Ultrasonic Assisted Extraction Of Etlingera Elatior Leaves, Optimization, Characterization And Kinetic Studies
Etlingera elatior (E. elatior) is a herbaceous plant that is widely cultivated but the leaves are discarded as waste. Studies have shown that the leaves have potential in cosmetics formulation applications. In an extraction process, optimization and kinetic studies are important to develop a proc...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2020
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/50470/1/Ultrasonic%20Assisted%20Extraction%20Of%20Etlingera%20Elatior%20Leaves%2C%20Optimization%2C%20Characterization%20And%20Kinetic%20Studies.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Etlingera elatior (E. elatior) is a herbaceous plant that is widely cultivated but
the leaves are discarded as waste. Studies have shown that the leaves have potential in
cosmetics formulation applications. In an extraction process, optimization and kinetic
studies are important to develop a process that results in higher extraction yield and
efficiency. In this study, the leaves extract of E. elatior were obtained by using
ultrasonic-assisted extraction (UAE) technique. Methanol solvent was found to be
most effective for extraction, followed by ethanol, acetone, ethyl acetate and n-hexane.
The highest yield attained from UAE was 21.10 ± 0.29 % at an extraction time of 15
min, extraction temperature of 50 °C and liquid to solid (L/S) ratio of 35 mL/g at fixed
ultrasonic power of 150 W. The UAE process was further optimized using response
surface methodology by applying Box-Behnken design. The optimal conditions for the
extraction were obtained at extraction time of 16 min, extraction temperature 55 °C
and liquid to solid (L/S) ratio 38 mL/g and with methanol as solvent. Under these
optimal conditions, the experimental yield of leaves extract obtained was 21.42 %,
which matched well with the predicted model. Based on SEM images, ultrasonic
treatment induced fissures on the surface of E. elatior leaves. For kinetics studies,
Patricelli’s model fitted well to the extraction data with R2 > 0.99 and mean relative
percentage deviation value of < 10 %. Among all solvent extracts of E. elatior leaves,
methanolic extract exhibited the highest total phenolic content (228.35 ± 17.34 mg
GAE/g) and has the highest antioxidant activity (IC50 value 66.25 ± 7.77 μg/mL). A reasonable correlation was found between antioxidant capacity and the TPC
(R2= 0.75). All solvent extracts possess tyrosinase inhibition activity, but methanolic
extracts showed the strongest tyrosinase inhibition activity (IC50 value
263.91 ± 13.61 μg/ml). Only methanolic and ethanolic extracts showed antibacterial
activity towards B. subtilis (gram-positive bacteria), but none of the solvent extracts
exhibited any antibacterial activity against E. coli (gram-negative bacteria). The study
demonstrated that the properties of E. elatior leaves extract obtained by using UAE
method may have potential for cosmeceutical applications. |
|---|