Characterization And Ochratoxin ‘A’ Production Of Aspergillus Section Nigri

Several species of black Aspergillus or Aspergillus section Nigri can act as saprophytes as well as contaminants of food and feed. There are also black Aspergillus that are toxigenic, producing ochratoxin A (OTA) which is a mycotoxin known to be associated with food and feed. This toxin can cause ad...

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Main Author:	Rahman, Wardah Abdul
Format:	Thesis
Language:	English
Published:	2019
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/46683/1/Wardah%20binti%20Abdul%20Rahman%20CHARACTERIZATION%20AND%20OCHRATOXIN%20%E2%80%98A%E2%80%99%20%20PRODUCTION%20OF%20Aspergillus%20SECTION%20Nigri%202019%20PhD24.pdf
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spelling	my-usm-ep.466832020-07-03T08:04:17Z Characterization And Ochratoxin ‘A’ Production Of Aspergillus Section Nigri 2019-04 Rahman, Wardah Abdul QH1-278.5 Natural history (General) Several species of black Aspergillus or Aspergillus section Nigri can act as saprophytes as well as contaminants of food and feed. There are also black Aspergillus that are toxigenic, producing ochratoxin A (OTA) which is a mycotoxin known to be associated with food and feed. This toxin can cause adverse effects to humans and animals. The aims of this present study were to accurately identified and characterize black Aspergillus from various substrates and indoor environment in Malaysia, to determine the phylogenetic relationships between the species, to characterize the species using Restriction Fragment Length Polymorphism (RFLP) technique of internal transcribed spacer regions (ITS) and to determine the ability of the species to produce OTA. In this study, 177 isolates of black Aspergillus from food (rice, groundnuts and spices), feed (corn grains), soil and indoor environment were morphologically identified as A. niger (153 isolates) and A. aculeatus (24 isolates). Based on sequences of ITS, β-tubulin and calmodulin, the species were re-identified as A. niger (144 isolates), A. aculeatus (24 isolates) and A. tubingensis (nine isolates). Phylogenetic analysis of individual and combined ITS, β-tubulin and calmodulin sequences using Neighbour-joining and Maximum Likelihood methods showed that A. niger, A. aculeatus and A. tubingensis were clustered according to the species. 2019-04 Thesis http://eprints.usm.my/46683/ http://eprints.usm.my/46683/1/Wardah%20binti%20Abdul%20Rahman%20CHARACTERIZATION%20AND%20OCHRATOXIN%20%E2%80%98A%E2%80%99%20%20PRODUCTION%20OF%20Aspergillus%20SECTION%20Nigri%202019%20PhD24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Rahman, Wardah Abdul Characterization And Ochratoxin ‘A’ Production Of Aspergillus Section Nigri
description	Several species of black Aspergillus or Aspergillus section Nigri can act as saprophytes as well as contaminants of food and feed. There are also black Aspergillus that are toxigenic, producing ochratoxin A (OTA) which is a mycotoxin known to be associated with food and feed. This toxin can cause adverse effects to humans and animals. The aims of this present study were to accurately identified and characterize black Aspergillus from various substrates and indoor environment in Malaysia, to determine the phylogenetic relationships between the species, to characterize the species using Restriction Fragment Length Polymorphism (RFLP) technique of internal transcribed spacer regions (ITS) and to determine the ability of the species to produce OTA. In this study, 177 isolates of black Aspergillus from food (rice, groundnuts and spices), feed (corn grains), soil and indoor environment were morphologically identified as A. niger (153 isolates) and A. aculeatus (24 isolates). Based on sequences of ITS, β-tubulin and calmodulin, the species were re-identified as A. niger (144 isolates), A. aculeatus (24 isolates) and A. tubingensis (nine isolates). Phylogenetic analysis of individual and combined ITS, β-tubulin and calmodulin sequences using Neighbour-joining and Maximum Likelihood methods showed that A. niger, A. aculeatus and A. tubingensis were clustered according to the species.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Rahman, Wardah Abdul
author_facet	Rahman, Wardah Abdul
author_sort	Rahman, Wardah Abdul
title	Characterization And Ochratoxin ‘A’ Production Of Aspergillus Section Nigri
title_short	Characterization And Ochratoxin ‘A’ Production Of Aspergillus Section Nigri
title_full	Characterization And Ochratoxin ‘A’ Production Of Aspergillus Section Nigri
title_fullStr	Characterization And Ochratoxin ‘A’ Production Of Aspergillus Section Nigri
title_full_unstemmed	Characterization And Ochratoxin ‘A’ Production Of Aspergillus Section Nigri
title_sort	characterization and ochratoxin ‘a’ production of aspergillus section nigri
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2019
url	http://eprints.usm.my/46683/1/Wardah%20binti%20Abdul%20Rahman%20CHARACTERIZATION%20AND%20OCHRATOXIN%20%E2%80%98A%E2%80%99%20%20PRODUCTION%20OF%20Aspergillus%20SECTION%20Nigri%202019%20PhD24.pdf
_version_	1747821712093741056

Characterization And Ochratoxin ‘A’ Production Of Aspergillus Section Nigri

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