Development of recombinant Mycobacterium smegmatis (MS) expressing B- and T-cell epitopes of latency associated antigens of Mycobacterium tuberculosis (MTB) as a TB vaccine candidate

Tuberculosis (TB) remains a major public health problem worldwide. Nearly one-third of the world population is latently infected with Mycobacterium tuberculosis (MTB) and 5 - 10 % of infected individuals will develop active disease during their life time. Bacille Calmette–Guérin (BCG) efficiently pr...

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Main Author:	Ismail, Mohd Zakuan
Format:	Thesis
Language:	English
Published:	2017
Subjects:	RC705-779 Diseases of the respiratory system
Online Access:	http://eprints.usm.my/43103/1/Dr._Mohd_Zakuan_Ismail-24_pages.pdf
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id	my-usm-ep.43103
record_format	uketd_dc
spelling	my-usm-ep.431032019-04-12T05:25:00Z Development of recombinant Mycobacterium smegmatis (MS) expressing B- and T-cell epitopes of latency associated antigens of Mycobacterium tuberculosis (MTB) as a TB vaccine candidate 2017-06 Ismail, Mohd Zakuan RC705-779 Diseases of the respiratory system Tuberculosis (TB) remains a major public health problem worldwide. Nearly one-third of the world population is latently infected with Mycobacterium tuberculosis (MTB) and 5 - 10 % of infected individuals will develop active disease during their life time. Bacille Calmette–Guérin (BCG) efficiently protects against severe disease manifestations in children, but does not prevent the establishment of latent TB or reactivation of TB pulmonary disease in adult life. Therefore, in order to control this scourge from exacerbating, the development of an effective TB vaccine is an urgent priority. The expression of MTB antigens in Mycobacterium smegmatis (MS) is one of the potential strategies for the development of new generation vaccines against TB. This study focused on the construction and evaluation of immunogenicity in Balb/C mice of a recombinant MS (rMS) expressing B- and T-cell epitopes of Latency-Associated Antigens (LAA) (Rv2005c, Rv2031c, Rv3130c, Rv3127) of MTB (rMS081). The capacity of rMS081 to induce specific humoral and cellular immune responses against expressed B- and T-cell epitopes was evaluated. Total specific Immunoglobulin G (IgG) showed a significant increase against all epitopes (Rv2005c, Rv2031c, Rv3130c, Rv3127) in the sera of rMS081-immunized mice compared with the control group. In the experimental conditions evaluated, there were no significant increase in the cytokines, Interferon-gamma (IFN-), Interleukin (IL)-2 and IL-4 against the LAA epitopes in the rMS081-immunized mice compared with the control group. The results obtained support the future evaluation of rMS081 as a vaccine candidate against TB in challenge studies. 2017-06 Thesis http://eprints.usm.my/43103/ http://eprints.usm.my/43103/1/Dr._Mohd_Zakuan_Ismail-24_pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RC705-779 Diseases of the respiratory system
spellingShingle	RC705-779 Diseases of the respiratory system Ismail, Mohd Zakuan Development of recombinant Mycobacterium smegmatis (MS) expressing B- and T-cell epitopes of latency associated antigens of Mycobacterium tuberculosis (MTB) as a TB vaccine candidate
description	Tuberculosis (TB) remains a major public health problem worldwide. Nearly one-third of the world population is latently infected with Mycobacterium tuberculosis (MTB) and 5 - 10 % of infected individuals will develop active disease during their life time. Bacille Calmette–Guérin (BCG) efficiently protects against severe disease manifestations in children, but does not prevent the establishment of latent TB or reactivation of TB pulmonary disease in adult life. Therefore, in order to control this scourge from exacerbating, the development of an effective TB vaccine is an urgent priority. The expression of MTB antigens in Mycobacterium smegmatis (MS) is one of the potential strategies for the development of new generation vaccines against TB. This study focused on the construction and evaluation of immunogenicity in Balb/C mice of a recombinant MS (rMS) expressing B- and T-cell epitopes of Latency-Associated Antigens (LAA) (Rv2005c, Rv2031c, Rv3130c, Rv3127) of MTB (rMS081). The capacity of rMS081 to induce specific humoral and cellular immune responses against expressed B- and T-cell epitopes was evaluated. Total specific Immunoglobulin G (IgG) showed a significant increase against all epitopes (Rv2005c, Rv2031c, Rv3130c, Rv3127) in the sera of rMS081-immunized mice compared with the control group. In the experimental conditions evaluated, there were no significant increase in the cytokines, Interferon-gamma (IFN-), Interleukin (IL)-2 and IL-4 against the LAA epitopes in the rMS081-immunized mice compared with the control group. The results obtained support the future evaluation of rMS081 as a vaccine candidate against TB in challenge studies.
format	Thesis
qualification_level	Master's degree
author	Ismail, Mohd Zakuan
author_facet	Ismail, Mohd Zakuan
author_sort	Ismail, Mohd Zakuan
title	Development of recombinant Mycobacterium smegmatis (MS) expressing B- and T-cell epitopes of latency associated antigens of Mycobacterium tuberculosis (MTB) as a TB vaccine candidate
title_short	Development of recombinant Mycobacterium smegmatis (MS) expressing B- and T-cell epitopes of latency associated antigens of Mycobacterium tuberculosis (MTB) as a TB vaccine candidate
title_full	Development of recombinant Mycobacterium smegmatis (MS) expressing B- and T-cell epitopes of latency associated antigens of Mycobacterium tuberculosis (MTB) as a TB vaccine candidate
title_fullStr	Development of recombinant Mycobacterium smegmatis (MS) expressing B- and T-cell epitopes of latency associated antigens of Mycobacterium tuberculosis (MTB) as a TB vaccine candidate
title_full_unstemmed	Development of recombinant Mycobacterium smegmatis (MS) expressing B- and T-cell epitopes of latency associated antigens of Mycobacterium tuberculosis (MTB) as a TB vaccine candidate
title_sort	development of recombinant mycobacterium smegmatis (ms) expressing b- and t-cell epitopes of latency associated antigens of mycobacterium tuberculosis (mtb) as a tb vaccine candidate
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2017
url	http://eprints.usm.my/43103/1/Dr._Mohd_Zakuan_Ismail-24_pages.pdf
_version_	1747821164766429184

Development of recombinant Mycobacterium smegmatis (MS) expressing B- and T-cell epitopes of latency associated antigens of Mycobacterium tuberculosis (MTB) as a TB vaccine candidate

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