Optimization Of Polyhydroxyalkanoate (PHA) Production By Burkholderia Cepacia BPT1213 Using Waste Glycerol As The Sole Carbon Source

Polyhydroxyalkanoate (PHA) is touted as an environmental friendly alternative to recalcitrant petrochemical derived plastics. Due to high production cost issue, waste glycerol produced from biodiesel industry has potential as cheaper carbon source for PHA synthesis by microorganisms. The objectives...

Full description

Saved in:
Bibliographic Details
Main Author: Mohd Zain, Nor Fauziah
Format: Thesis
Language:English
Published: 2017
Subjects:
Online Access:http://eprints.usm.my/45450/1/NOR%20FAUZIAH%20MOHD%20ZAIN.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Polyhydroxyalkanoate (PHA) is touted as an environmental friendly alternative to recalcitrant petrochemical derived plastics. Due to high production cost issue, waste glycerol produced from biodiesel industry has potential as cheaper carbon source for PHA synthesis by microorganisms. The objectives of this study were to optimize and scale up the production of PHA by a local isolate, Burkholderia cepacia BPT1213, from the shake flask system to a 2.5 L bioreactor system. Throughout this study, the strain was grown in minimal salt medium (MSM) supplemented with 2% (v/v) of waste glycerol (86.70% purity), a by-product of the production biodiesel from palm oil as the sole carbon source.