The Role Of Endogenous Proteolytic Enzymes In The Production Of Budu

The objective of this study was to determine whether the transformation of fish protein during budu fermentation was caused by endogenous enzymes or exogenous enzymes produced by microorganism present in the viscera and the muscles of the fish. Malaysian fish sauce, budu was produced by incubating a...

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Main Author:	Ng, Yen Fen
Format:	Thesis
Language:	English
Published:	2011
Subjects:	T11.95-12.5 Industrial directories
Online Access:	http://eprints.usm.my/45669/1/NG%20YEE%20FEN_HJ.pdf
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id	my-usm-ep.45669
record_format	uketd_dc
spelling	my-usm-ep.456692019-10-16T01:23:53Z The Role Of Endogenous Proteolytic Enzymes In The Production Of Budu 2011-06 Ng, Yen Fen T11.95-12.5 Industrial directories The objective of this study was to determine whether the transformation of fish protein during budu fermentation was caused by endogenous enzymes or exogenous enzymes produced by microorganism present in the viscera and the muscles of the fish. Malaysian fish sauce, budu was produced by incubating a mixture of anchovies (Encrasicholina punctifer) and salt in a ratio of 3:1. Four types of treatment were carried out on the fish: i.e. whole, un-irradiated fish as the control (budu C); whole, 10 kGy gamma irradiated fish (budu R); eviscerated, un-irradiated fish (budu E), and eviscerated, 10 kGy gamma irradiated fish (budu A). Proteolytic activity was significantly higher in budu prepared from whole, un-eviscerated fish (C) compared to budu prepared from whole but irradiated fish (R). There was no difference (P>0.05) in proteolytic activity in budu prepared from both eviscerated, irradiated (A) and eviscerated and un-irradiated fish (E). Inhibition studies showed that the predominant proteases in budu prepared from whole and irradiated (R) fish and whole and un-irradiated (C) is trypsin-like protease belonging to the serine protease family. Low inhibition by pepstatin A and EDTA on protease in budu indicates the lack of involvement of aspartic protease and metalloprotease in the budu production. Low proteolytic bacterial counts in all budu suggested that proteolytic bacteria plays; only a minor role in the protein hydrolysis of fish. Using the API system, Bacillus, Staphylococcus and, Micrococcus were identified as the proteolytic bacteria in all budu samples. 2011-06 Thesis http://eprints.usm.my/45669/ http://eprints.usm.my/45669/1/NG%20YEE%20FEN_HJ.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Teknologi Industri
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	T11.95-12.5 Industrial directories
spellingShingle	T11.95-12.5 Industrial directories Ng, Yen Fen The Role Of Endogenous Proteolytic Enzymes In The Production Of Budu
description	The objective of this study was to determine whether the transformation of fish protein during budu fermentation was caused by endogenous enzymes or exogenous enzymes produced by microorganism present in the viscera and the muscles of the fish. Malaysian fish sauce, budu was produced by incubating a mixture of anchovies (Encrasicholina punctifer) and salt in a ratio of 3:1. Four types of treatment were carried out on the fish: i.e. whole, un-irradiated fish as the control (budu C); whole, 10 kGy gamma irradiated fish (budu R); eviscerated, un-irradiated fish (budu E), and eviscerated, 10 kGy gamma irradiated fish (budu A). Proteolytic activity was significantly higher in budu prepared from whole, un-eviscerated fish (C) compared to budu prepared from whole but irradiated fish (R). There was no difference (P>0.05) in proteolytic activity in budu prepared from both eviscerated, irradiated (A) and eviscerated and un-irradiated fish (E). Inhibition studies showed that the predominant proteases in budu prepared from whole and irradiated (R) fish and whole and un-irradiated (C) is trypsin-like protease belonging to the serine protease family. Low inhibition by pepstatin A and EDTA on protease in budu indicates the lack of involvement of aspartic protease and metalloprotease in the budu production. Low proteolytic bacterial counts in all budu suggested that proteolytic bacteria plays; only a minor role in the protein hydrolysis of fish. Using the API system, Bacillus, Staphylococcus and, Micrococcus were identified as the proteolytic bacteria in all budu samples.
format	Thesis
qualification_level	Master's degree
author	Ng, Yen Fen
author_facet	Ng, Yen Fen
author_sort	Ng, Yen Fen
title	The Role Of Endogenous Proteolytic Enzymes In The Production Of Budu
title_short	The Role Of Endogenous Proteolytic Enzymes In The Production Of Budu
title_full	The Role Of Endogenous Proteolytic Enzymes In The Production Of Budu
title_fullStr	The Role Of Endogenous Proteolytic Enzymes In The Production Of Budu
title_full_unstemmed	The Role Of Endogenous Proteolytic Enzymes In The Production Of Budu
title_sort	role of endogenous proteolytic enzymes in the production of budu
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Teknologi Industri
publishDate	2011
url	http://eprints.usm.my/45669/1/NG%20YEE%20FEN_HJ.pdf
_version_	1747821543595966464

The Role Of Endogenous Proteolytic Enzymes In The Production Of Budu

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