Optimization of sugar assimilation test for identification of clinically isolated yeast

The study to developed the sugar assimilation test for identification of clinically isolated yeast was done using the swab lawning method and the method of incorporation of suspension on to medium. The result obtained, showed that the swab lawning method was not feasible to be used for identifyin...

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Main Author:	Othman, Afaf Alani Che
Format:	Thesis
Language:	English
Published:	2004
Subjects:	R Medicine
Online Access:	http://eprints.usm.my/44144/1/LI...Afaf%20Alani%20Binti%20Che%20Othman...2004...-24%20pages.pdf
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id	my-usm-ep.44144
record_format	uketd_dc
spelling	my-usm-ep.441442020-10-22T03:03:26Z Optimization of sugar assimilation test for identification of clinically isolated yeast 2004 Othman, Afaf Alani Che R Medicine The study to developed the sugar assimilation test for identification of clinically isolated yeast was done using the swab lawning method and the method of incorporation of suspension on to medium. The result obtained, showed that the swab lawning method was not feasible to be used for identifying clinically isolated yeast based on the sugar assimilation test However, the method of incorporation of suspension on medium was comparatively better and was carried out by optimizing the test by varying the sugar concentration, medium pH and incubation temperature. Candida spp. were screened using 14 types of sugars. The optimized conditions for identification purpose was 20% ( w/v) of sugar concentration, medium pH of 5.6 and the incubation temperature of 30°C for both Candida albican and Candida tropicalis. Under these optimized conditions for identification, Candida a/bican did not utilized cellobiose, whereas Candida tropica/is utilized cellobiose. But the optimum growth conditions for both species were same. Therefore the method of incorporation of suspension under the optimized conditions may be used in the identification of clinically isolated yeast especially in differentiating Candida a/bican and Candida tropical is. 2004 Thesis http://eprints.usm.my/44144/ http://eprints.usm.my/44144/1/LI...Afaf%20Alani%20Binti%20Che%20Othman...2004...-24%20pages.pdf application/pdf en public other Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine
spellingShingle	R Medicine Othman, Afaf Alani Che Optimization of sugar assimilation test for identification of clinically isolated yeast
description	The study to developed the sugar assimilation test for identification of clinically isolated yeast was done using the swab lawning method and the method of incorporation of suspension on to medium. The result obtained, showed that the swab lawning method was not feasible to be used for identifying clinically isolated yeast based on the sugar assimilation test However, the method of incorporation of suspension on medium was comparatively better and was carried out by optimizing the test by varying the sugar concentration, medium pH and incubation temperature. Candida spp. were screened using 14 types of sugars. The optimized conditions for identification purpose was 20% ( w/v) of sugar concentration, medium pH of 5.6 and the incubation temperature of 30°C for both Candida albican and Candida tropicalis. Under these optimized conditions for identification, Candida a/bican did not utilized cellobiose, whereas Candida tropica/is utilized cellobiose. But the optimum growth conditions for both species were same. Therefore the method of incorporation of suspension under the optimized conditions may be used in the identification of clinically isolated yeast especially in differentiating Candida a/bican and Candida tropical is.
format	Thesis
qualification_level	other
author	Othman, Afaf Alani Che
author_facet	Othman, Afaf Alani Che
author_sort	Othman, Afaf Alani Che
title	Optimization of sugar assimilation test for identification of clinically isolated yeast
title_short	Optimization of sugar assimilation test for identification of clinically isolated yeast
title_full	Optimization of sugar assimilation test for identification of clinically isolated yeast
title_fullStr	Optimization of sugar assimilation test for identification of clinically isolated yeast
title_full_unstemmed	Optimization of sugar assimilation test for identification of clinically isolated yeast
title_sort	optimization of sugar assimilation test for identification of clinically isolated yeast
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kesihatan
publishDate	2004
url	http://eprints.usm.my/44144/1/LI...Afaf%20Alani%20Binti%20Che%20Othman...2004...-24%20pages.pdf
_version_	1747821334354722816

Optimization of sugar assimilation test for identification of clinically isolated yeast

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