Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation
Glucoamylase enzyme is widely utilized for conversion of starch into glucose during saccharification process. Aspergillus niger van Tieghem is commonly used for glucoamylase production in submerged cultivation system (SmF) and solid state fermentation (SSF). This research was carried out to enhan...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | http://eprints.usm.my/59458/1/24%20Pages%20from%2000001760344.pdf |
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| Summary: | Glucoamylase enzyme is widely utilized for conversion of starch into glucose during
saccharification process. Aspergillus niger van Tieghem is commonly used for
glucoamylase production in submerged cultivation system (SmF) and solid state
fermentation (SSF). This research was carried out to enhance glucoamylase
production by mutating A. niger van Tieghem using chemical mutagen, ethyl
methane sulphonate (EMS) via random mutation. Mutant ANC9 was identified as a
potential mutant which produced, 1.9 and 1.6 fold higher glucoamylase activity
towards cooked starch and raw starch respectively in SmF using cooked starch as
substrate. Glucoamylase activity towards raw starch and cooked starch in SSF by
mutant ANC9 were 44.81± 1.2 Ulg and 101.50 ±2.2 Ulg respectively compared to
wild strain which was only able to produce 34.4±O.5 Ulg towards cooked starch and
79.1±2.2 Ulg towards raw starch. |
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