Feasibility Of Manganese Sequestration By Genetically Modified Escherichia coli K-12

Manganese, released by industries through wastewater, caused long term effect on human’s neurological functions. Application of chemical treatments will generate more waste sludge when cleaning the metal contaminant in diluted concentration. Thus, application of genetically modified Escherichia c...

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Main Author:	Awang, Huda
Format:	Thesis
Language:	English
Published:	2018
Subjects:	T1-995 Technology(General)
Online Access:	http://eprints.usm.my/49576/1/HUDA%20BINTI%20AWANG%20-%20FEASIBILITY%20OF%20MANGANESE%20SEQUESTRATION.pdf
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spelling	my-usm-ep.495762021-08-06T02:02:44Z Feasibility Of Manganese Sequestration By Genetically Modified Escherichia coli K-12 2018-08 Awang, Huda T1-995 Technology(General) Manganese, released by industries through wastewater, caused long term effect on human’s neurological functions. Application of chemical treatments will generate more waste sludge when cleaning the metal contaminant in diluted concentration. Thus, application of genetically modified Escherichia coli K-12 as a biosorbent was attempted to solve the problems. The objectives of this research were to study the feasibility of the techniques of preparing targeted DNA fragment, plasmid (pUC19) and host cells (E. coli K-12) for successful transformation, to develop a random genetic manipulation (genetically modified) for E. coli K-12 and to determine the effect of genetically modified E. coli K-12 in sequestering manganese. A DNA fragment had been isolated from Ceriporiopsis subvermispora FP105752, cloned into plasmid pUC19 and transformed into E. coli K-12. The transformed E. coli K-12 colonies were bigger in size with light orange colour. The modified DNAof E. coli K-12 was sequenced and analysed using Basic Local Alignment Search Tool (BLAST) to identify the cloned DNA fragment. The cloned DNA fragment was identified as Ascomycota sp. ARIZ RT Ash-1 internal transcribed spacer 1 with 82% of similarity indicating that random genetic manipulation towards E. coli K-12 was achieved. The genetically modified E. coli K-12 had been compared to the nongenetically modified E. coli K-12 by testing both strains in Luria Bertani media containing 200, 400, 600, 800 and 1 000 μM of MnSO4. The genetically modified E. coli K-12 was a more effective biosorbent compared to the non-genetically modified E. coli K-12 (p< 0.05). 2018-08 Thesis http://eprints.usm.my/49576/ http://eprints.usm.my/49576/1/HUDA%20BINTI%20AWANG%20-%20FEASIBILITY%20OF%20MANGANESE%20SEQUESTRATION.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Teknologi Industri
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	T1-995 Technology(General)
spellingShingle	T1-995 Technology(General) Awang, Huda Feasibility Of Manganese Sequestration By Genetically Modified Escherichia coli K-12
description	Manganese, released by industries through wastewater, caused long term effect on human’s neurological functions. Application of chemical treatments will generate more waste sludge when cleaning the metal contaminant in diluted concentration. Thus, application of genetically modified Escherichia coli K-12 as a biosorbent was attempted to solve the problems. The objectives of this research were to study the feasibility of the techniques of preparing targeted DNA fragment, plasmid (pUC19) and host cells (E. coli K-12) for successful transformation, to develop a random genetic manipulation (genetically modified) for E. coli K-12 and to determine the effect of genetically modified E. coli K-12 in sequestering manganese. A DNA fragment had been isolated from Ceriporiopsis subvermispora FP105752, cloned into plasmid pUC19 and transformed into E. coli K-12. The transformed E. coli K-12 colonies were bigger in size with light orange colour. The modified DNAof E. coli K-12 was sequenced and analysed using Basic Local Alignment Search Tool (BLAST) to identify the cloned DNA fragment. The cloned DNA fragment was identified as Ascomycota sp. ARIZ RT Ash-1 internal transcribed spacer 1 with 82% of similarity indicating that random genetic manipulation towards E. coli K-12 was achieved. The genetically modified E. coli K-12 had been compared to the nongenetically modified E. coli K-12 by testing both strains in Luria Bertani media containing 200, 400, 600, 800 and 1 000 μM of MnSO4. The genetically modified E. coli K-12 was a more effective biosorbent compared to the non-genetically modified E. coli K-12 (p< 0.05).
format	Thesis
qualification_level	Master's degree
author	Awang, Huda
author_facet	Awang, Huda
author_sort	Awang, Huda
title	Feasibility Of Manganese Sequestration By Genetically Modified Escherichia coli K-12
title_short	Feasibility Of Manganese Sequestration By Genetically Modified Escherichia coli K-12
title_full	Feasibility Of Manganese Sequestration By Genetically Modified Escherichia coli K-12
title_fullStr	Feasibility Of Manganese Sequestration By Genetically Modified Escherichia coli K-12
title_full_unstemmed	Feasibility Of Manganese Sequestration By Genetically Modified Escherichia coli K-12
title_sort	feasibility of manganese sequestration by genetically modified escherichia coli k-12
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Teknologi Industri
publishDate	2018
url	http://eprints.usm.my/49576/1/HUDA%20BINTI%20AWANG%20-%20FEASIBILITY%20OF%20MANGANESE%20SEQUESTRATION.pdf
_version_	1747822003785564160

Feasibility Of Manganese Sequestration By Genetically Modified Escherichia coli K-12

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