Decolourisation And Cod Reduction Of Methylene Blue And Dye Wastewater Using Sphingomonas Paucimobilis

Microbial decolourisation and degradation is an environmentally friendly and cost competitive alternative process in the treatment of dye wastewater. In this study, the decolourisation and degradation of Methylene blue (MB) by a Sphingomonas paucimobilis strain isolated from industrial wastewater wa...

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Main Author:	Hasnam, Che Noraini Che
Format:	Thesis
Language:	English
Published:	2013
Subjects:	T11.95-12.5 Industrial directories
Online Access:	http://eprints.usm.my/45065/1/Che%20Noraini%20Binti%20Che%20Hasnam24.pdf
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spelling	my-usm-ep.450652019-07-25T08:29:43Z Decolourisation And Cod Reduction Of Methylene Blue And Dye Wastewater Using Sphingomonas Paucimobilis 2013-07 Hasnam, Che Noraini Che T11.95-12.5 Industrial directories Microbial decolourisation and degradation is an environmentally friendly and cost competitive alternative process in the treatment of dye wastewater. In this study, the decolourisation and degradation of Methylene blue (MB) by a Sphingomonas paucimobilis strain isolated from industrial wastewater was investigated. Experimental results were assessed in terms of chemical oxygen demand (COD) reduction and decolourisation of dye wastewater using S. paucimobilis. It was found to be capable in reducing the concentration of COD at 92.99 % and achieving 85 % in decolourisation at optimal conditions of pH 9.0 and at inoculums size of 185-mL within incubation HRT of 5 days. Analysis of samples extracted from decolourised culture flasks at pH 9.0 using Fourier transform infrared (FTIR) spectroscopy confirmed that the mechanism of colour removal was due to biodegradation. In addition, scanning and transmission electron microscopy revealed the secretion of exopolysaccharides (EPS) by S. paucimobilis cells on exposure to dye that showed a probable physiological defence mechanism to ensure controlled diffusion of dye molecules into cellular structures. The experiment were performed under steady state conditions to determine the kinetic coefficients such as growth yield (Y), specific biomass decay (Kd), maximum specific substrate utilisation (K), saturation constant for substrate (Ks), maximum specific biomass growth rate (μmax), and critical retention time (θc). The kinetic coefficients were evaluated using Monod equation. The bio-kinetic coefficients Y, Kd, k, Ks, and μmaxwere found to be 1.879, 0.013 per day, 0.540 per day, 4214.7 mg/L and 1.015 per day, respectively. 2013-07 Thesis http://eprints.usm.my/45065/ http://eprints.usm.my/45065/1/Che%20Noraini%20Binti%20Che%20Hasnam24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Teknologi Industri
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	T11.95-12.5 Industrial directories
spellingShingle	T11.95-12.5 Industrial directories Hasnam, Che Noraini Che Decolourisation And Cod Reduction Of Methylene Blue And Dye Wastewater Using Sphingomonas Paucimobilis
description	Microbial decolourisation and degradation is an environmentally friendly and cost competitive alternative process in the treatment of dye wastewater. In this study, the decolourisation and degradation of Methylene blue (MB) by a Sphingomonas paucimobilis strain isolated from industrial wastewater was investigated. Experimental results were assessed in terms of chemical oxygen demand (COD) reduction and decolourisation of dye wastewater using S. paucimobilis. It was found to be capable in reducing the concentration of COD at 92.99 % and achieving 85 % in decolourisation at optimal conditions of pH 9.0 and at inoculums size of 185-mL within incubation HRT of 5 days. Analysis of samples extracted from decolourised culture flasks at pH 9.0 using Fourier transform infrared (FTIR) spectroscopy confirmed that the mechanism of colour removal was due to biodegradation. In addition, scanning and transmission electron microscopy revealed the secretion of exopolysaccharides (EPS) by S. paucimobilis cells on exposure to dye that showed a probable physiological defence mechanism to ensure controlled diffusion of dye molecules into cellular structures. The experiment were performed under steady state conditions to determine the kinetic coefficients such as growth yield (Y), specific biomass decay (Kd), maximum specific substrate utilisation (K), saturation constant for substrate (Ks), maximum specific biomass growth rate (μmax), and critical retention time (θc). The kinetic coefficients were evaluated using Monod equation. The bio-kinetic coefficients Y, Kd, k, Ks, and μmaxwere found to be 1.879, 0.013 per day, 0.540 per day, 4214.7 mg/L and 1.015 per day, respectively.
format	Thesis
qualification_level	Master's degree
author	Hasnam, Che Noraini Che
author_facet	Hasnam, Che Noraini Che
author_sort	Hasnam, Che Noraini Che
title	Decolourisation And Cod Reduction Of Methylene Blue And Dye Wastewater Using Sphingomonas Paucimobilis
title_short	Decolourisation And Cod Reduction Of Methylene Blue And Dye Wastewater Using Sphingomonas Paucimobilis
title_full	Decolourisation And Cod Reduction Of Methylene Blue And Dye Wastewater Using Sphingomonas Paucimobilis
title_fullStr	Decolourisation And Cod Reduction Of Methylene Blue And Dye Wastewater Using Sphingomonas Paucimobilis
title_full_unstemmed	Decolourisation And Cod Reduction Of Methylene Blue And Dye Wastewater Using Sphingomonas Paucimobilis
title_sort	decolourisation and cod reduction of methylene blue and dye wastewater using sphingomonas paucimobilis
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Teknologi Industri
publishDate	2013
url	http://eprints.usm.my/45065/1/Che%20Noraini%20Binti%20Che%20Hasnam24.pdf
_version_	1747821448738635776

Decolourisation And Cod Reduction Of Methylene Blue And Dye Wastewater Using Sphingomonas Paucimobilis

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