarkers for colorectal cancer from malaysian patients
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Farmasi
publishDate	2010
url	http://eprints.usm.my/42493/1/YEOH_LAY_CHIN_HJ.pdf
_version_	1747821076319043584

Identification Of Biomarkers For Colorectal Cancer From Malaysian Patients

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