The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters

Background and objective: In recent years, “double hit” and “double protein” involving gene rearrangement and protein expression of MYC and BCL2 and/or BCL6 are the most used terms to describe poor prognostic factors in diffuse large B-cell lymphoma (DLBCL) This current study is to determine the...

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Main Author: Wahab, Wan Nor Najmiyah Wan Abdul
Format: Thesis
Language:English
Published: 2017
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Online Access:http://eprints.usm.my/45682/1/Dr.%20Wan%20Nor%20Najmiyah%20Wan%20Abdul%20Wahab-24%20pages.pdf
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spelling my-usm-ep.456822019-10-17T08:35:53Z The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters 2017 Wahab, Wan Nor Najmiyah Wan Abdul RC Internal medicine Background and objective: In recent years, “double hit” and “double protein” involving gene rearrangement and protein expression of MYC and BCL2 and/or BCL6 are the most used terms to describe poor prognostic factors in diffuse large B-cell lymphoma (DLBCL) This current study is to determine the frequency of double or triple protein expression by using immunohistochemistry (IHC) and relating the result with clinicopathological features and cell of origin (COO) classification. Material and methods: We conducted a cross sectional study by using 29 archived formalin-fixed paraffin embedded tissue blocks of DLBCL. All the samples were evaluated for expression of MYC, BCL2 and BCL6 by IHC. In addition, the subgrouping of COO DLBCL was determined by expression of CD10, BCL6 and MUM1 based on Hans classification. Result: Among the 29 cases, MYC, BCL2 and BCL6 proteins were detected in 72.4%, 62.1% and 62.1% of patients, respectively. Concurrent expression (MYC positive/BCL2 positive and/or BCL6 positive) was present in 58.6% of patients. 34.5% were categorized as germinal centre like (GCB) subgroup and 65.5% were categorized as non germinal centre like (non-GCB) subgroup. Among the clinicopathological features, the double/triple protein expression lymphoma was significantly associated with elevated LDH level (p=0.018), IPI score (p=0.003), Ann Arbor stage (p=0.011) and complete response rate (p=0.011Conclusion: Double/triple protein lymphoma was strongly associated more adverse clinical risk factors. Thus, analyses of MYC, BCL2 and BCL6 expression by IHC represents a rapid and inexpensive approach to risk-stratify patients with DLBCL at diagnosis. 2017 Thesis http://eprints.usm.my/45682/ http://eprints.usm.my/45682/1/Dr.%20Wan%20Nor%20Najmiyah%20Wan%20Abdul%20Wahab-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic RC Internal medicine
spellingShingle RC Internal medicine
Wahab, Wan Nor Najmiyah Wan Abdul
The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters
description Background and objective: In recent years, “double hit” and “double protein” involving gene rearrangement and protein expression of MYC and BCL2 and/or BCL6 are the most used terms to describe poor prognostic factors in diffuse large B-cell lymphoma (DLBCL) This current study is to determine the frequency of double or triple protein expression by using immunohistochemistry (IHC) and relating the result with clinicopathological features and cell of origin (COO) classification. Material and methods: We conducted a cross sectional study by using 29 archived formalin-fixed paraffin embedded tissue blocks of DLBCL. All the samples were evaluated for expression of MYC, BCL2 and BCL6 by IHC. In addition, the subgrouping of COO DLBCL was determined by expression of CD10, BCL6 and MUM1 based on Hans classification. Result: Among the 29 cases, MYC, BCL2 and BCL6 proteins were detected in 72.4%, 62.1% and 62.1% of patients, respectively. Concurrent expression (MYC positive/BCL2 positive and/or BCL6 positive) was present in 58.6% of patients. 34.5% were categorized as germinal centre like (GCB) subgroup and 65.5% were categorized as non germinal centre like (non-GCB) subgroup. Among the clinicopathological features, the double/triple protein expression lymphoma was significantly associated with elevated LDH level (p=0.018), IPI score (p=0.003), Ann Arbor stage (p=0.011) and complete response rate (p=0.011Conclusion: Double/triple protein lymphoma was strongly associated more adverse clinical risk factors. Thus, analyses of MYC, BCL2 and BCL6 expression by IHC represents a rapid and inexpensive approach to risk-stratify patients with DLBCL at diagnosis.
format Thesis
qualification_level Master's degree
author Wahab, Wan Nor Najmiyah Wan Abdul
author_facet Wahab, Wan Nor Najmiyah Wan Abdul
author_sort Wahab, Wan Nor Najmiyah Wan Abdul
title The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters
title_short The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters
title_full The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters
title_fullStr The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters
title_full_unstemmed The immunohistochemical studies of double or triple protein lymphoma in diffuse large B-cell lymphoma and their correlation with clinicopathological parameters
title_sort immunohistochemical studies of double or triple protein lymphoma in diffuse large b-cell lymphoma and their correlation with clinicopathological parameters
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Perubatan
publishDate 2017
url http://eprints.usm.my/45682/1/Dr.%20Wan%20Nor%20Najmiyah%20Wan%20Abdul%20Wahab-24%20pages.pdf
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