Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves

Common figs (Ficus carica) are highly valued fruit crops owing to its elevated nutrient content and vast economic and medicinal importance. Although native to the Middle East and Mediterranean region, the adaptability of figs has attributed to its cultivation also in tropical countries. However, cur...

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Main Author:	Tan, Li Vern
Format:	Thesis
Language:	English
Published:	2021
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/55059/1/TAN%20LI%20VERN%20-%20TESIS24.pdf
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id	my-usm-ep.55059
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spelling	my-usm-ep.550592022-09-30T11:55:17Z Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves 2021-10 Tan, Li Vern QH1 Natural history (General - Including nature conservation, geographical distribution) Common figs (Ficus carica) are highly valued fruit crops owing to its elevated nutrient content and vast economic and medicinal importance. Although native to the Middle East and Mediterranean region, the adaptability of figs has attributed to its cultivation also in tropical countries. However, current conventional cultivation methods applied for the propagation of plant stocks are restricted due to the difficulty in obtaining a large number of well-rooted, disease-free plantlets. Plant tissue culture particularly micropropagation has been successfully applied to propagate plant stocks for many commercial crops. The current study aims to establish an efficient protocol for the micropropagation of Ficus carica cv. Texas Everbearing (TE) and Lisa and to evaluate the anti-hyperglycemic potential of fig leaves. From the results obtained, explant surface sterilization was successfully performed with a maximum survival rate of 50%. The optimal shoot induction condition for the cultivar TE was nodal segments cultured horizontally in MS medium supplemented with 1.0 g/L AC, 10 g/L sucrose, 3.0 mg/L TDZ and 0.75 mg/L NAA, recording maximum shoot induction rate (100%), shoot number of 2.15 ± 0.10 shoots and shoot length of 1.05 ± 0.12 cm. For the cultivar Lisa, shoot induction rate of 100%, shoot number of 1.92 ± 0.14 shoots and shoot length of 2.11 ± 0.18 cm was obtained similarly by culturing nodal segments horizontally in MS medium supplemented with 1.0 g/L AC but with 20 g/L fructose, 2.0 mg/L ZEA and 1.0 mg/L NAA. Explants of both cultivars were then successfully rooted in WPM and acclimatized in a potting mix comprising of black soil, red soil and coco peat at a 3:1:1 ratio. In the evaluation of anti-hyperglycemic potential of fig leaves from F. carica cv. TE and Lisa on diabetic induced rats, leaf extracts were obtained via maceration in 50% ethanol for 72 hours. Leaf extract from fig cultivar TE and Lisa at 250 mg/kg showed a significant effect (p < 0.05) in the reduction fasting blood glucose level at day 14 (16.3 ± 2.1 and 17.6 ± 3.1 mmol/L respectively) and average water intake in week 2 (27.2 ± 2.4 and 27.5 ± 2.0 ml/100 g b.w. respectively) when compared to vehicle treated diabetic control, all reflecting a reduction in hyperglycemia. No significant difference was recorded on the body and organ weights when compared to diabetic control rats. This study has successfully documented an efficient micropropagation protocol for fig (Ficus carica) cultivars ‘Texas Everbearing’ and ‘Lisa’ for the production of plant stocks suitable for commercial field establishments and also highlights the potential of fig leaf extract from both fig cultivars in controlling diabetes. 2021-10 Thesis http://eprints.usm.my/55059/ http://eprints.usm.my/55059/1/TAN%20LI%20VERN%20-%20TESIS24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia. Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Tan, Li Vern Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves
description	Common figs (Ficus carica) are highly valued fruit crops owing to its elevated nutrient content and vast economic and medicinal importance. Although native to the Middle East and Mediterranean region, the adaptability of figs has attributed to its cultivation also in tropical countries. However, current conventional cultivation methods applied for the propagation of plant stocks are restricted due to the difficulty in obtaining a large number of well-rooted, disease-free plantlets. Plant tissue culture particularly micropropagation has been successfully applied to propagate plant stocks for many commercial crops. The current study aims to establish an efficient protocol for the micropropagation of Ficus carica cv. Texas Everbearing (TE) and Lisa and to evaluate the anti-hyperglycemic potential of fig leaves. From the results obtained, explant surface sterilization was successfully performed with a maximum survival rate of 50%. The optimal shoot induction condition for the cultivar TE was nodal segments cultured horizontally in MS medium supplemented with 1.0 g/L AC, 10 g/L sucrose, 3.0 mg/L TDZ and 0.75 mg/L NAA, recording maximum shoot induction rate (100%), shoot number of 2.15 ± 0.10 shoots and shoot length of 1.05 ± 0.12 cm. For the cultivar Lisa, shoot induction rate of 100%, shoot number of 1.92 ± 0.14 shoots and shoot length of 2.11 ± 0.18 cm was obtained similarly by culturing nodal segments horizontally in MS medium supplemented with 1.0 g/L AC but with 20 g/L fructose, 2.0 mg/L ZEA and 1.0 mg/L NAA. Explants of both cultivars were then successfully rooted in WPM and acclimatized in a potting mix comprising of black soil, red soil and coco peat at a 3:1:1 ratio. In the evaluation of anti-hyperglycemic potential of fig leaves from F. carica cv. TE and Lisa on diabetic induced rats, leaf extracts were obtained via maceration in 50% ethanol for 72 hours. Leaf extract from fig cultivar TE and Lisa at 250 mg/kg showed a significant effect (p < 0.05) in the reduction fasting blood glucose level at day 14 (16.3 ± 2.1 and 17.6 ± 3.1 mmol/L respectively) and average water intake in week 2 (27.2 ± 2.4 and 27.5 ± 2.0 ml/100 g b.w. respectively) when compared to vehicle treated diabetic control, all reflecting a reduction in hyperglycemia. No significant difference was recorded on the body and organ weights when compared to diabetic control rats. This study has successfully documented an efficient micropropagation protocol for fig (Ficus carica) cultivars ‘Texas Everbearing’ and ‘Lisa’ for the production of plant stocks suitable for commercial field establishments and also highlights the potential of fig leaf extract from both fig cultivars in controlling diabetes.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Tan, Li Vern
author_facet	Tan, Li Vern
author_sort	Tan, Li Vern
title	Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves
title_short	Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves
title_full	Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves
title_fullStr	Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves
title_full_unstemmed	Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves
title_sort	micropropagation of fig (ficus carica) cultivars ‘texas everbearing’ and ‘lisa’ & the anti-hyperglycemic potential of fig leaves
granting_institution	Universiti Sains Malaysia.
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2021
url	http://eprints.usm.my/55059/1/TAN%20LI%20VERN%20-%20TESIS24.pdf
_version_	1747822307131260928

Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves

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