Evaluation of prime boosting vaccination strategy using newly constructed tuberculosis vaccine candidates in mice

Tuberculosis (TB) an infectious disease caused by Mycobacterium tuberculosis complex continues to be major health problem, worldwide. Current the only available preventive TB vaccine used is Bacillus Calmette Guerin (BCG) but unfortunately, the efficacy of BCG nowdays is controversial. Different v...

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Main Author:	Zakaria, Nurazwana
Format:	Thesis
Language:	English
Published:	2013
Subjects:	R Medicine RC306-320.5 Tuberculosis
Online Access:	http://eprints.usm.my/60877/1/NURAZWANA%20BINTI%20ZAKARIA%20-%20e.pdf
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id	my-usm-ep.60877
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spelling	my-usm-ep.608772024-11-14T03:43:27Z Evaluation of prime boosting vaccination strategy using newly constructed tuberculosis vaccine candidates in mice 2013 Zakaria, Nurazwana R Medicine RC306-320.5 Tuberculosis Tuberculosis (TB) an infectious disease caused by Mycobacterium tuberculosis complex continues to be major health problem, worldwide. Current the only available preventive TB vaccine used is Bacillus Calmette Guerin (BCG) but unfortunately, the efficacy of BCG nowdays is controversial. Different vaccine delivery approaches have been developed based on the available technologies. In this study, we are using two previously constructed vaccine candidates namely VacIV DNA vaccine and StVacIII surface display vaccine, together with standard BCG vaccine employing prime boosting vaccination strategy. VacIV DNA vaccine was given intramuscularly to mice while StVacIII surface display vaccine and BCG was given orally. Mice whole blood and splenocytes from the vaccinated mice were tested for various immunological tests. The results showed that mice whole blood (peripheral blood) and splenocytes from the immunized mice were found to increase the production of IL-2 and IFN-y when stimulated with the antigen (Mtb 8.4) which is one of the epitopes in both VacIV and VacIII DNA vaccine. Flow cytometric intracellular cytokine analysis of splenocytes from vaccinated mice showed that both CD4+ and CD8+ T cells produce IL-2 and IFN-y upon stimulation with the antigens. The same responses also were seen in peripheral blood. In the prime-boost approach, the study showed that mice primed using StVacIII surface display vaccine and boosted with VacIV DNA vaccine is a better strategy in increasing the immune response in mice. In conclusion, the data obtained from this study suggested that surface display vaccine in combination with DNA vaccine using prime-boost vaccination strategy gives new ideas in vaccine development against tuberculosis. Further study is required to confirm the efficacy of the prime-boosting vaccination strategy in term of protection in animal model. 2013 Thesis http://eprints.usm.my/60877/ http://eprints.usm.my/60877/1/NURAZWANA%20BINTI%20ZAKARIA%20-%20e.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine RC306-320.5 Tuberculosis
spellingShingle	R Medicine RC306-320.5 Tuberculosis Zakaria, Nurazwana Evaluation of prime boosting vaccination strategy using newly constructed tuberculosis vaccine candidates in mice
description	Tuberculosis (TB) an infectious disease caused by Mycobacterium tuberculosis complex continues to be major health problem, worldwide. Current the only available preventive TB vaccine used is Bacillus Calmette Guerin (BCG) but unfortunately, the efficacy of BCG nowdays is controversial. Different vaccine delivery approaches have been developed based on the available technologies. In this study, we are using two previously constructed vaccine candidates namely VacIV DNA vaccine and StVacIII surface display vaccine, together with standard BCG vaccine employing prime boosting vaccination strategy. VacIV DNA vaccine was given intramuscularly to mice while StVacIII surface display vaccine and BCG was given orally. Mice whole blood and splenocytes from the vaccinated mice were tested for various immunological tests. The results showed that mice whole blood (peripheral blood) and splenocytes from the immunized mice were found to increase the production of IL-2 and IFN-y when stimulated with the antigen (Mtb 8.4) which is one of the epitopes in both VacIV and VacIII DNA vaccine. Flow cytometric intracellular cytokine analysis of splenocytes from vaccinated mice showed that both CD4+ and CD8+ T cells produce IL-2 and IFN-y upon stimulation with the antigens. The same responses also were seen in peripheral blood. In the prime-boost approach, the study showed that mice primed using StVacIII surface display vaccine and boosted with VacIV DNA vaccine is a better strategy in increasing the immune response in mice. In conclusion, the data obtained from this study suggested that surface display vaccine in combination with DNA vaccine using prime-boost vaccination strategy gives new ideas in vaccine development against tuberculosis. Further study is required to confirm the efficacy of the prime-boosting vaccination strategy in term of protection in animal model.
format	Thesis
qualification_level	Master's degree
author	Zakaria, Nurazwana
author_facet	Zakaria, Nurazwana
author_sort	Zakaria, Nurazwana
title	Evaluation of prime boosting vaccination strategy using newly constructed tuberculosis vaccine candidates in mice
title_short	Evaluation of prime boosting vaccination strategy using newly constructed tuberculosis vaccine candidates in mice
title_full	Evaluation of prime boosting vaccination strategy using newly constructed tuberculosis vaccine candidates in mice
title_fullStr	Evaluation of prime boosting vaccination strategy using newly constructed tuberculosis vaccine candidates in mice
title_full_unstemmed	Evaluation of prime boosting vaccination strategy using newly constructed tuberculosis vaccine candidates in mice
title_sort	evaluation of prime boosting vaccination strategy using newly constructed tuberculosis vaccine candidates in mice
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kesihatan
publishDate	2013
url	http://eprints.usm.my/60877/1/NURAZWANA%20BINTI%20ZAKARIA%20-%20e.pdf
_version_	1818647357908058112

Evaluation of prime boosting vaccination strategy using newly constructed tuberculosis vaccine candidates in mice

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