Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing

Tapinoma indicum Forel (Hymenoptera: Formicidae) is a household pest that is widely distributed in Asian countries. It is known as a nuisance pest that causes annoyance and disturbance by constructing nests and foraging in building for food and water. However, studies on T. indicum are limited, e...

Full description

Saved in:

Bibliographic Details
Main Author:	Lim, Li Yang
Format:	Thesis
Language:	English
Published:	2022
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/59706/1/24%20Pages%20from%20LIM%20LI%20YANG%20-%20TESIS.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.59706
record_format	uketd_dc
spelling	my-usm-ep.597062023-12-19T04:16:29Z Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing 2022-09 Lim, Li Yang QH1-278.5 Natural history (General) Tapinoma indicum Forel (Hymenoptera: Formicidae) is a household pest that is widely distributed in Asian countries. It is known as a nuisance pest that causes annoyance and disturbance by constructing nests and foraging in building for food and water. However, studies on T. indicum are limited, especially in the field of molecular biology. Hence, this research aims to study the T. indicum population genetic structure in Penang Island, Malaysia by using the novel microsatellite markers developed from whole genome of T. indium. Objectives of this research include: to determine the T. indicum population distribution in Penang island, Malaysia; to identify the phylogenetic relationship of T. indicum based on the mitochondrial gene of cytochrome c oxidase subunit Ⅰ (CO1) and 16S ribosomal DNA (16S rDNA); to design novel microsatellite marker by screening microsatellite loci of T. indicum genome sequenced from next-generation sequencing; to validate polymorphism of designed microsatellite marker; to analyse genetic diversity and population genetic structure of T. indicum using designed microsatellite markers. There was a total of 30 populations collected from Balik Pulau, Gelugor and George Town. The CO1 sequences and 16S rDNA sequences of T. indicum from each population were deposited and accessible via Genbank (NCBI) database. 2022-09 Thesis http://eprints.usm.my/59706/ http://eprints.usm.my/59706/1/24%20Pages%20from%20LIM%20LI%20YANG%20-%20TESIS.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Lim, Li Yang Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing
description	Tapinoma indicum Forel (Hymenoptera: Formicidae) is a household pest that is widely distributed in Asian countries. It is known as a nuisance pest that causes annoyance and disturbance by constructing nests and foraging in building for food and water. However, studies on T. indicum are limited, especially in the field of molecular biology. Hence, this research aims to study the T. indicum population genetic structure in Penang Island, Malaysia by using the novel microsatellite markers developed from whole genome of T. indium. Objectives of this research include: to determine the T. indicum population distribution in Penang island, Malaysia; to identify the phylogenetic relationship of T. indicum based on the mitochondrial gene of cytochrome c oxidase subunit Ⅰ (CO1) and 16S ribosomal DNA (16S rDNA); to design novel microsatellite marker by screening microsatellite loci of T. indicum genome sequenced from next-generation sequencing; to validate polymorphism of designed microsatellite marker; to analyse genetic diversity and population genetic structure of T. indicum using designed microsatellite markers. There was a total of 30 populations collected from Balik Pulau, Gelugor and George Town. The CO1 sequences and 16S rDNA sequences of T. indicum from each population were deposited and accessible via Genbank (NCBI) database.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Lim, Li Yang
author_facet	Lim, Li Yang
author_sort	Lim, Li Yang
title	Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing
title_short	Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing
title_full	Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing
title_fullStr	Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing
title_full_unstemmed	Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing
title_sort	population genetic study of tapinoma indicum (forel) in penang island, malaysia using microsatellite marker developed by nextgeneration sequencing
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2022
url	http://eprints.usm.my/59706/1/24%20Pages%20from%20LIM%20LI%20YANG%20-%20TESIS.pdf
_version_	1794024039914668032

Population Genetic Study Of Tapinoma Indicum (Forel) In Penang Island, Malaysia Using Microsatellite Marker Developed By Nextgeneration Sequencing

Similar Items