The role of alpha-giardin in response to drug treatment in giardia intestinalis

Giardiasis, which is caused by the intestinal protozoan parasite Giardia intestinalis, affects approximately 280 million people around the world, particularly in children below five years of age. Oral ingestion of as few as ten cysts of G. intestinalis can cause infection in human which lead to comp...

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Main Author:	Roslim, Nurul Afiedia
Format:	Thesis
Language:	English
Published:	2018
Subjects:	RA643-645 Disease (Communicable and noninfectious) and public health
Online Access:	http://eprints.usm.my/47462/1/Dr.%20Nurul%20Afiedia%20Roslim-24%20pages.pdf
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id	my-usm-ep.47462
record_format	uketd_dc
spelling	my-usm-ep.474622020-10-08T05:35:12Z The role of alpha-giardin in response to drug treatment in giardia intestinalis 2018 Roslim, Nurul Afiedia RA643-645 Disease (Communicable and noninfectious) and public health Giardiasis, which is caused by the intestinal protozoan parasite Giardia intestinalis, affects approximately 280 million people around the world, particularly in children below five years of age. Oral ingestion of as few as ten cysts of G. intestinalis can cause infection in human which lead to complications such as severe dehydration and weight loss. There are several antigiardial drugs available for giardiasis treatment; however, issues such as drug resistance, reinfection and recurrence of symptoms have been highly reported. Thus, the finding for a novel potential drug targets is important to control giardiasis. In this study, we sought to examine the role of alpha-giardins in response to drug treatment in G. intestinalis. Our results showed that G. intestinalis were more susceptible to the action of Mebendazole (MBZ) as the IC50 value for MBZ was 0.06 μM when compared with Metronidazole (MTZ) of 9.177 μM. Trophozoites treated with high concentration of MBZ or MTZ showed significant reduction in viability. The release of reactive oxygen species were not detected in either MBZ or MTZ-treated G. intestinalis. We also found that G. intestinalis treated with 100 μM MBZ or MTZ showed significant upregulated expression of alpha-2 giardin (p ≤ 0.05). In conclusion, alpha-2 giardin could serve as a novel potential target for future drug design and vaccine development. 2018 Thesis http://eprints.usm.my/47462/ http://eprints.usm.my/47462/1/Dr.%20Nurul%20Afiedia%20Roslim-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RA643-645 Disease (Communicable and noninfectious) and public health
spellingShingle	RA643-645 Disease (Communicable and noninfectious) and public health Roslim, Nurul Afiedia The role of alpha-giardin in response to drug treatment in giardia intestinalis
description	Giardiasis, which is caused by the intestinal protozoan parasite Giardia intestinalis, affects approximately 280 million people around the world, particularly in children below five years of age. Oral ingestion of as few as ten cysts of G. intestinalis can cause infection in human which lead to complications such as severe dehydration and weight loss. There are several antigiardial drugs available for giardiasis treatment; however, issues such as drug resistance, reinfection and recurrence of symptoms have been highly reported. Thus, the finding for a novel potential drug targets is important to control giardiasis. In this study, we sought to examine the role of alpha-giardins in response to drug treatment in G. intestinalis. Our results showed that G. intestinalis were more susceptible to the action of Mebendazole (MBZ) as the IC50 value for MBZ was 0.06 μM when compared with Metronidazole (MTZ) of 9.177 μM. Trophozoites treated with high concentration of MBZ or MTZ showed significant reduction in viability. The release of reactive oxygen species were not detected in either MBZ or MTZ-treated G. intestinalis. We also found that G. intestinalis treated with 100 μM MBZ or MTZ showed significant upregulated expression of alpha-2 giardin (p ≤ 0.05). In conclusion, alpha-2 giardin could serve as a novel potential target for future drug design and vaccine development.
format	Thesis
qualification_level	Master's degree
author	Roslim, Nurul Afiedia
author_facet	Roslim, Nurul Afiedia
author_sort	Roslim, Nurul Afiedia
title	The role of alpha-giardin in response to drug treatment in giardia intestinalis
title_short	The role of alpha-giardin in response to drug treatment in giardia intestinalis
title_full	The role of alpha-giardin in response to drug treatment in giardia intestinalis
title_fullStr	The role of alpha-giardin in response to drug treatment in giardia intestinalis
title_full_unstemmed	The role of alpha-giardin in response to drug treatment in giardia intestinalis
title_sort	role of alpha-giardin in response to drug treatment in giardia intestinalis
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2018
url	http://eprints.usm.my/47462/1/Dr.%20Nurul%20Afiedia%20Roslim-24%20pages.pdf
_version_	1747821782372450304

The role of alpha-giardin in response to drug treatment in giardia intestinalis

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