Recombinant salmonella typhi ty21a as vaccine candidate against tuberculosis
A recombinant Salmonella Typhi Ty21 a vaccine was developed where the candidate [rSTvaciii(M2)] displaying a multi-epitopes mycobacterial antigen called the Vaciii(M2) (containing ubiquitin and 4 immunogenic mycobacterial epitopes of ESAT-6, PhoSI, Hsp 16.3, and MTB 8.4 genes) on the surface of S...
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my-usm-ep.515252022-02-15T01:38:06Z Recombinant salmonella typhi ty21a as vaccine candidate against tuberculosis 2008-10 Badrul Syam, Mat Zainuddin R Medicine (General) A recombinant Salmonella Typhi Ty21 a vaccine was developed where the candidate [rSTvaciii(M2)] displaying a multi-epitopes mycobacterial antigen called the Vaciii(M2) (containing ubiquitin and 4 immunogenic mycobacterial epitopes of ESAT-6, PhoSI, Hsp 16.3, and MTB 8.4 genes) on the surface of Salmonella Typhi Ty21a (Ty2la) using PgsA protein as a carrier. The expression of Vaciii(M2) protein on the surface of Ty21 a was verified by Western blot. A DNA vaccine was constructed namely as pVaxVaclll, encoding the Vaclll gene. Immunization in BALB/c mice with pVaxVaclll and rSTvaciii(M2) either as homologous or heterologous vaccination, induced strong Th 1-type responses based on high levels of in vitro IFN-y but low levels of IL-4 secretion. However, the heterologous primeboost vaccination using pVaxVaclll followed by rSTvaciii(M2) induced lower IFNy production compared to homologous rSTvaciii(M2) or pVaxVaclll vaccination. Since the protective immunity against tuberculosis required Th 1-type response, these rSTvaclii(M2) and pVaxVaclll have potential as effective vaccine candidates against tuberculosis. 2008-10 Thesis http://eprints.usm.my/51525/ http://eprints.usm.my/51525/1/BADRUL%20SYAM%20BIN%20MAT%20ZAINUDDIN%20-%2024%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan |
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English |
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R Medicine (General) |
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R Medicine (General) Badrul Syam, Mat Zainuddin Recombinant salmonella typhi ty21a as vaccine candidate against tuberculosis |
description |
A recombinant Salmonella Typhi Ty21 a vaccine was developed where the candidate
[rSTvaciii(M2)] displaying a multi-epitopes mycobacterial antigen called the
Vaciii(M2) (containing ubiquitin and 4 immunogenic mycobacterial epitopes of
ESAT-6, PhoSI, Hsp 16.3, and MTB 8.4 genes) on the surface of Salmonella Typhi
Ty21a (Ty2la) using PgsA protein as a carrier. The expression of Vaciii(M2)
protein on the surface of Ty21 a was verified by Western blot. A DNA vaccine was
constructed namely as pVaxVaclll, encoding the Vaclll gene. Immunization in
BALB/c mice with pVaxVaclll and rSTvaciii(M2) either as homologous or
heterologous vaccination, induced strong Th 1-type responses based on high levels of
in vitro IFN-y but low levels of IL-4 secretion. However, the heterologous primeboost
vaccination using pVaxVaclll followed by rSTvaciii(M2) induced lower IFNy
production compared to homologous rSTvaciii(M2) or pVaxVaclll vaccination.
Since the protective immunity against tuberculosis required Th 1-type response, these
rSTvaclii(M2) and pVaxVaclll have potential as effective vaccine candidates
against tuberculosis. |
format |
Thesis |
qualification_level |
Master's degree |
author |
Badrul Syam, Mat Zainuddin |
author_facet |
Badrul Syam, Mat Zainuddin |
author_sort |
Badrul Syam, Mat Zainuddin |
title |
Recombinant salmonella typhi ty21a as
vaccine candidate against
tuberculosis |
title_short |
Recombinant salmonella typhi ty21a as
vaccine candidate against
tuberculosis |
title_full |
Recombinant salmonella typhi ty21a as
vaccine candidate against
tuberculosis |
title_fullStr |
Recombinant salmonella typhi ty21a as
vaccine candidate against
tuberculosis |
title_full_unstemmed |
Recombinant salmonella typhi ty21a as
vaccine candidate against
tuberculosis |
title_sort |
recombinant salmonella typhi ty21a as
vaccine candidate against
tuberculosis |
granting_institution |
Universiti Sains Malaysia |
granting_department |
Pusat Pengajian Sains Perubatan |
publishDate |
2008 |
url |
http://eprints.usm.my/51525/1/BADRUL%20SYAM%20BIN%20MAT%20ZAINUDDIN%20-%2024%20pages.pdf |
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1747822076063907840 |