Intra-subject investigation into the role of dental plaque induced IL-8, IL-6 and IL-1α expression and its association with the subgingival plaque microbiome

Aims: The study aims to evaluate the subgingival plaque microbiome composition and measure the GCF level of the IL-8, IL-6, and IL-1α of healthy and periodontitis sites in the same periodontitis subject. The study also explored the association between subgingival plaque composition and GCF levels...

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Main Author:	Tariq, Maheen
Format:	Thesis
Language:	English
Published:	2022
Subjects:	RK1-715 Dentistry
Online Access:	http://eprints.usm.my/57755/1/MAHEEN%20TARIQ-FINAL%20THESIS%20P-SGM001218%28R%29%20-24%20pages.pdf
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spelling	my-usm-ep.577552023-04-11T01:10:56Z Intra-subject investigation into the role of dental plaque induced IL-8, IL-6 and IL-1α expression and its association with the subgingival plaque microbiome 2022-01 Tariq, Maheen RK1-715 Dentistry Aims: The study aims to evaluate the subgingival plaque microbiome composition and measure the GCF level of the IL-8, IL-6, and IL-1α of healthy and periodontitis sites in the same periodontitis subject. The study also explored the association between subgingival plaque composition and GCF levels of IL-8, IL-6, and IL-1α at periodontitis and healthy sites. Materials and methods: This study included 34 periodontitis subjects to collect the subgingival plaque and GCF samples from the periodontitis and healthy sites. The extracted DNA from dental plaque was sent to Illumina lab for 16s rRNA metagenomics. The V₃ and V₄ region of 16s RNA were amplified. The library was sequenced with MiSeq System, and data was analysed using MiSeq Reporter software (Illumina, San Diego, CA). After, quality filtering 313480 sequences were obtained and arranged in OTU based on 97% threshold. The GCF samples were analysed using ELISA to measure the level of IL-8, IL-6 and IL- 1α. The optical density was determined, and standard curve was generated using a four-parameter logistic curve fit. Results: The analysis of 16s rRNA revealed that periodontitis sites showed a higher species diversity than healthy sites. Moreover, there was no apparent clustering of healthy and periodontitis samples. Total 19 phylum, 142 genera, and 56 species were detected. The ELISA results show a trend of higher IL-8 and IL-6 concentrations in periodontitis sites and higher IL-1 concentrations in healthy sites. However, the differences between healthy and periodontitis sites were not statistically significant. Moreover, there was also a trend of positive and a negative correlation between periodontal parameters (PPD, CAL, and BOP) with IL-8, IL-6, and IL-1α. However, these findings were not statistically significant. The correlation between IL-8, IL-6, and IL-1α and bacterial species was also investigated. Our results show a statistically significant correlation (a mix of negative and positive correlation) between some bacteria species and cytokines (IL-8, IL-6 and IL-1α). For example, at the periodontitis site, IL-8 showed a statistically significant strong positive correlation with Bacillus thermoamylovorans. Conclusion: These findings suggests that certain microbiome may possibly cause the host immune system to secrete pro-inflammatory cytokines such as IL-8, IL-6, and IL-1α. Moreover, disease-related bacteria such as Phlyum (Spirochaetes, TM7 and Synergestetes), genus (Porphyromonas, Filifactor, and Peptococcus) and species (Prevotella intermedia, Prevotella copri, Prevotella nanceiensis, and Treponema socranskii) may be found abundantly in healthy sites and may help in the shift from healthy to periodontal disease. 2022-01 Thesis http://eprints.usm.my/57755/ http://eprints.usm.my/57755/1/MAHEEN%20TARIQ-FINAL%20THESIS%20P-SGM001218%28R%29%20-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Pergigian
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RK1-715 Dentistry
spellingShingle	RK1-715 Dentistry Tariq, Maheen Intra-subject investigation into the role of dental plaque induced IL-8, IL-6 and IL-1α expression and its association with the subgingival plaque microbiome
