Molecular Epidemiology Of Salmonella Enterica Subspecies Enterica Serovar Typhi Isolates From Kelantan Using Random Amplified Polymorphic Dna And Single Nucleotide Polymorphism

Typhoid fever is an acute systemic infection of humans caused by Salmonella enterica subspecies enterica serovar Typhi (S. Typhi). Typhoid fever is endemic in Kelantan, a North-eastern state in Peninsular Malaysia. In spite of extensive intervention by the state‘s Public Health Department, outbreaks...

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Main Author:	Ja’afar, Ja’afar Nuhu
Format:	Thesis
Language:	English
Published:	2014
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/46114/1/Ja%E2%80%99afar%20Nuhu%20Ja%E2%80%99afar24.pdf
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id	my-usm-ep.46114
record_format	uketd_dc
spelling	my-usm-ep.461142020-02-06T08:44:33Z Molecular Epidemiology Of Salmonella Enterica Subspecies Enterica Serovar Typhi Isolates From Kelantan Using Random Amplified Polymorphic Dna And Single Nucleotide Polymorphism 2014-02 Ja’afar, Ja’afar Nuhu R5-920 Medicine (General) Typhoid fever is an acute systemic infection of humans caused by Salmonella enterica subspecies enterica serovar Typhi (S. Typhi). Typhoid fever is endemic in Kelantan, a North-eastern state in Peninsular Malaysia. In spite of extensive intervention by the state‘s Public Health Department, outbreaks of typhoid fever continue to occur with the emergence of multi-drug resistant strains. An important strategy to eradicate this persistent disease is to identify the sources and distribution of infection; and for this, a reliable S. Typhi fingerprinting method is needed. Confirmation of the bacteria by phenotypic techniques is difficult due to the vast number of serovars (>1,500) within the subspecies enterica. Current molecular typing methods offer very high discriminatory resolutions; however, the majority are limited in identifying phylogenetic relationships, evolutionary trends or genotypic characteristics. Thus, the main objective of this study was to investigate the effect of nucleotide sequence variations in S. Typhi in relation to its phylogenetic diversity, and to identify possible epidemiological markers for easy identification and confirmation of this pathogen. Two methods were used in this study. 2014-02 Thesis http://eprints.usm.my/46114/ http://eprints.usm.my/46114/1/Ja%E2%80%99afar%20Nuhu%20Ja%E2%80%99afar24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Ja’afar, Ja’afar Nuhu Molecular Epidemiology Of Salmonella Enterica Subspecies Enterica Serovar Typhi Isolates From Kelantan Using Random Amplified Polymorphic Dna And Single Nucleotide Polymorphism
description	Typhoid fever is an acute systemic infection of humans caused by Salmonella enterica subspecies enterica serovar Typhi (S. Typhi). Typhoid fever is endemic in Kelantan, a North-eastern state in Peninsular Malaysia. In spite of extensive intervention by the state‘s Public Health Department, outbreaks of typhoid fever continue to occur with the emergence of multi-drug resistant strains. An important strategy to eradicate this persistent disease is to identify the sources and distribution of infection; and for this, a reliable S. Typhi fingerprinting method is needed. Confirmation of the bacteria by phenotypic techniques is difficult due to the vast number of serovars (>1,500) within the subspecies enterica. Current molecular typing methods offer very high discriminatory resolutions; however, the majority are limited in identifying phylogenetic relationships, evolutionary trends or genotypic characteristics. Thus, the main objective of this study was to investigate the effect of nucleotide sequence variations in S. Typhi in relation to its phylogenetic diversity, and to identify possible epidemiological markers for easy identification and confirmation of this pathogen. Two methods were used in this study.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Ja’afar, Ja’afar Nuhu
author_facet	Ja’afar, Ja’afar Nuhu
author_sort	Ja’afar, Ja’afar Nuhu
title	Molecular Epidemiology Of Salmonella Enterica Subspecies Enterica Serovar Typhi Isolates From Kelantan Using Random Amplified Polymorphic Dna And Single Nucleotide Polymorphism
title_short	Molecular Epidemiology Of Salmonella Enterica Subspecies Enterica Serovar Typhi Isolates From Kelantan Using Random Amplified Polymorphic Dna And Single Nucleotide Polymorphism
title_full	Molecular Epidemiology Of Salmonella Enterica Subspecies Enterica Serovar Typhi Isolates From Kelantan Using Random Amplified Polymorphic Dna And Single Nucleotide Polymorphism
title_fullStr	Molecular Epidemiology Of Salmonella Enterica Subspecies Enterica Serovar Typhi Isolates From Kelantan Using Random Amplified Polymorphic Dna And Single Nucleotide Polymorphism
title_full_unstemmed	Molecular Epidemiology Of Salmonella Enterica Subspecies Enterica Serovar Typhi Isolates From Kelantan Using Random Amplified Polymorphic Dna And Single Nucleotide Polymorphism
title_sort	molecular epidemiology of salmonella enterica subspecies enterica serovar typhi isolates from kelantan using random amplified polymorphic dna and single nucleotide polymorphism
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Perubatan Molekul
publishDate	2014
url	http://eprints.usm.my/46114/1/Ja%E2%80%99afar%20Nuhu%20Ja%E2%80%99afar24.pdf
_version_	1747821613603094528

Molecular Epidemiology Of Salmonella Enterica Subspecies Enterica Serovar Typhi Isolates From Kelantan Using Random Amplified Polymorphic Dna And Single Nucleotide Polymorphism

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