Micropropagation For Ficus Carica L. Cv. Panachee Using Light-Emitting Diodes System

Ficus carica L. is challenging and time-consuming through conventional plant propagation methods due to the low survival rate. This study aims to establish an optimised protocol using response surface methodology (RSM) for micropropagation and meristem culture on F. carica L. cv. Panachee under ligh...

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Main Author:	Eyu, Chan Hong
Format:	Thesis
Language:	English
Published:	2021
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/51910/1/EYU%20CHAN%20HONG.pdf
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id	my-usm-ep.51910
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spelling	my-usm-ep.519102022-03-14T00:52:46Z Micropropagation For Ficus Carica L. Cv. Panachee Using Light-Emitting Diodes System 2021-02 Eyu, Chan Hong QH1-278.5 Natural history (General) Ficus carica L. is challenging and time-consuming through conventional plant propagation methods due to the low survival rate. This study aims to establish an optimised protocol using response surface methodology (RSM) for micropropagation and meristem culture on F. carica L. cv. Panachee under light-emitting diodes (LEDs) application using different spectra. In 3-significant factors by Box-Behken design (BBD) experiment, meristem culture was optimised by incubating meristematic tissue (0.5 – 1.0 mm) onto filter paper bridge partially soaked in liquid Murashige and Skoog (MS) medium supplemented with 50 μM zeatin (X1) under red LEDs at the intensity of 1.26 μ mol/s (X3) (Ymeristemred = 0.882 + 0.271X1 - 0.250X3red – 0.558X1X3*red). For micropropagation, 4-significant factors by BBD experiment were used to developed second-order mathematical models, which resulted in incomparable growth and development at different LED spectra. Optimised micropropagation protocols with every four-weeks interval for each stage can be integrated with a SMART LED system with the incubation on gelled MS added with 30 μM IAA (B) and 1.0 g/L activated charcoal (C) under the combination of red, green, and blue (RGB) LEDs at 22.20 μmol/s (DRGB) that resulted 1.88 shoots (Number of shoots RGB LED = 0.4148 + 0.1667B + 0.3000C + 0.2500C + 0.400BC + 0.350CDRGB). Subsequently, followed by optimisation through gelled MS fortified with 40 μM BAP (A), 30 μM IAA (B), and 1.0 g/L activated charcoal (C) under red LEDs to achieve the greatest height increment at 32.97 mm after four weeks (Final height red LED = 9.078 + 1.767A + 1.733B + 1.822C + 2.73A2 + 4.68B2 + 2.55AB + 2.95AC + 5.65BC). 2021-02 Thesis http://eprints.usm.my/51910/ http://eprints.usm.my/51910/1/EYU%20CHAN%20HONG.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Eyu, Chan Hong Micropropagation For Ficus Carica L. Cv. Panachee Using Light-Emitting Diodes System
description	Ficus carica L. is challenging and time-consuming through conventional plant propagation methods due to the low survival rate. This study aims to establish an optimised protocol using response surface methodology (RSM) for micropropagation and meristem culture on F. carica L. cv. Panachee under light-emitting diodes (LEDs) application using different spectra. In 3-significant factors by Box-Behken design (BBD) experiment, meristem culture was optimised by incubating meristematic tissue (0.5 – 1.0 mm) onto filter paper bridge partially soaked in liquid Murashige and Skoog (MS) medium supplemented with 50 μM zeatin (X1) under red LEDs at the intensity of 1.26 μ mol/s (X3) (Ymeristemred = 0.882 + 0.271X1 - 0.250X3red – 0.558X1X3*red). For micropropagation, 4-significant factors by BBD experiment were used to developed second-order mathematical models, which resulted in incomparable growth and development at different LED spectra. Optimised micropropagation protocols with every four-weeks interval for each stage can be integrated with a SMART LED system with the incubation on gelled MS added with 30 μM IAA (B) and 1.0 g/L activated charcoal (C) under the combination of red, green, and blue (RGB) LEDs at 22.20 μmol/s (DRGB) that resulted 1.88 shoots (Number of shoots RGB LED = 0.4148 + 0.1667B + 0.3000C + 0.2500C + 0.400BC + 0.350CDRGB). Subsequently, followed by optimisation through gelled MS fortified with 40 μM BAP (A), 30 μM IAA (B), and 1.0 g/L activated charcoal (C) under red LEDs to achieve the greatest height increment at 32.97 mm after four weeks (Final height red LED = 9.078 + 1.767A + 1.733B + 1.822C + 2.73A2 + 4.68B2 + 2.55AB + 2.95AC + 5.65BC).
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Eyu, Chan Hong
author_facet	Eyu, Chan Hong
author_sort	Eyu, Chan Hong
title	Micropropagation For Ficus Carica L. Cv. Panachee Using Light-Emitting Diodes System
title_short	Micropropagation For Ficus Carica L. Cv. Panachee Using Light-Emitting Diodes System
title_full	Micropropagation For Ficus Carica L. Cv. Panachee Using Light-Emitting Diodes System
title_fullStr	Micropropagation For Ficus Carica L. Cv. Panachee Using Light-Emitting Diodes System
title_full_unstemmed	Micropropagation For Ficus Carica L. Cv. Panachee Using Light-Emitting Diodes System
title_sort	micropropagation for ficus carica l. cv. panachee using light-emitting diodes system
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2021
url	http://eprints.usm.my/51910/1/EYU%20CHAN%20HONG.pdf
_version_	1747822111751143424

Micropropagation For Ficus Carica L. Cv. Panachee Using Light-Emitting Diodes System

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