A study on trichosporon isolation, its molecular identification, clinical manifestations and risk factors

Trichosporon infection is a rare opportunistic, life threatening mycosis in patients with predisposing factors such as underlying immunodeficiency. Conventional methods to diagnose trichosporonosis are inadequate compared to molecular methods in terms of species identification. We reviewed 155 pa...

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Main Author:	Azim, Wan Amani Wan Abdul
Format:	Thesis
Language:	English
Published:	2018
Subjects:	R Medicine RC109-216 Infectious and parasitic diseases
Online Access:	http://eprints.usm.my/56626/1/Dr.%20Wan%20Amani%20Wan%20Abdul%20Azim-24%20pages.pdf
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spelling	my-usm-ep.566262023-02-28T08:59:25Z A study on trichosporon isolation, its molecular identification, clinical manifestations and risk factors 2018 Azim, Wan Amani Wan Abdul R Medicine RC109-216 Infectious and parasitic diseases Trichosporon infection is a rare opportunistic, life threatening mycosis in patients with predisposing factors such as underlying immunodeficiency. Conventional methods to diagnose trichosporonosis are inadequate compared to molecular methods in terms of species identification. We reviewed 155 patients with Trichosporon isolations in hospital to study the proportion, clinical manifestations, outcome and risk factors for having Trichosporon infections. We found out that the majority species isolated were T. asahii (50.3%) and mostly were from urine samples (84.5%). Among risk factors for overall Trichosporon infections were ICU admission (95%CI 1.70, 10.37, p=0.002) and antifungal recipients (95%CI 0.06, 0.56, p=0.003). For Trichosporon fungemia, there were significant associations among those with hematological malignancy (p=0.001), antifungal recipients (p=0.002) and mortality (p=0.036). Mortality was observed more in fungemia compared to overall infection (55.6% vs. 22.5%). We also performed molecular identification on 39 available Trichosporon isolates (36 T. asahii, two T. mucoides and one T. inkin) by ITS1/4 sequencing that were previously identified by conventional method. We observed four discrepancies by conventional method where two T. asahii were identified as T. montevideense and two T. mucoides were T. debeurmannianum by ITS sequencing. In conclusion, Trichosporon infection in susceptible patients posed high mortality risk which required molecular identification for species identification to guide proper therapy. 2018 Thesis http://eprints.usm.my/56626/ http://eprints.usm.my/56626/1/Dr.%20Wan%20Amani%20Wan%20Abdul%20Azim-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine RC109-216 Infectious and parasitic diseases
spellingShingle	R Medicine RC109-216 Infectious and parasitic diseases Azim, Wan Amani Wan Abdul A study on trichosporon isolation, its molecular identification, clinical manifestations and risk factors
description	Trichosporon infection is a rare opportunistic, life threatening mycosis in patients with predisposing factors such as underlying immunodeficiency. Conventional methods to diagnose trichosporonosis are inadequate compared to molecular methods in terms of species identification. We reviewed 155 patients with Trichosporon isolations in hospital to study the proportion, clinical manifestations, outcome and risk factors for having Trichosporon infections. We found out that the majority species isolated were T. asahii (50.3%) and mostly were from urine samples (84.5%). Among risk factors for overall Trichosporon infections were ICU admission (95%CI 1.70, 10.37, p=0.002) and antifungal recipients (95%CI 0.06, 0.56, p=0.003). For Trichosporon fungemia, there were significant associations among those with hematological malignancy (p=0.001), antifungal recipients (p=0.002) and mortality (p=0.036). Mortality was observed more in fungemia compared to overall infection (55.6% vs. 22.5%). We also performed molecular identification on 39 available Trichosporon isolates (36 T. asahii, two T. mucoides and one T. inkin) by ITS1/4 sequencing that were previously identified by conventional method. We observed four discrepancies by conventional method where two T. asahii were identified as T. montevideense and two T. mucoides were T. debeurmannianum by ITS sequencing. In conclusion, Trichosporon infection in susceptible patients posed high mortality risk which required molecular identification for species identification to guide proper therapy.
format	Thesis
qualification_level	Master's degree
author	Azim, Wan Amani Wan Abdul
author_facet	Azim, Wan Amani Wan Abdul
author_sort	Azim, Wan Amani Wan Abdul
title	A study on trichosporon isolation, its molecular identification, clinical manifestations and risk factors
title_short	A study on trichosporon isolation, its molecular identification, clinical manifestations and risk factors
title_full	A study on trichosporon isolation, its molecular identification, clinical manifestations and risk factors
title_fullStr	A study on trichosporon isolation, its molecular identification, clinical manifestations and risk factors
title_full_unstemmed	A study on trichosporon isolation, its molecular identification, clinical manifestations and risk factors
title_sort	study on trichosporon isolation, its molecular identification, clinical manifestations and risk factors
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2018
url	http://eprints.usm.my/56626/1/Dr.%20Wan%20Amani%20Wan%20Abdul%20Azim-24%20pages.pdf
_version_	1776101167103737856

A study on trichosporon isolation, its molecular identification, clinical manifestations and risk factors

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