Development Of Poly(Lactic Acid) Microspheres/Gelatin Coated Beta-Tricalcium Phosphate Scaffold For Bone Tissue Engineering Application

Three-dimensional interconnected porous structure and ideal mechanical strength are essential for biological scaffold in bone tissue engineering application. The main aim of this study is to develop multifunctional scaffold for bone tissue engineering application. In this research, gel casting met...

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Main Author:	Cheng, Shu Yen
Format:	Thesis
Language:	English
Published:	2019
Subjects:	T Technology
Online Access:	http://eprints.usm.my/55136/1/Development%20Of%20Poly%28Lactic%20Acid%29%20MicrospheresGelatin%20Coated%20Beta-Tricalcium%20Phosphate%20Scaffold%20For%20Bone%20Tissue%20Engineering%20Application_Cheng%20Shu%20Yen_N4_2019_ESAR.pdf
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spelling	my-usm-ep.551362022-10-05T01:53:51Z Development Of Poly(Lactic Acid) Microspheres/Gelatin Coated Beta-Tricalcium Phosphate Scaffold For Bone Tissue Engineering Application 2019-04-01 Cheng, Shu Yen T Technology TN Mining Engineering. Metallurgy Three-dimensional interconnected porous structure and ideal mechanical strength are essential for biological scaffold in bone tissue engineering application. The main aim of this study is to develop multifunctional scaffold for bone tissue engineering application. In this research, gel casting method was used to produce porous beta-tricalcium phosphate (β-TCP) scaffold. β-TCP scaffold coated with gelatin crosslinked with 1% glutaraldehyde (GA) showed optimum compressive strength where it improved around 139% compressive strength compared to uncoated scaffold. Uncoated β-TCP scaffold and the β-TCP scaffold coated with crosslinked gelatin showed their bioactive properties by taking 2 weeks to form apatite. The gelatin coating had slightly reduced protein-adsorption compared to pure β-TCP. In the second part, emulsion solvent evaporation was used to fabricate doxycycline loaded poly(lactic acid) (PLA) microspheres. Results showed that the 4% NaCl in external aqueous phase was the most efficient way to increase the encapsulation efficiency where 66% of the doxycycline was encapsulated and showed the largest inhibition growth zone diameter of E. coli at 16.15mm. Lastly, β-TCP scaffold coated with PLA microspheres had enhanced the compressive strength. The encapsulated doxycycline was released in a slower and sustained manner compared to free PLA microspheres. In short, PLA microspheres coated scaffold developed in the study shows multifunctional as it provides structural support, bioactivity and controlled drug delivery function for bone tissue engineering. 2019-04 Thesis http://eprints.usm.my/55136/ http://eprints.usm.my/55136/1/Development%20Of%20Poly%28Lactic%20Acid%29%20MicrospheresGelatin%20Coated%20Beta-Tricalcium%20Phosphate%20Scaffold%20For%20Bone%20Tissue%20Engineering%20Application_Cheng%20Shu%20Yen_N4_2019_ESAR.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Kejuruteraan Bahan dan Sumber Mineral
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	T Technology T Technology
spellingShingle	T Technology T Technology Cheng, Shu Yen Development Of Poly(Lactic Acid) Microspheres/Gelatin Coated Beta-Tricalcium Phosphate Scaffold For Bone Tissue Engineering Application
description	Three-dimensional interconnected porous structure and ideal mechanical strength are essential for biological scaffold in bone tissue engineering application. The main aim of this study is to develop multifunctional scaffold for bone tissue engineering application. In this research, gel casting method was used to produce porous beta-tricalcium phosphate (β-TCP) scaffold. β-TCP scaffold coated with gelatin crosslinked with 1% glutaraldehyde (GA) showed optimum compressive strength where it improved around 139% compressive strength compared to uncoated scaffold. Uncoated β-TCP scaffold and the β-TCP scaffold coated with crosslinked gelatin showed their bioactive properties by taking 2 weeks to form apatite. The gelatin coating had slightly reduced protein-adsorption compared to pure β-TCP. In the second part, emulsion solvent evaporation was used to fabricate doxycycline loaded poly(lactic acid) (PLA) microspheres. Results showed that the 4% NaCl in external aqueous phase was the most efficient way to increase the encapsulation efficiency where 66% of the doxycycline was encapsulated and showed the largest inhibition growth zone diameter of E. coli at 16.15mm. Lastly, β-TCP scaffold coated with PLA microspheres had enhanced the compressive strength. The encapsulated doxycycline was released in a slower and sustained manner compared to free PLA microspheres. In short, PLA microspheres coated scaffold developed in the study shows multifunctional as it provides structural support, bioactivity and controlled drug delivery function for bone tissue engineering.
format	Thesis
qualification_level	Master's degree
author	Cheng, Shu Yen
author_facet	Cheng, Shu Yen
author_sort	Cheng, Shu Yen
title	Development Of Poly(Lactic Acid) Microspheres/Gelatin Coated Beta-Tricalcium Phosphate Scaffold For Bone Tissue Engineering Application
title_short	Development Of Poly(Lactic Acid) Microspheres/Gelatin Coated Beta-Tricalcium Phosphate Scaffold For Bone Tissue Engineering Application
title_full	Development Of Poly(Lactic Acid) Microspheres/Gelatin Coated Beta-Tricalcium Phosphate Scaffold For Bone Tissue Engineering Application
title_fullStr	Development Of Poly(Lactic Acid) Microspheres/Gelatin Coated Beta-Tricalcium Phosphate Scaffold For Bone Tissue Engineering Application
title_full_unstemmed	Development Of Poly(Lactic Acid) Microspheres/Gelatin Coated Beta-Tricalcium Phosphate Scaffold For Bone Tissue Engineering Application
title_sort	development of poly(lactic acid) microspheres/gelatin coated beta-tricalcium phosphate scaffold for bone tissue engineering application
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Kejuruteraan Bahan dan Sumber Mineral
publishDate	2019
url	http://eprints.usm.my/55136/1/Development%20Of%20Poly%28Lactic%20Acid%29%20MicrospheresGelatin%20Coated%20Beta-Tricalcium%20Phosphate%20Scaffold%20For%20Bone%20Tissue%20Engineering%20Application_Cheng%20Shu%20Yen_N4_2019_ESAR.pdf
_version_	1747822314492264448

Development Of Poly(Lactic Acid) Microspheres/Gelatin Coated Beta-Tricalcium Phosphate Scaffold For Bone Tissue Engineering Application

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