Paretage Assigment Gene Expressio Of Growth Related Gene In Snakehead Murrel, Channa Striata

Haruan (Channa striata) is a fresh water fish known for its medicinal value and is an important source of protein in Southeast Asia. The demand for this fish is still high; hence this species has good market potential for aquaculture industry in Malaysia. Furthermore, snakehead farming is not pop...

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Main Author: Fong, Pooi Har
Format: Thesis
Language:English
Published: 2020
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Online Access:http://eprints.usm.my/53994/1/Fong.pdf
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spelling my-usm-ep.539942022-08-11T02:07:46Z Paretage Assigment Gene Expressio Of Growth Related Gene In Snakehead Murrel, Channa Striata 2020-03 Fong, Pooi Har QH1 Natural history (General - Including nature conservation, geographical distribution) Haruan (Channa striata) is a fresh water fish known for its medicinal value and is an important source of protein in Southeast Asia. The demand for this fish is still high; hence this species has good market potential for aquaculture industry in Malaysia. Furthermore, snakehead farming is not popular and there is yet a selective breeding as well as systematic hatchery programmes developed in Malaysia. Hence, the first objective of this study was to investigate the feasibility of using the existing microsatellite markers in genotyping C. striata for parentage identification and pedigree management. The next objective is to establish a reference for transcriptome library for gene expression study. With the transcriptome library, differentially expressed genes in response of fast and slow growth could be identified and applied in snakehead murrel selective breeding programmes. Accurate parentage identification for pedigree management is integral to a successful systematic hatchery programme especially in aquaculture industry to prevent inbreeding depression. Hence, the feasibility of seven pairs of microsatellite markers characterized previously for a population genetics study of C. striata was evaluated in this study. A total of 211 offspring from 22 brood stocks kept in six separated tanks were genotyped according to tanks and were later assigned back to these 22 brood stocks using five different parentage assignment software. Among these software, Colony was the best software to successfully allocate the offspring back to their parents. In addition, the results of the parentage allocations revealed that mating behaviour of this species is polygamous and polyandry. 2020-03 Thesis http://eprints.usm.my/53994/ http://eprints.usm.my/53994/1/Fong.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QH1 Natural history (General - Including nature conservation
geographical distribution)
spellingShingle QH1 Natural history (General - Including nature conservation
geographical distribution)
Fong, Pooi Har
Paretage Assigment Gene Expressio Of Growth Related Gene In Snakehead Murrel, Channa Striata
description Haruan (Channa striata) is a fresh water fish known for its medicinal value and is an important source of protein in Southeast Asia. The demand for this fish is still high; hence this species has good market potential for aquaculture industry in Malaysia. Furthermore, snakehead farming is not popular and there is yet a selective breeding as well as systematic hatchery programmes developed in Malaysia. Hence, the first objective of this study was to investigate the feasibility of using the existing microsatellite markers in genotyping C. striata for parentage identification and pedigree management. The next objective is to establish a reference for transcriptome library for gene expression study. With the transcriptome library, differentially expressed genes in response of fast and slow growth could be identified and applied in snakehead murrel selective breeding programmes. Accurate parentage identification for pedigree management is integral to a successful systematic hatchery programme especially in aquaculture industry to prevent inbreeding depression. Hence, the feasibility of seven pairs of microsatellite markers characterized previously for a population genetics study of C. striata was evaluated in this study. A total of 211 offspring from 22 brood stocks kept in six separated tanks were genotyped according to tanks and were later assigned back to these 22 brood stocks using five different parentage assignment software. Among these software, Colony was the best software to successfully allocate the offspring back to their parents. In addition, the results of the parentage allocations revealed that mating behaviour of this species is polygamous and polyandry.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Fong, Pooi Har
author_facet Fong, Pooi Har
author_sort Fong, Pooi Har
title Paretage Assigment Gene Expressio Of Growth Related Gene In Snakehead Murrel, Channa Striata
title_short Paretage Assigment Gene Expressio Of Growth Related Gene In Snakehead Murrel, Channa Striata
title_full Paretage Assigment Gene Expressio Of Growth Related Gene In Snakehead Murrel, Channa Striata
title_fullStr Paretage Assigment Gene Expressio Of Growth Related Gene In Snakehead Murrel, Channa Striata
title_full_unstemmed Paretage Assigment Gene Expressio Of Growth Related Gene In Snakehead Murrel, Channa Striata
title_sort paretage assigment gene expressio of growth related gene in snakehead murrel, channa striata
granting_institution Perpustakaan Hamzah Sendut
granting_department Pusat Pengajian Sains Kajihayat
publishDate 2020
url http://eprints.usm.my/53994/1/Fong.pdf
_version_ 1747822268899131392