Investigating Insecticide Resistance Mechanisms In Dengue Vectors Population; Aedes Aegypti And Aedes Albopictus In Penang Island

In Malaysia, the vector of arboviral diseases especially Aedes aegypti and Ae. albopictus has long been known as a vector that could transmit dengue viruses. Currently, chemical control is considered as the most efficient way to control the dengue vectors in the dengue outbreak areas. However, the h...

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Main Author:	Jalaluddin, Nur Iwani
Format:	Thesis
Language:	English
Published:	2019
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/49313/1/Iwani%20MSc%20Corrected%20IPS%20cut.pdf
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id	my-usm-ep.49313
record_format	uketd_dc
spelling	my-usm-ep.493132021-06-17T13:47:09Z Investigating Insecticide Resistance Mechanisms In Dengue Vectors Population; Aedes Aegypti And Aedes Albopictus In Penang Island 2019-08 Jalaluddin, Nur Iwani QH1-278.5 Natural history (General) In Malaysia, the vector of arboviral diseases especially Aedes aegypti and Ae. albopictus has long been known as a vector that could transmit dengue viruses. Currently, chemical control is considered as the most efficient way to control the dengue vectors in the dengue outbreak areas. However, the heavy reliance on chemical control has resulted in the development of insecticide resistance in the dengue vectors due to the presence of insecticide resistance mechanism. This study highlights the susceptibility status of both dengue vectors exposed to permethrin and malathion insecticides via WHO adult mosquito bioassay test. The mechanisms involved in the insecticide resistance of dengue vectors by focusing on target-site resistance mechanism via genotyping the voltage gated sodium channel gene (VGSC), and Acetylcholinesterase gene (Ace-1) have been identified via polymerase-chain reaction (PCR) assays. The presence of metabolic resistance mechanisms has been investigated through biochemical enzyme assays and quantitative PCR (qPCR) in the samples of dengue vectors collected from Sungai Ara (SA), Jelutong (JG) and Balik Pulau (BP) areas of Penang Island. The insecticide susceptibility study showed that the adult Ae. aegypti samples from SA, JG and BP were resistant towards permethrin 0.25% and 0.75%, while the Ae. aegypti from all three sampling areas were susceptible towards malathion 5%. 2019-08 Thesis http://eprints.usm.my/49313/ http://eprints.usm.my/49313/1/Iwani%20MSc%20Corrected%20IPS%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Jalaluddin, Nur Iwani Investigating Insecticide Resistance Mechanisms In Dengue Vectors Population; Aedes Aegypti And Aedes Albopictus In Penang Island
description	In Malaysia, the vector of arboviral diseases especially Aedes aegypti and Ae. albopictus has long been known as a vector that could transmit dengue viruses. Currently, chemical control is considered as the most efficient way to control the dengue vectors in the dengue outbreak areas. However, the heavy reliance on chemical control has resulted in the development of insecticide resistance in the dengue vectors due to the presence of insecticide resistance mechanism. This study highlights the susceptibility status of both dengue vectors exposed to permethrin and malathion insecticides via WHO adult mosquito bioassay test. The mechanisms involved in the insecticide resistance of dengue vectors by focusing on target-site resistance mechanism via genotyping the voltage gated sodium channel gene (VGSC), and Acetylcholinesterase gene (Ace-1) have been identified via polymerase-chain reaction (PCR) assays. The presence of metabolic resistance mechanisms has been investigated through biochemical enzyme assays and quantitative PCR (qPCR) in the samples of dengue vectors collected from Sungai Ara (SA), Jelutong (JG) and Balik Pulau (BP) areas of Penang Island. The insecticide susceptibility study showed that the adult Ae. aegypti samples from SA, JG and BP were resistant towards permethrin 0.25% and 0.75%, while the Ae. aegypti from all three sampling areas were susceptible towards malathion 5%.
format	Thesis
qualification_level	Master's degree
author	Jalaluddin, Nur Iwani
author_facet	Jalaluddin, Nur Iwani
author_sort	Jalaluddin, Nur Iwani
title	Investigating Insecticide Resistance Mechanisms In Dengue Vectors Population; Aedes Aegypti And Aedes Albopictus In Penang Island
title_short	Investigating Insecticide Resistance Mechanisms In Dengue Vectors Population; Aedes Aegypti And Aedes Albopictus In Penang Island
title_full	Investigating Insecticide Resistance Mechanisms In Dengue Vectors Population; Aedes Aegypti And Aedes Albopictus In Penang Island
title_fullStr	Investigating Insecticide Resistance Mechanisms In Dengue Vectors Population; Aedes Aegypti And Aedes Albopictus In Penang Island
title_full_unstemmed	Investigating Insecticide Resistance Mechanisms In Dengue Vectors Population; Aedes Aegypti And Aedes Albopictus In Penang Island
title_sort	investigating insecticide resistance mechanisms in dengue vectors population; aedes aegypti and aedes albopictus in penang island
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2019
url	http://eprints.usm.my/49313/1/Iwani%20MSc%20Corrected%20IPS%20cut.pdf
_version_	1747821990289342464

Investigating Insecticide Resistance Mechanisms In Dengue Vectors Population; Aedes Aegypti And Aedes Albopictus In Penang Island

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