Non-Protein Coding RNA Genes As The Potential Markers For The Detection And Differentiation Of Salmonella Enterica Serovar Typhi And Salmonella Enterica Serovar Paratyphi A Using PCR

Non-protein-coding RNAs (npcRNAs) are not translated into protein but are key regulatory molecules in the cell. Recently, the expression of specific npcRNAs has been linked to various human diseases and cancer. Salmonellosis is a communicable disease caused by members of the Salmonella species, whic...

Full description

Saved in:

Bibliographic Details
Main Author:	Ravichantar, Nithya
Format:	Thesis
Language:	English
Published:	2014
Subjects:	R Medicine (General)
Online Access:	http://eprints.usm.my/46409/1/NITHYA%20RAVICHANTAR_HJ.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.46409
record_format	uketd_dc
spelling	my-usm-ep.464092020-03-02T03:11:27Z Non-Protein Coding RNA Genes As The Potential Markers For The Detection And Differentiation Of Salmonella Enterica Serovar Typhi And Salmonella Enterica Serovar Paratyphi A Using PCR 2014-09 Ravichantar, Nithya R Medicine (General) Non-protein-coding RNAs (npcRNAs) are not translated into protein but are key regulatory molecules in the cell. Recently, the expression of specific npcRNAs has been linked to various human diseases and cancer. Salmonellosis is a communicable disease caused by members of the Salmonella species, which infect humans through contaminated food or water. It is of paramount importance that accurate detection methods are available for differentiating the Salmonella serovars that produce severe infection in humans, including S. Typhi and S. Paratyphi A. The efficacy of using npcRNA genes from S. Typhi as molecular targets in detection and differentiation of S. Typhi and S.Paratyphi A using a a multiplex polymerase chain reaction (mPCR) assay was reported in this study. The detection limit for this mPCR assay was 10pg of gDNA. Moreover, in a stool-seeding experiment with S. Typhi and S. Paratyphi A, a respective detection limit of 1.5 x 101 and 1.5 x 100 CFU/mL following 4h of enrichment in selenite cystine broth offers an early clinical diagnostics. Collectively, this study has successfully developed npcRNA genes based mPCR that is able to detect and differentiate S. Typhi, S. Paratyphi A in a typhoid infection besides discriminating them from a general salmonellosis. With its comparable specificity and sensitivity level to other studies/ products, this mPCR could prove to be an excellent alternative for clinical diagnosis and disease monitoring of salmonellosis. 2014-09 Thesis http://eprints.usm.my/46409/ http://eprints.usm.my/46409/1/NITHYA%20RAVICHANTAR_HJ.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Ravichantar, Nithya Non-Protein Coding RNA Genes As The Potential Markers For The Detection And Differentiation Of Salmonella Enterica Serovar Typhi And Salmonella Enterica Serovar Paratyphi A Using PCR
description	Non-protein-coding RNAs (npcRNAs) are not translated into protein but are key regulatory molecules in the cell. Recently, the expression of specific npcRNAs has been linked to various human diseases and cancer. Salmonellosis is a communicable disease caused by members of the Salmonella species, which infect humans through contaminated food or water. It is of paramount importance that accurate detection methods are available for differentiating the Salmonella serovars that produce severe infection in humans, including S. Typhi and S. Paratyphi A. The efficacy of using npcRNA genes from S. Typhi as molecular targets in detection and differentiation of S. Typhi and S.Paratyphi A using a a multiplex polymerase chain reaction (mPCR) assay was reported in this study. The detection limit for this mPCR assay was 10pg of gDNA. Moreover, in a stool-seeding experiment with S. Typhi and S. Paratyphi A, a respective detection limit of 1.5 x 101 and 1.5 x 100 CFU/mL following 4h of enrichment in selenite cystine broth offers an early clinical diagnostics. Collectively, this study has successfully developed npcRNA genes based mPCR that is able to detect and differentiate S. Typhi, S. Paratyphi A in a typhoid infection besides discriminating them from a general salmonellosis. With its comparable specificity and sensitivity level to other studies/ products, this mPCR could prove to be an excellent alternative for clinical diagnosis and disease monitoring of salmonellosis.
format	Thesis
qualification_level	Master's degree
author	Ravichantar, Nithya
author_facet	Ravichantar, Nithya
author_sort	Ravichantar, Nithya
title	Non-Protein Coding RNA Genes As The Potential Markers For The Detection And Differentiation Of Salmonella Enterica Serovar Typhi And Salmonella Enterica Serovar Paratyphi A Using PCR
title_short	Non-Protein Coding RNA Genes As The Potential Markers For The Detection And Differentiation Of Salmonella Enterica Serovar Typhi And Salmonella Enterica Serovar Paratyphi A Using PCR
title_full	Non-Protein Coding RNA Genes As The Potential Markers For The Detection And Differentiation Of Salmonella Enterica Serovar Typhi And Salmonella Enterica Serovar Paratyphi A Using PCR
title_fullStr	Non-Protein Coding RNA Genes As The Potential Markers For The Detection And Differentiation Of Salmonella Enterica Serovar Typhi And Salmonella Enterica Serovar Paratyphi A Using PCR
title_full_unstemmed	Non-Protein Coding RNA Genes As The Potential Markers For The Detection And Differentiation Of Salmonella Enterica Serovar Typhi And Salmonella Enterica Serovar Paratyphi A Using PCR
title_sort	non-protein coding rna genes as the potential markers for the detection and differentiation of salmonella enterica serovar typhi and salmonella enterica serovar paratyphi a using pcr
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2014
url	http://eprints.usm.my/46409/1/NITHYA%20RAVICHANTAR_HJ.pdf
_version_	1747821664151797760

Non-Protein Coding RNA Genes As The Potential Markers For The Detection And Differentiation Of Salmonella Enterica Serovar Typhi And Salmonella Enterica Serovar Paratyphi A Using PCR

Similar Items