Prevalence of cytomegalovirus (CMV) infection among infants and correlation between cmv pcr with clinical outcomes in Hospital Universiti Sains Malaysia (HUSM)

Introduction: Congenital Cytomegalovirus (CMV) infection is the leading infectious cause of congenital hearing loss and neurodevelopmental disability. The aims of this study were to describe the prevalence and clinical manifestations of infants with CMV infection in Hospital Universiti Sains Mala...

Full description

Saved in:

Bibliographic Details
Main Author:	Norjihan, Abdul Hamid
Format:	Thesis
Language:	English
Published:	2020
Subjects:	R Medicine RC109-216 Infectious and parasitic diseases
Online Access:	http://eprints.usm.my/60608/1/NORJIHAN%20BINTI%20AB%20HAMID-E.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.60608
record_format	uketd_dc
spelling	my-usm-ep.606082024-05-19T07:20:23Z Prevalence of cytomegalovirus (CMV) infection among infants and correlation between cmv pcr with clinical outcomes in Hospital Universiti Sains Malaysia (HUSM) 2020 Norjihan, Abdul Hamid R Medicine R735-854 Medical education. Medical schools. Research RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RC109-216 Infectious and parasitic diseases Introduction: Congenital Cytomegalovirus (CMV) infection is the leading infectious cause of congenital hearing loss and neurodevelopmental disability. The aims of this study were to describe the prevalence and clinical manifestations of infants with CMV infection in Hospital Universiti Sains Malaysia (HUSM) and to determine its correlation between CMV PCR with clinical outcomes. Materials and Methods: A total of 648 blood samples of infants requested for TORCHES screening from January 2018 to December 2018 were sent to Microbiology laboratory in Hospital Universiti Sains Malaysia (HUSM). The samples were tested for anti-CMV IgM and IgG by electrochemiluminiscence immunoassay (ECLIA) method. Infants with first serum sample of IgM and IgG antibody titre suggestive of Cytomegalovirus infection were requested for second serum sample at two to four weeks interval together with plasma samples for Cytomegalovirus DNA viral load and maternal sample for serological analysis. The plasma CMV DNA quantification was performed using real-time PCR assays. Correlation of CMV viral load with clinical outcomes were analysed using the point- biserial correlation Results: The prevalence of symptomatic Cytomegalovirus infection among infant in HUSM was 6.48 % (42/648). Out of 648 cases, 196 cases had paired sera samples and 97 cases were tested for CMV PCR. Only 39 cases were positive for CMV PCR. Based on serological and PCR results, the cases were categorised into three groups: acute CMV infection (n = 42), passive immunity (n = 113) and inconclusive (n =41). Clinical presentations for acute Cytomegalovirus infection were small for gestational age (64%), jaundice (52.3%), presumed sepsis (21.4%), abnormal hearing assessment (9.5%), hepatitis (9.5%), ocular problem (4.7%), prematurity (4.7%), rash (2%), microcephaly (2.3%), cerebral calcification (2.3%) and thrombocytopenia (2.3%). There was positive moderate significant correlation between CMV viral load and abnormal hearing assessment and weak positive significant correlation between CMV viral load and abnormal ocular finding. Conclusion: The prevalence of CMV infection among infants in our center was comparable with previous studies. However, the prevalence of cCMV among infants was lower that might be due underdiagnosed. Abnormal hearing assessment, presumed sepsis, CMV hepatitis and rash were significantly associated with acute symptomatic CMV infection among infants. There were significant correlation between CMV viral load with abnormal hearing assessment and abnormal ocular findings. 2020 Thesis http://eprints.usm.my/60608/ http://eprints.usm.my/60608/1/NORJIHAN%20BINTI%20AB%20HAMID-E.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine R Medicine R Medicine RC109-216 Infectious and parasitic diseases
spellingShingle	R Medicine R Medicine R Medicine RC109-216 Infectious and parasitic diseases Norjihan, Abdul Hamid Prevalence of cytomegalovirus (CMV) infection among infants and correlation between cmv pcr with clinical outcomes in Hospital Universiti Sains Malaysia (HUSM)
