The Antiproliferative Effects Of Moringa Oleifera Leaf Extracts And Silver Nanoparticles On Human Leukemia Kasumi-1 Cells

Moringa oleifera, also commonly known as ‘moringa’, is widely cultivated in tropical and subtropical regions across the globe. All parts of the moringa tree can be utilised and extensive studies have shown that various parts of the moringa tree exhibit anti-cancer properties. This study determined t...

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Main Author:	Khor, Kang Zi
Format:	Thesis
Language:	English
Published:	2020
Subjects:	RK1-715 Dentistry
Online Access:	http://eprints.usm.my/52170/1/KHOR%20KANG%20ZI%20-%20TESIS%20cut.pdf
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id	my-usm-ep.52170
record_format	uketd_dc
spelling	my-usm-ep.521702022-04-04T08:44:38Z The Antiproliferative Effects Of Moringa Oleifera Leaf Extracts And Silver Nanoparticles On Human Leukemia Kasumi-1 Cells 2020-04 Khor, Kang Zi RK1-715 Dentistry Moringa oleifera, also commonly known as ‘moringa’, is widely cultivated in tropical and subtropical regions across the globe. All parts of the moringa tree can be utilised and extensive studies have shown that various parts of the moringa tree exhibit anti-cancer properties. This study determined the effects of sequential moringa leaf extracts and silver nanoparticles synthesised from moringa leaf extract on Kasumi-1 leukemia cells. Dried moringa leaf powder was sequentially extracted with the assistance of ultrasound starting with absolute ethanol, followed by 50% ethanol, and finally, deionised water. The aqueous extract was incorporated to synthesise silver nanoparticles, whereby the optimum conditions to generate moringa silver nanoparticles (MO-AgNPs) were eight hours of incubation at 60 °C with 1 mM silver nitrate and 1% moringa aqueous extract from sequential extraction. The three various extracts and MO-AgNPs were used to treat Kasumi-1 cells for 24, 48, and 72 hours with concentrations ranging between 400 and 12.5 μg/mL, while cell viability was determined with 3(4, 5-dimethythiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The experimental outcomes revealed that after 72 hours of treatment, the moringa leaf ethanolic extract displayed the strongest inhibitory effects on Kasumi-1 cells with IC50 of 10 μg/mL, in comparison to moringa leaf 50% ethanolic extract (25 μg/mL) and aqueous extract (>400 μg/mL). Interestingly MO-AgNPs exhibited the strongest cytotoxic effects on Kasumi-1 cells with an IC50 of 7.5 μg/mL. 2020-04 Thesis http://eprints.usm.my/52170/ http://eprints.usm.my/52170/1/KHOR%20KANG%20ZI%20-%20TESIS%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RK1-715 Dentistry
spellingShingle	RK1-715 Dentistry Khor, Kang Zi The Antiproliferative Effects Of Moringa Oleifera Leaf Extracts And Silver Nanoparticles On Human Leukemia Kasumi-1 Cells
description	Moringa oleifera, also commonly known as ‘moringa’, is widely cultivated in tropical and subtropical regions across the globe. All parts of the moringa tree can be utilised and extensive studies have shown that various parts of the moringa tree exhibit anti-cancer properties. This study determined the effects of sequential moringa leaf extracts and silver nanoparticles synthesised from moringa leaf extract on Kasumi-1 leukemia cells. Dried moringa leaf powder was sequentially extracted with the assistance of ultrasound starting with absolute ethanol, followed by 50% ethanol, and finally, deionised water. The aqueous extract was incorporated to synthesise silver nanoparticles, whereby the optimum conditions to generate moringa silver nanoparticles (MO-AgNPs) were eight hours of incubation at 60 °C with 1 mM silver nitrate and 1% moringa aqueous extract from sequential extraction. The three various extracts and MO-AgNPs were used to treat Kasumi-1 cells for 24, 48, and 72 hours with concentrations ranging between 400 and 12.5 μg/mL, while cell viability was determined with 3(4, 5-dimethythiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The experimental outcomes revealed that after 72 hours of treatment, the moringa leaf ethanolic extract displayed the strongest inhibitory effects on Kasumi-1 cells with IC50 of 10 μg/mL, in comparison to moringa leaf 50% ethanolic extract (25 μg/mL) and aqueous extract (>400 μg/mL). Interestingly MO-AgNPs exhibited the strongest cytotoxic effects on Kasumi-1 cells with an IC50 of 7.5 μg/mL.
format	Thesis
qualification_level	Master's degree
author	Khor, Kang Zi
author_facet	Khor, Kang Zi
author_sort	Khor, Kang Zi
title	The Antiproliferative Effects Of Moringa Oleifera Leaf Extracts And Silver Nanoparticles On Human Leukemia Kasumi-1 Cells
title_short	The Antiproliferative Effects Of Moringa Oleifera Leaf Extracts And Silver Nanoparticles On Human Leukemia Kasumi-1 Cells
title_full	The Antiproliferative Effects Of Moringa Oleifera Leaf Extracts And Silver Nanoparticles On Human Leukemia Kasumi-1 Cells
title_fullStr	The Antiproliferative Effects Of Moringa Oleifera Leaf Extracts And Silver Nanoparticles On Human Leukemia Kasumi-1 Cells
title_full_unstemmed	The Antiproliferative Effects Of Moringa Oleifera Leaf Extracts And Silver Nanoparticles On Human Leukemia Kasumi-1 Cells
title_sort	antiproliferative effects of moringa oleifera leaf extracts and silver nanoparticles on human leukemia kasumi-1 cells
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2020
url	http://eprints.usm.my/52170/1/KHOR%20KANG%20ZI%20-%20TESIS%20cut.pdf
_version_	1747822139279409152

The Antiproliferative Effects Of Moringa Oleifera Leaf Extracts And Silver Nanoparticles On Human Leukemia Kasumi-1 Cells

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