Genetic And Chemical Variation Of Clinacanthus Nutans From Northern Region Of Peninsular Malaysia

Clinacanthus nutans is a valuable medicinal plant which has gained more attention in the last few years mainly because of its pharmacological properties. Despite this, there is little information available about the genetic and phytochemicals of the plant in different locations. Therefore, the objec...

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Main Author:	Ismail, Noor Zafirah
Format:	Thesis
Language:	English
Published:	2018
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/47942/1/NOOR%20ZAFIRAH%20BINTI%20ISMAIL%20-%20GENETIC%20DIVERSITY%20AND%20CHEMICAL%20PROFILING%20OF%20CLINACANTHUS%20NUTANS.pdf
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spelling	my-usm-ep.479422020-11-27T02:20:41Z Genetic And Chemical Variation Of Clinacanthus Nutans From Northern Region Of Peninsular Malaysia 2018-07 Ismail, Noor Zafirah R5-920 Medicine (General) Clinacanthus nutans is a valuable medicinal plant which has gained more attention in the last few years mainly because of its pharmacological properties. Despite this, there is little information available about the genetic and phytochemicals of the plant in different locations. Therefore, the objectives of this study were to identify C. nutans using deoxyribonucleic acid (DNA) barcode loci, evaluate genetic diversity by using 17 primers of ‘random amplified polymorphic deoxyribonucleic acids’ (RAPD), eight primers of ‘inter-simple sequence repeats’ (ISSR) and 136 primers of ‘random amplified microsatellite polymorphisms’ (RAMP) and determine the total flavonoid, phenolic contents, antioxidant activity and phytochemical contents of C. nutans using gas chromatography-mass spectrometry (GC-MS). A total of 80 C. nutans accessions from eight different locations in the northern region of Peninsular Malaysia were harvested. The plant was identified using voucher collection and the sequence of polymerase chain reaction (PCR) products using DNA barcode markers namely matK, rbcL, and trnH-psbA. The PCR product sequence analysis showed that C. nutans was identified and trnH-psbA was chosen as the suitable marker for C. nutans identification. Genomic DNA had successfully amplified ten primers of RAPD, five primers of ISSR and 37 primers of RAMP by using PCR in genetic variation analysis. C. nutans showed low percentage polymorphism at the population level compare to species level. 2018-07 Thesis http://eprints.usm.my/47942/ http://eprints.usm.my/47942/1/NOOR%20ZAFIRAH%20BINTI%20ISMAIL%20-%20GENETIC%20DIVERSITY%20AND%20CHEMICAL%20PROFILING%20OF%20CLINACANTHUS%20NUTANS.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Ismail, Noor Zafirah Genetic And Chemical Variation Of Clinacanthus Nutans From Northern Region Of Peninsular Malaysia
description	Clinacanthus nutans is a valuable medicinal plant which has gained more attention in the last few years mainly because of its pharmacological properties. Despite this, there is little information available about the genetic and phytochemicals of the plant in different locations. Therefore, the objectives of this study were to identify C. nutans using deoxyribonucleic acid (DNA) barcode loci, evaluate genetic diversity by using 17 primers of ‘random amplified polymorphic deoxyribonucleic acids’ (RAPD), eight primers of ‘inter-simple sequence repeats’ (ISSR) and 136 primers of ‘random amplified microsatellite polymorphisms’ (RAMP) and determine the total flavonoid, phenolic contents, antioxidant activity and phytochemical contents of C. nutans using gas chromatography-mass spectrometry (GC-MS). A total of 80 C. nutans accessions from eight different locations in the northern region of Peninsular Malaysia were harvested. The plant was identified using voucher collection and the sequence of polymerase chain reaction (PCR) products using DNA barcode markers namely matK, rbcL, and trnH-psbA. The PCR product sequence analysis showed that C. nutans was identified and trnH-psbA was chosen as the suitable marker for C. nutans identification. Genomic DNA had successfully amplified ten primers of RAPD, five primers of ISSR and 37 primers of RAMP by using PCR in genetic variation analysis. C. nutans showed low percentage polymorphism at the population level compare to species level.
format	Thesis
qualification_level	Master's degree
author	Ismail, Noor Zafirah
author_facet	Ismail, Noor Zafirah
author_sort	Ismail, Noor Zafirah
title	Genetic And Chemical Variation Of Clinacanthus Nutans From Northern Region Of Peninsular Malaysia
title_short	Genetic And Chemical Variation Of Clinacanthus Nutans From Northern Region Of Peninsular Malaysia
title_full	Genetic And Chemical Variation Of Clinacanthus Nutans From Northern Region Of Peninsular Malaysia
title_fullStr	Genetic And Chemical Variation Of Clinacanthus Nutans From Northern Region Of Peninsular Malaysia
title_full_unstemmed	Genetic And Chemical Variation Of Clinacanthus Nutans From Northern Region Of Peninsular Malaysia
title_sort	genetic and chemical variation of clinacanthus nutans from northern region of peninsular malaysia
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2018
url	http://eprints.usm.my/47942/1/NOOR%20ZAFIRAH%20BINTI%20ISMAIL%20-%20GENETIC%20DIVERSITY%20AND%20CHEMICAL%20PROFILING%20OF%20CLINACANTHUS%20NUTANS.pdf
_version_	1747821853434445824

Genetic And Chemical Variation Of Clinacanthus Nutans From Northern Region Of Peninsular Malaysia

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