Development Of Species-Specific Microsatellite Markers Of Termite Globitermes Sulphurues Haviland (Blattodea: Termitidae) For Genetic Population Analysis By Using Next-Generation Sequencing (Ngs) Approach
Subterranean termite Globitermes sulphureus (higher termite) comprise a wide distributed family of termites, found throughout the tropical regions of the world. Little is known about G. sulphureus role in biology, dispersal range, and their control due to lack of research to gather molecular data...
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my-usm-ep.482282021-02-02T03:39:17Z Development Of Species-Specific Microsatellite Markers Of Termite Globitermes Sulphurues Haviland (Blattodea: Termitidae) For Genetic Population Analysis By Using Next-Generation Sequencing (Ngs) Approach 2019-06 Khizam, Nur Aizatul Nathasha QH301 Biology Subterranean termite Globitermes sulphureus (higher termite) comprise a wide distributed family of termites, found throughout the tropical regions of the world. Little is known about G. sulphureus role in biology, dispersal range, and their control due to lack of research to gather molecular data information. Hence, from the availability of whole sequences of G. sulphureus, a great opportunity to develop species-specific microsatellite markers can be done. In this study, a total of 243,057 transcripts were generated from transcriptome dataset of G. sulphureus, with dinucleotide repeats being the most abundant. From these, a total of new 30 species-specific microsatellite markers were designed using next-generation sequencing (NGS) procedures. All of 30 markers were successfully amplified and showed visible scorable polymorphism. The polymorphic information content (PIC) values showed greater than 0.5 in which proved that all markers here had high level of polymorphism, with 3 to 9 alleles per locus were identified. The observed and expected heterozygosities ranged from 0 to 0.83 and 0 to 0.98, respectively. The species-specific microsatellites developed in this study can be used to inferred genetic diversity, breeding pattern and population genetic structure of G. sulphureus. In addition, the breeding pattern, genetic diversity and population genetic structure of G. sulphureus remain poorly understood. 2019-06 Thesis http://eprints.usm.my/48228/ http://eprints.usm.my/48228/1/Nur%20Aizatul%20Nathasha%20Binti%20Khizam%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
QH301 Biology |
| spellingShingle |
QH301 Biology Khizam, Nur Aizatul Nathasha Development Of Species-Specific Microsatellite Markers Of Termite Globitermes Sulphurues Haviland (Blattodea: Termitidae) For Genetic Population Analysis By Using Next-Generation Sequencing (Ngs) Approach |
| description |
Subterranean termite Globitermes sulphureus (higher termite) comprise a wide
distributed family of termites, found throughout the tropical regions of the world. Little
is known about G. sulphureus role in biology, dispersal range, and their control due to
lack of research to gather molecular data information. Hence, from the availability of
whole sequences of G. sulphureus, a great opportunity to develop species-specific
microsatellite markers can be done. In this study, a total of 243,057 transcripts were
generated from transcriptome dataset of G. sulphureus, with dinucleotide repeats being
the most abundant. From these, a total of new 30 species-specific microsatellite
markers were designed using next-generation sequencing (NGS) procedures. All of 30
markers were successfully amplified and showed visible scorable polymorphism. The
polymorphic information content (PIC) values showed greater than 0.5 in which
proved that all markers here had high level of polymorphism, with 3 to 9 alleles per
locus were identified. The observed and expected heterozygosities ranged from 0 to
0.83 and 0 to 0.98, respectively. The species-specific microsatellites developed in this
study can be used to inferred genetic diversity, breeding pattern and population genetic
structure of G. sulphureus. In addition, the breeding pattern, genetic diversity and
population genetic structure of G. sulphureus remain poorly understood. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Khizam, Nur Aizatul Nathasha |
| author_facet |
Khizam, Nur Aizatul Nathasha |
| author_sort |
Khizam, Nur Aizatul Nathasha |
| title |
Development Of Species-Specific
Microsatellite Markers Of Termite
Globitermes Sulphurues Haviland (Blattodea:
Termitidae) For Genetic Population
Analysis By Using Next-Generation
Sequencing (Ngs) Approach |
| title_short |
Development Of Species-Specific
Microsatellite Markers Of Termite
Globitermes Sulphurues Haviland (Blattodea:
Termitidae) For Genetic Population
Analysis By Using Next-Generation
Sequencing (Ngs) Approach |
| title_full |
Development Of Species-Specific
Microsatellite Markers Of Termite
Globitermes Sulphurues Haviland (Blattodea:
Termitidae) For Genetic Population
Analysis By Using Next-Generation
Sequencing (Ngs) Approach |
| title_fullStr |
Development Of Species-Specific
Microsatellite Markers Of Termite
Globitermes Sulphurues Haviland (Blattodea:
Termitidae) For Genetic Population
Analysis By Using Next-Generation
Sequencing (Ngs) Approach |
| title_full_unstemmed |
Development Of Species-Specific
Microsatellite Markers Of Termite
Globitermes Sulphurues Haviland (Blattodea:
Termitidae) For Genetic Population
Analysis By Using Next-Generation
Sequencing (Ngs) Approach |
| title_sort |
development of species-specific
microsatellite markers of termite
globitermes sulphurues haviland (blattodea:
termitidae) for genetic population
analysis by using next-generation
sequencing (ngs) approach |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Pusat Pengajian Sains Kajihayat |
| publishDate |
2019 |
| url |
http://eprints.usm.my/48228/1/Nur%20Aizatul%20Nathasha%20Binti%20Khizam%20cut.pdf |
| _version_ |
1747821905746853888 |