Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities

The use of plant to synthesise silver nanoparticles (AgNPs) is considered as a green technology. In this study, a non-toxic, cost effective and eco-friendly method has been applied to synthesise AgNPs using aqueous extract of leaf and stem of Clinacanthus nutans (C. nutans). The synthesised AgNPs we...

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Main Author: Che Mood, Che Nurul Azieyan
Format: Thesis
Language:English
Published: 2018
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Online Access:http://eprints.usm.my/47922/1/CHE%20NURUL%20AZIEYAN%20-%20SILVER%20NANOPARTICLES%20OF%20CLINACANTHUS.pdf
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spelling my-usm-ep.479222020-11-20T03:23:35Z Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities 2018-07 Che Mood, Che Nurul Azieyan RB37-56.5 Clinical pathology. Laboratory technique The use of plant to synthesise silver nanoparticles (AgNPs) is considered as a green technology. In this study, a non-toxic, cost effective and eco-friendly method has been applied to synthesise AgNPs using aqueous extract of leaf and stem of Clinacanthus nutans (C. nutans). The synthesised AgNPs were tested on antimicrobial, antioxidant and cytotoxicity activities. The formation of AgNPs was confirmed by UV-Vis spectroscopy at the wavelength of 420 nm (AgNP-L) and 430 nm (AgNP-S). The size were measured at 114 nm (AgNP-L) and 129 nm (AgNP-S) using a zetasizer. From TEM analysis, the size ranged from 10-300 nm and 10-180 nm for AgNP-L and AgNP-S with spherical shape. Zeta potentials recorded at -42.8 mV for AgNP-L and -43.9 mV for AgNP-S. XRD analysis matched the face-centered cubic (fcc) structure of silver. FTIR analysis revealed the presence of few functional groups of phenolic and flavonoid compounds which acts as the reducing agent in AgNPs synthesis. The AgNPs showed good inhibition against bacteria, but not fungus. Inhibition showed that highest against S. aureus with 11.35 mm (AgNP-L) and 11.52 mm (AgNP-S), while the lowest inhibition against E. coli with 9.22 mm (AgNP-L) and 9.25 mm (AgNP-S) in disc diffusion method. Same trend of result was reported for well diffusion method. In antioxidant assays, AgNP-L showed IC50 at 417.05 μg/mL and AgNP-S at 434.60 μg/mL (DPPH); 304.31 μg/mL (AgNP-L) and 326.83 μg/mL (AgNP-S) for ABTS, respectively. 2018-07 Thesis http://eprints.usm.my/47922/ http://eprints.usm.my/47922/1/CHE%20NURUL%20AZIEYAN%20-%20SILVER%20NANOPARTICLES%20OF%20CLINACANTHUS.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic RB37-56.5 Clinical pathology
Laboratory technique
spellingShingle RB37-56.5 Clinical pathology
Laboratory technique
Che Mood, Che Nurul Azieyan
Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities
description The use of plant to synthesise silver nanoparticles (AgNPs) is considered as a green technology. In this study, a non-toxic, cost effective and eco-friendly method has been applied to synthesise AgNPs using aqueous extract of leaf and stem of Clinacanthus nutans (C. nutans). The synthesised AgNPs were tested on antimicrobial, antioxidant and cytotoxicity activities. The formation of AgNPs was confirmed by UV-Vis spectroscopy at the wavelength of 420 nm (AgNP-L) and 430 nm (AgNP-S). The size were measured at 114 nm (AgNP-L) and 129 nm (AgNP-S) using a zetasizer. From TEM analysis, the size ranged from 10-300 nm and 10-180 nm for AgNP-L and AgNP-S with spherical shape. Zeta potentials recorded at -42.8 mV for AgNP-L and -43.9 mV for AgNP-S. XRD analysis matched the face-centered cubic (fcc) structure of silver. FTIR analysis revealed the presence of few functional groups of phenolic and flavonoid compounds which acts as the reducing agent in AgNPs synthesis. The AgNPs showed good inhibition against bacteria, but not fungus. Inhibition showed that highest against S. aureus with 11.35 mm (AgNP-L) and 11.52 mm (AgNP-S), while the lowest inhibition against E. coli with 9.22 mm (AgNP-L) and 9.25 mm (AgNP-S) in disc diffusion method. Same trend of result was reported for well diffusion method. In antioxidant assays, AgNP-L showed IC50 at 417.05 μg/mL and AgNP-S at 434.60 μg/mL (DPPH); 304.31 μg/mL (AgNP-L) and 326.83 μg/mL (AgNP-S) for ABTS, respectively.
format Thesis
qualification_level Master's degree
author Che Mood, Che Nurul Azieyan
author_facet Che Mood, Che Nurul Azieyan
author_sort Che Mood, Che Nurul Azieyan
title Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities
title_short Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities
title_full Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities
title_fullStr Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities
title_full_unstemmed Silver Nanoparticles Of Clinacanthus Nutans Aqueous Extract: Biosynthesis, Phytochemical And Selected Biological Activities
title_sort silver nanoparticles of clinacanthus nutans aqueous extract: biosynthesis, phytochemical and selected biological activities
granting_institution Universiti Sains Malaysia
granting_department Institut Perubatan & Pergigian Termaju
publishDate 2018
url http://eprints.usm.my/47922/1/CHE%20NURUL%20AZIEYAN%20-%20SILVER%20NANOPARTICLES%20OF%20CLINACANTHUS.pdf
_version_ 1747821849288376320