Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney

The present study investigated the effect of left ventricular hypertrophy (LVH) on the responsiveness of (II-adrenergic receptor subtypes to adrenergic stimuli in the rat. The role of hydrogen sulphide (H2S) and nitric oxide (NO) systems and their interaction in the progression of LVH was studied...

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Main Author:	Ahmad, Ashfaq
Format:	Thesis
Language:	English
Published:	2016
Subjects:	RS1-441 Pharmacy and materia medica
Online Access:	http://eprints.usm.my/57701/1/00001808094%20Ashfaq%20Ahmad24.pdf
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spelling	my-usm-ep.577012023-03-31T18:33:46Z Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney 2016-06 Ahmad, Ashfaq RS1-441 Pharmacy and materia medica The present study investigated the effect of left ventricular hypertrophy (LVH) on the responsiveness of (II-adrenergic receptor subtypes to adrenergic stimuli in the rat. The role of hydrogen sulphide (H2S) and nitric oxide (NO) systems and their interaction in the progression of LVH was studied by examining the effect of altered expression of cystathione y lyase (CSE mRNA) and endothelial nitric oxide synthase (eNOS mRN A) in the heart during LVH. Cardiovascular parameters such as cardiac geometry, oxidative stress, arterial stiffness and vascular responsiveness to vasoactive stimuli were studied. In addition, this study examined renal excretory functions, haemodynamics and histopathological changes after exogenous administration of NaHS, an H2S donor, L-arginine, an NO donor and a combination of NaHS and L-arginine. Wistar-Kyoto (WKY) rats were divided into two major groups of Control and LVH. These groups were then subdivided into 8 groups based on treatment. NaHS (56JlM/kg I.P. for 5 weeks) or L-arginine (1.25g1L for 5 weeks in drinking water) treated control or LVH groups. LVH was induced using isoprenaline (Smg/kg, S.c. every 72 hours for 2 weeks) and caffeine (62mglL in drinking water for 2 weeks). The uj-adrenergic receptors subtypes was studied by examining the responsiveness to noradrenaline (NA), phenylephrine (PE) and methoxamine (ME) in the presence of a background intrarenal infusion of selective uj-adrenergic receptors blockers (5-methylurapidil (5-MeU), chloroethy1clonidine (CEC) and BMY 7378). Real-time quantitative peR data (normalized to 2016-06 Thesis http://eprints.usm.my/57701/ http://eprints.usm.my/57701/1/00001808094%20Ashfaq%20Ahmad24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia. Pusat Pengajian Sains Farmasi
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RS1-441 Pharmacy and materia medica
spellingShingle	RS1-441 Pharmacy and materia medica Ahmad, Ashfaq Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney
description	The present study investigated the effect of left ventricular hypertrophy (LVH) on the responsiveness of (II-adrenergic receptor subtypes to adrenergic stimuli in the rat. The role of hydrogen sulphide (H2S) and nitric oxide (NO) systems and their interaction in the progression of LVH was studied by examining the effect of altered expression of cystathione y lyase (CSE mRNA) and endothelial nitric oxide synthase (eNOS mRN A) in the heart during LVH. Cardiovascular parameters such as cardiac geometry, oxidative stress, arterial stiffness and vascular responsiveness to vasoactive stimuli were studied. In addition, this study examined renal excretory functions, haemodynamics and histopathological changes after exogenous administration of NaHS, an H2S donor, L-arginine, an NO donor and a combination of NaHS and L-arginine. Wistar-Kyoto (WKY) rats were divided into two major groups of Control and LVH. These groups were then subdivided into 8 groups based on treatment. NaHS (56JlM/kg I.P. for 5 weeks) or L-arginine (1.25g1L for 5 weeks in drinking water) treated control or LVH groups. LVH was induced using isoprenaline (Smg/kg, S.c. every 72 hours for 2 weeks) and caffeine (62mglL in drinking water for 2 weeks). The uj-adrenergic receptors subtypes was studied by examining the responsiveness to noradrenaline (NA), phenylephrine (PE) and methoxamine (ME) in the presence of a background intrarenal infusion of selective uj-adrenergic receptors blockers (5-methylurapidil (5-MeU), chloroethy1clonidine (CEC) and BMY 7378). Real-time quantitative peR data (normalized to
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Ahmad, Ashfaq
author_facet	Ahmad, Ashfaq
author_sort	Ahmad, Ashfaq
title	Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney
title_short	Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney
title_full	Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney
title_fullStr	Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney
title_full_unstemmed	Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney
title_sort	interaction between hydrogen sulphide (h2s) and nitric oxide (no) in left ventricular hypertrophy and their effect on responsiveness of alpha i-adrenergic receptors subtypes in the rat kidney
granting_institution	Universiti Sains Malaysia.
granting_department	Pusat Pengajian Sains Farmasi
publishDate	2016
url	http://eprints.usm.my/57701/1/00001808094%20Ashfaq%20Ahmad24.pdf
_version_	1776101207196041216

Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney

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