description	Aims: The study aims to evaluate the subgingival plaque microbiome composition and measure the GCF level of the IL-8, IL-6, and IL-1α of healthy and periodontitis sites in the same periodontitis subject. The study also explored the association between subgingival plaque composition and GCF levels of IL-8, IL-6, and IL-1α at periodontitis and healthy sites. Materials and methods: This study included 34 periodontitis subjects to collect the subgingival plaque and GCF samples from the periodontitis and healthy sites. The extracted DNA from dental plaque was sent to Illumina lab for 16s rRNA metagenomics. The V₃ and V₄ region of 16s RNA were amplified. The library was sequenced with MiSeq System, and data was analysed using MiSeq Reporter software (Illumina, San Diego, CA). After, quality filtering 313480 sequences were obtained and arranged in OTU based on 97% threshold. The GCF samples were analysed using ELISA to measure the level of IL-8, IL-6 and IL- 1α. The optical density was determined, and standard curve was generated using a four-parameter logistic curve fit. Results: The analysis of 16s rRNA revealed that periodontitis sites showed a higher species diversity than healthy sites. Moreover, there was no apparent clustering of healthy and periodontitis samples. Total 19 phylum, 142 genera, and 56 species were detected. The ELISA results show a trend of higher IL-8 and IL-6 concentrations in periodontitis sites and higher IL-1 concentrations in healthy sites. However, the differences between healthy and periodontitis sites were not statistically significant. Moreover, there was also a trend of positive and a negative correlation between periodontal parameters (PPD, CAL, and BOP) with IL-8, IL-6, and IL-1α. However, these findings were not statistically significant. The correlation between IL-8, IL-6, and IL-1α and bacterial species was also investigated. Our results show a statistically significant correlation (a mix of negative and positive correlation) between some bacteria species and cytokines (IL-8, IL-6 and IL-1α). For example, at the periodontitis site, IL-8 showed a statistically significant strong positive correlation with Bacillus thermoamylovorans. Conclusion: These findings suggests that certain microbiome may possibly cause the host immune system to secrete pro-inflammatory cytokines such as IL-8, IL-6, and IL-1α. Moreover, disease-related bacteria such as Phlyum (Spirochaetes, TM7 and Synergestetes), genus (Porphyromonas, Filifactor, and Peptococcus) and species (Prevotella intermedia, Prevotella copri, Prevotella nanceiensis, and Treponema socranskii) may be found abundantly in healthy sites and may help in the shift from healthy to periodontal disease.
format	Thesis
qualification_level	Master's degree
author	Tariq, Maheen
author_facet	Tariq, Maheen
author_sort	Tariq, Maheen
title	Intra-subject investigation into the role of dental plaque induced IL-8, IL-6 and IL-1α expression and its association with the subgingival plaque microbiome
title_short	Intra-subject investigation into the role of dental plaque induced IL-8, IL-6 and IL-1α expression and its association with the subgingival plaque microbiome
title_full	Intra-subject investigation into the role of dental plaque induced IL-8, IL-6 and IL-1α expression and its association with the subgingival plaque microbiome
title_fullStr	Intra-subject investigation into the role of dental plaque induced IL-8, IL-6 and IL-1α expression and its association with the subgingival plaque microbiome
title_full_unstemmed	Intra-subject investigation into the role of dental plaque induced IL-8, IL-6 and IL-1α expression and its association with the subgingival plaque microbiome
title_sort	intra-subject investigation into the role of dental plaque induced il-8, il-6 and il-1α expression and its association with the subgingival plaque microbiome
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Pergigian
publishDate	2022
url	http://eprints.usm.my/57755/1/MAHEEN%20TARIQ-FINAL%20THESIS%20P-SGM001218%28R%29%20-24%20pages.pdf
_version_	1776101208882151424

Intra-subject investigation into the role of dental plaque induced IL-8, IL-6 and IL-1α expression and its association with the subgingival plaque microbiome

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