Paretage Assigment Gene Expressio Of Growth Related Gene In Snakehead Murrel, Channa Striata
Haruan (Channa striata) is a fresh water fish known for its medicinal value and is an important source of protein in Southeast Asia. The demand for this fish is still high; hence this species has good market potential for aquaculture industry in Malaysia. Furthermore, snakehead farming is not pop...
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my-usm-ep.539942022-08-11T02:07:46Z Paretage Assigment Gene Expressio Of Growth Related Gene In Snakehead Murrel, Channa Striata 2020-03 Fong, Pooi Har QH1 Natural history (General - Including nature conservation, geographical distribution) Haruan (Channa striata) is a fresh water fish known for its medicinal value and is an important source of protein in Southeast Asia. The demand for this fish is still high; hence this species has good market potential for aquaculture industry in Malaysia. Furthermore, snakehead farming is not popular and there is yet a selective breeding as well as systematic hatchery programmes developed in Malaysia. Hence, the first objective of this study was to investigate the feasibility of using the existing microsatellite markers in genotyping C. striata for parentage identification and pedigree management. The next objective is to establish a reference for transcriptome library for gene expression study. With the transcriptome library, differentially expressed genes in response of fast and slow growth could be identified and applied in snakehead murrel selective breeding programmes. Accurate parentage identification for pedigree management is integral to a successful systematic hatchery programme especially in aquaculture industry to prevent inbreeding depression. Hence, the feasibility of seven pairs of microsatellite markers characterized previously for a population genetics study of C. striata was evaluated in this study. A total of 211 offspring from 22 brood stocks kept in six separated tanks were genotyped according to tanks and were later assigned back to these 22 brood stocks using five different parentage assignment software. Among these software, Colony was the best software to successfully allocate the offspring back to their parents. In addition, the results of the parentage allocations revealed that mating behaviour of this species is polygamous and polyandry. 2020-03 Thesis http://eprints.usm.my/53994/ http://eprints.usm.my/53994/1/Fong.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat |
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QH1 Natural history (General - Including nature conservation geographical distribution) |
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QH1 Natural history (General - Including nature conservation geographical distribution) Fong, Pooi Har Paretage Assigment Gene Expressio Of Growth Related Gene In Snakehead Murrel, Channa Striata |
description |
Haruan (Channa striata) is a fresh water fish known for its medicinal value
and is an important source of protein in Southeast Asia. The demand for this fish is
still high; hence this species has good market potential for aquaculture industry in
Malaysia. Furthermore, snakehead farming is not popular and there is yet a selective
breeding as well as systematic hatchery programmes developed in Malaysia. Hence,
the first objective of this study was to investigate the feasibility of using the existing
microsatellite markers in genotyping C. striata for parentage identification and
pedigree management. The next objective is to establish a reference for
transcriptome library for gene expression study. With the transcriptome library,
differentially expressed genes in response of fast and slow growth could be identified
and applied in snakehead murrel selective breeding programmes. Accurate parentage
identification for pedigree management is integral to a successful systematic
hatchery programme especially in aquaculture industry to prevent inbreeding
depression. Hence, the feasibility of seven pairs of microsatellite markers
characterized previously for a population genetics study of C. striata was evaluated
in this study. A total of 211 offspring from 22 brood stocks kept in six separated
tanks were genotyped according to tanks and were later assigned back to these 22
brood stocks using five different parentage assignment software. Among these
software, Colony was the best software to successfully allocate the offspring back to
their parents. In addition, the results of the parentage allocations revealed that mating
behaviour of this species is polygamous and polyandry. |
format |
Thesis |
qualification_name |
Doctor of Philosophy (PhD.) |
qualification_level |
Doctorate |
author |
Fong, Pooi Har |
author_facet |
Fong, Pooi Har |
author_sort |
Fong, Pooi Har |
title |
Paretage Assigment Gene
Expressio Of Growth Related Gene In
Snakehead Murrel, Channa Striata |
title_short |
Paretage Assigment Gene
Expressio Of Growth Related Gene In
Snakehead Murrel, Channa Striata |
title_full |
Paretage Assigment Gene
Expressio Of Growth Related Gene In
Snakehead Murrel, Channa Striata |
title_fullStr |
Paretage Assigment Gene
Expressio Of Growth Related Gene In
Snakehead Murrel, Channa Striata |
title_full_unstemmed |
Paretage Assigment Gene
Expressio Of Growth Related Gene In
Snakehead Murrel, Channa Striata |
title_sort |
paretage assigment gene
expressio of growth related gene in
snakehead murrel, channa striata |
granting_institution |
Perpustakaan Hamzah Sendut |
granting_department |
Pusat Pengajian Sains Kajihayat |
publishDate |
2020 |
url |
http://eprints.usm.my/53994/1/Fong.pdf |
_version_ |
1747822268899131392 |