Integration Of Glutaraldehyde Onto Immuno-Membrane For Polyaniline-Based Electrochemical Biosensor

Development of membrane for bio-sensing applications for epidemics control has a huge global impact especially for public health. This research explored the electrochemical biosensor with polyaniline (PANI) as a transducer through analyzing the biotin-bovine serum albumin (BSA) interaction as a mode...

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Main Author:	Shaimi, Roswani
Format:	Thesis
Language:	English
Published:	2013
Subjects:	TP1-1185 Chemical technology
Online Access:	http://eprints.usm.my/46206/1/Roswani%20Binti%20Shaimi24.pdf
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spelling	my-usm-ep.462062020-02-13T06:16:11Z Integration Of Glutaraldehyde Onto Immuno-Membrane For Polyaniline-Based Electrochemical Biosensor 2013-12 Shaimi, Roswani TP1-1185 Chemical technology Development of membrane for bio-sensing applications for epidemics control has a huge global impact especially for public health. This research explored the electrochemical biosensor with polyaniline (PANI) as a transducer through analyzing the biotin-bovine serum albumin (BSA) interaction as a model system. As membrane appeared as the platform for protein immobilization, selection of suitable membrane is required to create a sensitive and specific biosensor. In measurement of membrane performance, membrane protein binding abilities were quantitatively determined. Among the tested membranes, NC-180 appeared as the most suitable lateral flow membrane as it performed high protein binding ability and short lateral wicking time. To increase the membrane protein binding ability, NC-180 was then subjected to membrane modification using glutaraldehyde (GA) solution. The protein binding of the modified membrane with GA was able to retain up to temperature of 60oC. In the present study, the effect of various integration factors such as the concentration of GA, integration time and number of the integration layer on membrane‟s protein binding ability was investigated. The optimum integration conditions were determined using response surface methodology (RSM). 2013-12 Thesis http://eprints.usm.my/46206/ http://eprints.usm.my/46206/1/Roswani%20Binti%20Shaimi24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Kejuruteraan Kimia
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	TP1-1185 Chemical technology
spellingShingle	TP1-1185 Chemical technology Shaimi, Roswani Integration Of Glutaraldehyde Onto Immuno-Membrane For Polyaniline-Based Electrochemical Biosensor
description	Development of membrane for bio-sensing applications for epidemics control has a huge global impact especially for public health. This research explored the electrochemical biosensor with polyaniline (PANI) as a transducer through analyzing the biotin-bovine serum albumin (BSA) interaction as a model system. As membrane appeared as the platform for protein immobilization, selection of suitable membrane is required to create a sensitive and specific biosensor. In measurement of membrane performance, membrane protein binding abilities were quantitatively determined. Among the tested membranes, NC-180 appeared as the most suitable lateral flow membrane as it performed high protein binding ability and short lateral wicking time. To increase the membrane protein binding ability, NC-180 was then subjected to membrane modification using glutaraldehyde (GA) solution. The protein binding of the modified membrane with GA was able to retain up to temperature of 60oC. In the present study, the effect of various integration factors such as the concentration of GA, integration time and number of the integration layer on membrane‟s protein binding ability was investigated. The optimum integration conditions were determined using response surface methodology (RSM).
format	Thesis
qualification_level	Master's degree
author	Shaimi, Roswani
author_facet	Shaimi, Roswani
author_sort	Shaimi, Roswani
title	Integration Of Glutaraldehyde Onto Immuno-Membrane For Polyaniline-Based Electrochemical Biosensor
title_short	Integration Of Glutaraldehyde Onto Immuno-Membrane For Polyaniline-Based Electrochemical Biosensor
title_full	Integration Of Glutaraldehyde Onto Immuno-Membrane For Polyaniline-Based Electrochemical Biosensor
title_fullStr	Integration Of Glutaraldehyde Onto Immuno-Membrane For Polyaniline-Based Electrochemical Biosensor
title_full_unstemmed	Integration Of Glutaraldehyde Onto Immuno-Membrane For Polyaniline-Based Electrochemical Biosensor
title_sort	integration of glutaraldehyde onto immuno-membrane for polyaniline-based electrochemical biosensor
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Kejuruteraan Kimia
publishDate	2013
url	http://eprints.usm.my/46206/1/Roswani%20Binti%20Shaimi24.pdf
_version_	1747821631883968512

Integration Of Glutaraldehyde Onto Immuno-Membrane For Polyaniline-Based Electrochemical Biosensor

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