Production Of Crude Biopolymer From Bacteria In Drainage System

This work focuses on the extraction of biopolymer from bacteria in relation to wastewater treatment, specifically in flocculation process. Three isolates of bacterial strain (WIR, WRA, WIA) which are able to produce biopolymers as flocculants were identified from industrial and residential drainage...

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Main Author:	Talib, Azieda Abdul
Format:	Thesis
Language:	English
Published:	2014
Subjects:	T11.95-12.5 Industrial directories
Online Access:	http://eprints.usm.my/46051/1/Azieda%20Binti%20Abdul%20Talib24.pdf
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id	my-usm-ep.46051
record_format	uketd_dc
spelling	my-usm-ep.460512020-01-31T01:27:04Z Production Of Crude Biopolymer From Bacteria In Drainage System 2014-01 Talib, Azieda Abdul T11.95-12.5 Industrial directories This work focuses on the extraction of biopolymer from bacteria in relation to wastewater treatment, specifically in flocculation process. Three isolates of bacterial strain (WIR, WRA, WIA) which are able to produce biopolymers as flocculants were identified from industrial and residential drainage systems. These bacteria were identified as Bacillus megaterium BMRA, Bacillus megaterium BMIR and Bacillus subtilis BSIA through physiological characteristics (mucoid, slimy and ropy) and the partial sequences of their 16SrRNA. Crude biopolymers CWIR (industrial + residential) and CWRA (residential) were produced by Bacillus megaterium BMIR and BMRA while CWIA (industrial) was extracted from Bacillus subtilis BSIA. These bacteria produced their respective biopolymers at 40ºC given a two-day incubation period, with pH 7.0 ± 0.03. The crude biopolymers were recovered from the supernatant of the culture broth by ethanol precipitation and vacuum-dried in vacuum oven. CWIR, CWRA and CWIA contained 4.01%, 5.20% and 5.45% of protein respectively. Total sugar content in each biopolymer was 1.48% for CWIR, 1.65% for CWRA and 1.79% for CWIA. Elemental analyses showed that the CWIR obtained had 31.60% of C, 4.81% of H and 6.32% of N, while WRA contained 31.37% of C, 5.02% of H and 6.12% of N. WIA showed a composition comprising 31.25% of C, 4.88% of H and 5.69% of N. The molecular weight for each of the biopolymers CWIA, CWIR and CWRA was measured at 31.788 kDa, 55.344 kDa and 38.791 kDa respectively. 2014-01 Thesis http://eprints.usm.my/46051/ http://eprints.usm.my/46051/1/Azieda%20Binti%20Abdul%20Talib24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Teknologi Industri
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	T11.95-12.5 Industrial directories
spellingShingle	T11.95-12.5 Industrial directories Talib, Azieda Abdul Production Of Crude Biopolymer From Bacteria In Drainage System
description	This work focuses on the extraction of biopolymer from bacteria in relation to wastewater treatment, specifically in flocculation process. Three isolates of bacterial strain (WIR, WRA, WIA) which are able to produce biopolymers as flocculants were identified from industrial and residential drainage systems. These bacteria were identified as Bacillus megaterium BMRA, Bacillus megaterium BMIR and Bacillus subtilis BSIA through physiological characteristics (mucoid, slimy and ropy) and the partial sequences of their 16SrRNA. Crude biopolymers CWIR (industrial + residential) and CWRA (residential) were produced by Bacillus megaterium BMIR and BMRA while CWIA (industrial) was extracted from Bacillus subtilis BSIA. These bacteria produced their respective biopolymers at 40ºC given a two-day incubation period, with pH 7.0 ± 0.03. The crude biopolymers were recovered from the supernatant of the culture broth by ethanol precipitation and vacuum-dried in vacuum oven. CWIR, CWRA and CWIA contained 4.01%, 5.20% and 5.45% of protein respectively. Total sugar content in each biopolymer was 1.48% for CWIR, 1.65% for CWRA and 1.79% for CWIA. Elemental analyses showed that the CWIR obtained had 31.60% of C, 4.81% of H and 6.32% of N, while WRA contained 31.37% of C, 5.02% of H and 6.12% of N. WIA showed a composition comprising 31.25% of C, 4.88% of H and 5.69% of N. The molecular weight for each of the biopolymers CWIA, CWIR and CWRA was measured at 31.788 kDa, 55.344 kDa and 38.791 kDa respectively.
format	Thesis
qualification_level	Master's degree
author	Talib, Azieda Abdul
author_facet	Talib, Azieda Abdul
author_sort	Talib, Azieda Abdul
title	Production Of Crude Biopolymer From Bacteria In Drainage System
title_short	Production Of Crude Biopolymer From Bacteria In Drainage System
title_full	Production Of Crude Biopolymer From Bacteria In Drainage System
title_fullStr	Production Of Crude Biopolymer From Bacteria In Drainage System
title_full_unstemmed	Production Of Crude Biopolymer From Bacteria In Drainage System
title_sort	production of crude biopolymer from bacteria in drainage system
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Teknologi Industri
publishDate	2014
url	http://eprints.usm.my/46051/1/Azieda%20Binti%20Abdul%20Talib24.pdf
_version_	1747821600899596288

Production Of Crude Biopolymer From Bacteria In Drainage System

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