Evaluation of prime boosting vaccination strategy using newly constructed tuberculosis vaccine candidates in mice
Tuberculosis (TB) an infectious disease caused by Mycobacterium tuberculosis complex continues to be major health problem, worldwide. Current the only available preventive TB vaccine used is Bacillus Calmette Guerin (BCG) but unfortunately, the efficacy of BCG nowdays is controversial. Different v...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://eprints.usm.my/60877/1/NURAZWANA%20BINTI%20ZAKARIA%20-%20e.pdf |
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| Summary: | Tuberculosis (TB) an infectious disease caused by Mycobacterium tuberculosis complex continues to be major health problem, worldwide. Current the
only available preventive TB vaccine used is Bacillus Calmette Guerin (BCG) but
unfortunately, the efficacy of BCG nowdays is controversial. Different vaccine delivery approaches have been developed based on the available technologies. In
this study, we are using two previously constructed vaccine candidates namely
VacIV DNA vaccine and StVacIII surface display vaccine, together with standard BCG vaccine employing prime boosting vaccination strategy. VacIV DNA vaccine
was given intramuscularly to mice while StVacIII surface display vaccine and BCG
was given orally. Mice whole blood and splenocytes from the vaccinated mice were
tested for various immunological tests. The results showed that mice whole blood
(peripheral blood) and splenocytes from the immunized mice were found to increase
the production of IL-2 and IFN-y when stimulated with the antigen (Mtb 8.4) which
is one of the epitopes in both VacIV and VacIII DNA vaccine. Flow cytometric
intracellular cytokine analysis of splenocytes from vaccinated mice showed that both
CD4+ and CD8+ T cells produce IL-2 and IFN-y upon stimulation with the antigens.
The same responses also were seen in peripheral blood. In the prime-boost approach,
the study showed that mice primed using StVacIII surface display vaccine and
boosted with VacIV DNA vaccine is a better strategy in increasing the immune
response in mice. In conclusion, the data obtained from this study suggested that surface display vaccine in combination with DNA vaccine using prime-boost
vaccination strategy gives new ideas in vaccine development against tuberculosis.
Further study is required to confirm the efficacy of the prime-boosting vaccination
strategy in term of protection in animal model. |
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