description	Introduction: Congenital Cytomegalovirus (CMV) infection is the leading infectious cause of congenital hearing loss and neurodevelopmental disability. The aims of this study were to describe the prevalence and clinical manifestations of infants with CMV infection in Hospital Universiti Sains Malaysia (HUSM) and to determine its correlation between CMV PCR with clinical outcomes. Materials and Methods: A total of 648 blood samples of infants requested for TORCHES screening from January 2018 to December 2018 were sent to Microbiology laboratory in Hospital Universiti Sains Malaysia (HUSM). The samples were tested for anti-CMV IgM and IgG by electrochemiluminiscence immunoassay (ECLIA) method. Infants with first serum sample of IgM and IgG antibody titre suggestive of Cytomegalovirus infection were requested for second serum sample at two to four weeks interval together with plasma samples for Cytomegalovirus DNA viral load and maternal sample for serological analysis. The plasma CMV DNA quantification was performed using real-time PCR assays. Correlation of CMV viral load with clinical outcomes were analysed using the point- biserial correlation Results: The prevalence of symptomatic Cytomegalovirus infection among infant in HUSM was 6.48 % (42/648). Out of 648 cases, 196 cases had paired sera samples and 97 cases were tested for CMV PCR. Only 39 cases were positive for CMV PCR. Based on serological and PCR results, the cases were categorised into three groups: acute CMV infection (n = 42), passive immunity (n = 113) and inconclusive (n =41). Clinical presentations for acute Cytomegalovirus infection were small for gestational age (64%), jaundice (52.3%), presumed sepsis (21.4%), abnormal hearing assessment (9.5%), hepatitis (9.5%), ocular problem (4.7%), prematurity (4.7%), rash (2%), microcephaly (2.3%), cerebral calcification (2.3%) and thrombocytopenia (2.3%). There was positive moderate significant correlation between CMV viral load and abnormal hearing assessment and weak positive significant correlation between CMV viral load and abnormal ocular finding. Conclusion: The prevalence of CMV infection among infants in our center was comparable with previous studies. However, the prevalence of cCMV among infants was lower that might be due underdiagnosed. Abnormal hearing assessment, presumed sepsis, CMV hepatitis and rash were significantly associated with acute symptomatic CMV infection among infants. There were significant correlation between CMV viral load with abnormal hearing assessment and abnormal ocular findings.
format	Thesis
qualification_level	Master's degree
author	Norjihan, Abdul Hamid
author_facet	Norjihan, Abdul Hamid
author_sort	Norjihan, Abdul Hamid
title	Prevalence of cytomegalovirus (CMV) infection among infants and correlation between cmv pcr with clinical outcomes in Hospital Universiti Sains Malaysia (HUSM)
title_short	Prevalence of cytomegalovirus (CMV) infection among infants and correlation between cmv pcr with clinical outcomes in Hospital Universiti Sains Malaysia (HUSM)
title_full	Prevalence of cytomegalovirus (CMV) infection among infants and correlation between cmv pcr with clinical outcomes in Hospital Universiti Sains Malaysia (HUSM)
title_fullStr	Prevalence of cytomegalovirus (CMV) infection among infants and correlation between cmv pcr with clinical outcomes in Hospital Universiti Sains Malaysia (HUSM)
title_full_unstemmed	Prevalence of cytomegalovirus (CMV) infection among infants and correlation between cmv pcr with clinical outcomes in Hospital Universiti Sains Malaysia (HUSM)
title_sort	prevalence of cytomegalovirus (cmv) infection among infants and correlation between cmv pcr with clinical outcomes in hospital universiti sains malaysia (husm)
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2020
url	http://eprints.usm.my/60608/1/NORJIHAN%20BINTI%20AB%20HAMID-E.pdf
_version_	1804888973393264640

Prevalence of cytomegalovirus (CMV) infection among infants and correlation between cmv pcr with clinical outcomes in Hospital Universiti Sains Malaysia (HUSM)

Similar Items