Identification And Characterization Of Fusarium Species From Highlands In Malaysia

Fusarium is one of the heterogeneous genera of saprophytic, pathogenic, and toxigenic fungi distributed worldwide. However, such an important genus is considered to be one of the most highly diversified, thus difficult to identify and classify. Recently, several Fusarium species normally found in th...

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Main Author:	Manshor, Nurhazrati
Format:	Thesis
Language:	English
Published:	2014
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/46451/1/Nurhazrati%20Binti%20Manshor24.pdf
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id	my-usm-ep.46451
record_format	uketd_dc
spelling	my-usm-ep.464512020-03-06T03:09:11Z Identification And Characterization Of Fusarium Species From Highlands In Malaysia 2014-02 Manshor, Nurhazrati QH1-278.5 Natural history (General) Fusarium is one of the heterogeneous genera of saprophytic, pathogenic, and toxigenic fungi distributed worldwide. However, such an important genus is considered to be one of the most highly diversified, thus difficult to identify and classify. Recently, several Fusarium species normally found in the temperate and subtropical regions were also reported to be in the highlands of tropical areas, including Malaysia. This research was to identify Fusarium species in context of occurrence, diversity, and toxigenicity. Phase sampling was done randomly from variable hosts and substrate in three locations, Cameron Highlands, Fraser’s Hill and Kinabalu Park. The samples were plated on a semi-selective medium (pentachloronitrobenzene agar) and incubated in a standard incubation conditions (27+1°C). One-hundred twenty three (123) Fusarium isolates were single-spored and identified based on the morphological characteristics. Four species were identified i.e. F. graminearum (86.2%), followed by F. venenatum (12.2%), F. avenaceum (0.8%) and F. sporotrichioides (0.8%). Molecular characterization and mycotoxin profiles were used to support the morphological data. The molecular characterization were carried out by using PCR-RFLP of the rDNA intergenic spacer (IGS) region and DNA sequences using Translation α-Elongation Factor (TEF) gene. BLAST search for sequence similarity by using GenBank database which demonstrated 88% - 99% whereas for Fusarium Database was between 89% - 99%. 2014-02 Thesis http://eprints.usm.my/46451/ http://eprints.usm.my/46451/1/Nurhazrati%20Binti%20Manshor24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Manshor, Nurhazrati Identification And Characterization Of Fusarium Species From Highlands In Malaysia
description	Fusarium is one of the heterogeneous genera of saprophytic, pathogenic, and toxigenic fungi distributed worldwide. However, such an important genus is considered to be one of the most highly diversified, thus difficult to identify and classify. Recently, several Fusarium species normally found in the temperate and subtropical regions were also reported to be in the highlands of tropical areas, including Malaysia. This research was to identify Fusarium species in context of occurrence, diversity, and toxigenicity. Phase sampling was done randomly from variable hosts and substrate in three locations, Cameron Highlands, Fraser’s Hill and Kinabalu Park. The samples were plated on a semi-selective medium (pentachloronitrobenzene agar) and incubated in a standard incubation conditions (27+1°C). One-hundred twenty three (123) Fusarium isolates were single-spored and identified based on the morphological characteristics. Four species were identified i.e. F. graminearum (86.2%), followed by F. venenatum (12.2%), F. avenaceum (0.8%) and F. sporotrichioides (0.8%). Molecular characterization and mycotoxin profiles were used to support the morphological data. The molecular characterization were carried out by using PCR-RFLP of the rDNA intergenic spacer (IGS) region and DNA sequences using Translation α-Elongation Factor (TEF) gene. BLAST search for sequence similarity by using GenBank database which demonstrated 88% - 99% whereas for Fusarium Database was between 89% - 99%.
format	Thesis
qualification_level	Master's degree
author	Manshor, Nurhazrati
author_facet	Manshor, Nurhazrati
author_sort	Manshor, Nurhazrati
title	Identification And Characterization Of Fusarium Species From Highlands In Malaysia
title_short	Identification And Characterization Of Fusarium Species From Highlands In Malaysia
title_full	Identification And Characterization Of Fusarium Species From Highlands In Malaysia
title_fullStr	Identification And Characterization Of Fusarium Species From Highlands In Malaysia
title_full_unstemmed	Identification And Characterization Of Fusarium Species From Highlands In Malaysia
title_sort	identification and characterization of fusarium species from highlands in malaysia
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2014
url	http://eprints.usm.my/46451/1/Nurhazrati%20Binti%20Manshor24.pdf
_version_	1747821673694887936

Identification And Characterization Of Fusarium Species From Highlands In Malaysia

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