Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28
Effective propagation of protocorm-like bodies (PLBs) of Dendrobium sonia-28 was achieved by using micropropagation technique. The highest PLBs proliferation within 21 days was observed in semi-solid media over liquid culture. Half strength of Murashige and Skoog (MS) media produced the highest PLBs...
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2012
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my-usm-ep.447762019-07-01T02:11:17Z Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28 2012-06 Julkifle, Advina Lizah QH1 Natural history (General - Including nature conservation, geographical distribution) Effective propagation of protocorm-like bodies (PLBs) of Dendrobium sonia-28 was achieved by using micropropagation technique. The highest PLBs proliferation within 21 days was observed in semi-solid media over liquid culture. Half strength of Murashige and Skoog (MS) media produced the highest PLBs growth rate percentage compared to full and double strength MS media in semi-solid and liquid cultures. In sucrose treatment, the highest PLBs growth rate percentage was in 10 g.L-1 sucrose. In the conditions of absence of sucrose (0 g.L-1) or excessive of sucrose (30 g.L-1) had proven detrimental for the PLBs growth and development. 2012-06 Thesis http://eprints.usm.my/44776/ http://eprints.usm.my/44776/1/ADVINA%20LIZAH%20BINTI%20JULKIFLE.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat |
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Universiti Sains Malaysia |
collection |
USM Institutional Repository |
language |
English |
topic |
QH1 Natural history (General - Including nature conservation geographical distribution) |
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QH1 Natural history (General - Including nature conservation geographical distribution) Julkifle, Advina Lizah Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28 |
description |
Effective propagation of protocorm-like bodies (PLBs) of Dendrobium sonia-28 was achieved by using micropropagation technique. The highest PLBs proliferation within 21 days was observed in semi-solid media over liquid culture. Half strength of Murashige and Skoog (MS) media produced the highest PLBs growth rate percentage compared to full and double strength MS media in semi-solid and liquid cultures. In sucrose treatment, the highest PLBs growth rate percentage was in 10 g.L-1 sucrose. In the conditions of absence of sucrose (0 g.L-1) or excessive of sucrose (30 g.L-1) had proven detrimental for the PLBs growth and development. |
format |
Thesis |
qualification_level |
Master's degree |
author |
Julkifle, Advina Lizah |
author_facet |
Julkifle, Advina Lizah |
author_sort |
Julkifle, Advina Lizah |
title |
Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28 |
title_short |
Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28 |
title_full |
Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28 |
title_fullStr |
Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28 |
title_full_unstemmed |
Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28 |
title_sort |
agrobacterium-mediated transformation of dendrobium sonia-28 |
granting_institution |
Universiti Sains Malaysia |
granting_department |
Pusat Pengajian Sains Kajihayat |
publishDate |
2012 |
url |
http://eprints.usm.my/44776/1/ADVINA%20LIZAH%20BINTI%20JULKIFLE.pdf |
_version_ |
1747821396109557760 |