Functional Characterization Of The Outer Membrane Protein TolC of Salmonella enterica subspecies enterica serovar Typhi And Its Association With Virulence

Although Salmonella enterica subspecies enterica serovar Typhi (S. Typhi) has been well studied, only a few of its outer membrane proteins (OMPs) have been functionally characterized. In this study, a tolC deletion mutant was generated to study the biological functions of TolC in S. Typhi strain tha...

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Main Author:	Hussain, Ashraf
Format:	Thesis
Language:	English
Published:	2018
Subjects:	R735-854 Medical education Medical schools Research
Online Access:	http://eprints.usm.my/43611/1/ASHRAF%20HUSSAIN.pdf
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id	my-usm-ep.43611
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spelling	my-usm-ep.436112019-04-12T05:24:52Z Functional Characterization Of The Outer Membrane Protein TolC of Salmonella enterica subspecies enterica serovar Typhi And Its Association With Virulence 2018-02 Hussain, Ashraf R735-854 Medical education. Medical schools. Research Although Salmonella enterica subspecies enterica serovar Typhi (S. Typhi) has been well studied, only a few of its outer membrane proteins (OMPs) have been functionally characterized. In this study, a tolC deletion mutant was generated to study the biological functions of TolC in S. Typhi strain that was isolated from an acute typhoid patient. Deletion of TolC caused increase susceptibility to a range of antimicrobials challenge, compromised membrane integrity, and reduced efflux activity of S. Typhi. In addition, the ΔtolC mutant was shown to have lower adhesion and invasion capability in an in vitro human cell culture infection model. 2018-02 Thesis http://eprints.usm.my/43611/ http://eprints.usm.my/43611/1/ASHRAF%20HUSSAIN.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R735-854 Medical education Medical schools Research
spellingShingle	R735-854 Medical education Medical schools Research Hussain, Ashraf Functional Characterization Of The Outer Membrane Protein TolC of Salmonella enterica subspecies enterica serovar Typhi And Its Association With Virulence
description	Although Salmonella enterica subspecies enterica serovar Typhi (S. Typhi) has been well studied, only a few of its outer membrane proteins (OMPs) have been functionally characterized. In this study, a tolC deletion mutant was generated to study the biological functions of TolC in S. Typhi strain that was isolated from an acute typhoid patient. Deletion of TolC caused increase susceptibility to a range of antimicrobials challenge, compromised membrane integrity, and reduced efflux activity of S. Typhi. In addition, the ΔtolC mutant was shown to have lower adhesion and invasion capability in an in vitro human cell culture infection model.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Hussain, Ashraf
author_facet	Hussain, Ashraf
author_sort	Hussain, Ashraf
title	Functional Characterization Of The Outer Membrane Protein TolC of Salmonella enterica subspecies enterica serovar Typhi And Its Association With Virulence
title_short	Functional Characterization Of The Outer Membrane Protein TolC of Salmonella enterica subspecies enterica serovar Typhi And Its Association With Virulence
title_full	Functional Characterization Of The Outer Membrane Protein TolC of Salmonella enterica subspecies enterica serovar Typhi And Its Association With Virulence
title_fullStr	Functional Characterization Of The Outer Membrane Protein TolC of Salmonella enterica subspecies enterica serovar Typhi And Its Association With Virulence
title_full_unstemmed	Functional Characterization Of The Outer Membrane Protein TolC of Salmonella enterica subspecies enterica serovar Typhi And Its Association With Virulence
title_sort	functional characterization of the outer membrane protein tolc of salmonella enterica subspecies enterica serovar typhi and its association with virulence
granting_institution	Universiti Sains Malaysia
granting_department	Institut Penyelidikan Perubatan Molekul
publishDate	2018
url	http://eprints.usm.my/43611/1/ASHRAF%20HUSSAIN.pdf
_version_	1747821247661604864

Functional Characterization Of The Outer Membrane Protein TolC of Salmonella enterica subspecies enterica serovar Typhi And Its Association With Virulence

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